Dynamic interplay between target search and recognition for a Type I CRISPR-Cas system

Autor: Pierre Aldag, Marius Rutkauskas, Julene Madariaga-Marcos, Inga Songailiene, Tomas Sinkunas, Felix Kemmerich, Dominik Kauert, Virginijus Siksnys, Ralf Seidel

Publikováno v: Nature Communications, Vol 14, Iss 1, Pp 1-14 (2023)

Abstract CRISPR-Cas effector complexes enable the defense against foreign nucleic acids and have recently been exploited as molecular tools for precise genome editing at a target locus. To bind and cleave their target, the CRISPR-Cas effectors have t

Externí odkaz: https://doaj.org/article/e871251abb314dc899785b43baed2dc2

Disarming of type I-F CRISPR-Cas surveillance complex by anti-CRISPR proteins AcrIF6 and AcrIF9

Autor: Egle Kupcinskaite, Marijonas Tutkus, Aurimas Kopūstas, Simonas Ašmontas, Marija Jankunec, Mindaugas Zaremba, Giedre Tamulaitiene, Tomas Sinkunas

Publikováno v: Scientific Reports, Vol 12, Iss 1, Pp 1-14 (2022)

Abstract CRISPR-Cas systems are prokaryotic adaptive immune systems that protect against phages and other invading nucleic acids. The evolutionary arms race between prokaryotes and phages gave rise to phage anti-CRISPR (Acr) proteins that act as a co

Externí odkaz: https://doaj.org/article/ae7f2c66b11e44f39960982c86ff9f9e

Decision-Making in Cascade Complexes Harboring crRNAs of Altered Length

Autor: Inga Songailiene, Marius Rutkauskas, Tomas Sinkunas, Elena Manakova, Sabine Wittig, Carla Schmidt, Virginijus Siksnys, Ralf Seidel

Publikováno v: Cell Reports, Vol 28, Iss 12, Pp 3157-3166.e4 (2019)

Summary: The multi-subunit type I CRISPR-Cas surveillance complex Cascade uses its crRNA to recognize dsDNA targets. Recognition involves DNA unwinding and base-pairing between the crRNA spacer region and a complementary DNA strand, resulting in form

Externí odkaz: https://doaj.org/article/a545ce10365146db92a8077000d6b635

The energy landscape for R-loop formation by the CRISPR-Cas Cascade complex

Autor: Dominik J. Kauert, Julene Madariaga-Marcos, Marius Rutkauskas, Alexander Wulfken, Inga Songailiene, Tomas Sinkunas, Virginijus Siksnys, Ralf Seidel

The discovery1,2and the pioneering applications3of CRISPR-Cas effector complexes have provided powerful gene-editing tools. The effector complexes are guided to the targeted genomic locus by the complementarity of their CRISPR RNA (crRNA)4,5. Recogni

Externí odkaz: https://explore.openaire.eu/search/publication?articleId=doi_________::4268094fd7bb461ed9a926cdc5c570ba
https://doi.org/10.1101/2023.03.17.533087

DNA interference is controlled by R-loop length in a type I-F1 CRISPR-Cas system

Autor: Marija Fiodorovaite, Donata Tuminauskaite, Danguole Norkunaite, Sarunas Tumas, Tomas Sinkunas, Giedre Tamulaitiene, Inga Songailiene

Publikováno v: BMC Biology
BMC Biology, Vol 18, Iss 1, Pp 1-16 (2020)
BMC Biology, London : BMC, 2020, vol.18, art. no. 65, p. [1-16]
BMC Biology, London : BMC, 2020, vol.18, art. no65, p. [1-16]

BackgroundCRISPR-Cas systems, which provide adaptive immunity against foreign nucleic acids in prokaryotes, can serve as useful molecular tools for multiple applications in genome engineering. Diverse CRISPR-Cas systems originating from distinct prok

Externí odkaz: https://explore.openaire.eu/search/publication?articleId=doi_dedup___::4222154f894c4006bb5b6509d1d433a5
http://europepmc.org/articles/PMC7296934