Zobrazeno 1 - 10 of 20 pro vyhledávání: '"Tomas Kempe"'
1
Cleavage of oligodeoxyribonucleotides from controlled-pore glass supports and their rapid deprotection by gaseous amines
Autor: Andrzej Wilk, Nagaradona Harindranath, Serge L. Beaucage, Tomas Kempe, Jila H. Boal, Edward E. Max
Publikováno v: Nucleic Acids Research. 24:3115-3117
A novel method for the deprotection of oligodeoxyribonucleotides has been developed. Gaseous amines such as ammonia or methylamine were employed under pressure to achieve mild and rapid deprotection conditions. For example, oligodeoxyribonucleotides
Externí odkaz: https://explore.openaire.eu/search/publication?articleId=doi_dedup___::c945de9eb6ecbbeffd685361a537c201
https://doi.org/10.1093/nar/24.15.3115
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2
Selective 2′-benzoylation at the 2′, 3′-diols of protected ribonucleosides. New solid phase synthesis of RNA and DNA-RNA mixtures
Autor: Susan M. Peterson, Thomas J. Nardi, Tomas Kempe, Brad Paulson, Wesley I. Sundquist, Flora Chow
Publikováno v: Nucleic Acids Research. 10:6695-6714
5'-0-(Dimethoxytrityl)-2'-0-(benzoyl or 3,4,5-trimethoxybenzoyl)-base protected ribonucleosides have been prepared by selective benzoylation of the 2'-hydroxyl group. The isomerization of the 2'-benzoates to the 3'-benzoates was studied. The protecte
Externí odkaz: https://explore.openaire.eu/search/publication?articleId=doi_dedup___::d3776d42fdee474cbe511ec897a29e27
https://doi.org/10.1093/nar/10.21.6695
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3
Chemical and enzymatic biotin-labeling of oligodeoxyribonucleotides
Autor: Tomas Kempe, Wesley I. Sundquist, Flora Chow, Shiu-Lok Hu
Publikováno v: Nucleic Acids Research. 13:45-57
Biotin has been converted to 2-(biotinylamido)ethanol and condensed to phosphorylated oligonucleotides in a solid phase synthesis. The 5'-biotinylated oligonucleotides were enzymatically coupled to other DNA fragments by T4 DNA ligase or T4 RNA ligas
Externí odkaz: https://explore.openaire.eu/search/publication?articleId=doi_dedup___::185ed868b668ddc8aa1173cc694c7ea5
https://doi.org/10.1093/nar/13.1.45
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4
Production and Characterization of Growth Hormone Releasing Factor Analogs Through Recombinant DNA and Chemical Techniques
Autor: Flora Chow, Gary Opperman, Pamela Baker, Gary Long, James J. L'Italien, Susan M. Peterson, William Hays, Tomas Kempe, Brad Paulson
Publikováno v: Nature Biotechnology. 4:565-568
A growth hormone releasing factor analog [Leu27,Hse44]GRF(1–44)lactone has been made by recombinant DNA techniques. The peptide was expressed from a multiple–copied GRF gene fused to lacZ. The monomeric GRF peptide analog with a C–terminal homo
Externí odkaz: https://explore.openaire.eu/search/publication?articleId=doi_________::4a697a8374664b72bdbb47d11e451e65
https://doi.org/10.1038/nbt0686-565
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6
Silver(I) ion catalyzed rearrangements of strained .sigma. bonds. 33. Benzoannulation as a probe of structural dimension and .pi.-electron distribution in elassovalenes. A mathematical model for the assessment of homoaromaticity
Autor: Thomas G. Wallis, Tomas Kempe, Gary G. Christoph, James P. Springer, Jon Clardy, Leo A. Paquette
Publikováno v: Journal of the American Chemical Society. 99:6946-6954
6,7-Benzoelassovalene has been synthesized from benzocyclobutene and 1,4-cyclohexadiene-1,2-dicarboxylic anhydride. This crystalline white solid undergoes rapid decomposition when exposed to air, but can be handled under an argon atmosphere. An x-ray
Externí odkaz: https://explore.openaire.eu/search/publication?articleId=doi_________::9e5f88cb973bc38715ee261bb31b7458
https://doi.org/10.1021/ja00463a029
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9
[Homoserine31]-Salmon Calcitonin I: Fully Active Analogue of Calcitonin Synthesized by Recombinant DNA Techniques
Autor: Hunter Heath, David N Fass, Flora Chow, Tomas Kempe
Publikováno v: Nature Biotechnology. 6:190-192
We have made an active analogue of salmon calcitonin I, [homoserine 31]–salmon calcitonin I, by recombinant DNA and chemical techniques. First a calcitonin precursor, the [homoserine31]–salmon calcitonin I–(1–31) lactone, was obtained from a
Externí odkaz: https://explore.openaire.eu/search/publication?articleId=doi_________::85ea7d65350d2cd1fd69fd3eb2df7c68
https://doi.org/10.1038/nbt0288-190
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