Dual client binding sites in the ATP-independent chaperone SurA

Autor: Bob Schiffrin, Joel A. Crossley, Martin Walko, Jonathan M. Machin, G. Nasir Khan, Iain W. Manfield, Andrew J. Wilson, David J. Brockwell, Tomas Fessl, Antonio N. Calabrese, Sheena E. Radford, Anastasia Zhuravleva

Publikováno v: Nature Communications, Vol 15, Iss 1, Pp 1-16 (2024)

Abstract The ATP-independent chaperone SurA protects unfolded outer membrane proteins (OMPs) from aggregation in the periplasm of Gram-negative bacteria, and delivers them to the β-barrel assembly machinery (BAM) for folding into the outer membrane

Externí odkaz: https://doaj.org/article/b8ce23fd6b774989b970d0af57f9265e

Dynamic action of the Sec machinery during initiation, protein translocation and termination

Autor: Tomas Fessl, Daniel Watkins, Peter Oatley, William John Allen, Robin Adam Corey, Jim Horne, Steve A Baldwin, Sheena E Radford, Ian Collinson, Roman Tuma

Publikováno v: eLife, Vol 7 (2018)

Protein translocation across cell membranes is a ubiquitous process required for protein secretion and membrane protein insertion. In bacteria, this is mostly mediated by the conserved SecYEG complex, driven through rounds of ATP hydrolysis by the cy

Externí odkaz: https://doaj.org/article/f35fb7d26f204ee7a792ed18827c7779

Dynamics of Membrane Proteins Monitored by Single-Molecule Fluorescence Across Multiple Timescales

Autor: Tomas, Fessl, Joel A, Crossley, Daniel, Watkins, Marek, Scholz, Matthew A, Watson, Tara, Sabir, Sheena E, Radford, Ian, Collinson, Roman, Tuma

Publikováno v: Methods in molecular biology (Clifton, N.J.). 2168

Single-molecule techniques provide insights into the heterogeneity and dynamics of ensembles and enable the extraction of mechanistic information that is complementary to high-resolution structural techniques. Here, we describe the application of sin

Externí odkaz: https://explore.openaire.eu/search/publication?articleId=pmid________::9898c0da55c828b4e6caa3aef8f6b578
https://pubmed.ncbi.nlm.nih.gov/33582997

Evaluation of the Stability of DNA i-Motifs in the Nuclei of Living Mammalian Cells

Autor: Jean-Louis Mergny, Lukáš Trantírek, Tomáš Loja, Silvie Foldynová-Trantírková, Simon Dzatko, Daniel Krafčík, Robert Hänsel-Hertsch, Tomas Fessl, Radovan Fiala, Michaela Krafčíková

Publikováno v: Angewandte Chemie International Edition
Angewandte Chemie (International Ed. in English)

C‐rich DNA has the capacity to form a tetra‐stranded structure known as an i‐motif. The i‐motifs within genomic DNA have been proposed to contribute to the regulation of DNA transcription. However, direct experimental evidence for the existen

Externí odkaz: https://explore.openaire.eu/search/publication?articleId=doi_dedup___::7b0b74dc87b1219cbf45cf72b6e2d8b9
https://doi.org/10.1002/anie.201712284

Monitoring DNA–Ligand Interactions in Living Human Cells Using NMR Spectroscopy

Autor: Michaela Krafčíková, Silvie Foldynová-Trantírková, Coralie Caron, Tomáš Loja, Tomas Fessl, Radovan Fiala, Simon Dzatko, Robert Hänsel-Hertsch, Jean-Louis Mergny, Marie-Paule Teulade-Fichou, Anton Granzhan, Lukáš Trantírek

Publikováno v: Journal of the American Chemical Society
Journal of the American Chemical Society, American Chemical Society, 2019, 141 (34), pp.13281-13285. ⟨10.1021/jacs.9b03031⟩

Studies on DNA-ligand interactions in the cellular environment are problematic due to the lack of suitable biophysical tools. To address this need, we developed an in-cell NMR-based approach for monitoring DNA-ligand interactions inside the nuclei of

Externí odkaz: https://explore.openaire.eu/search/publication?articleId=doi_dedup___::e008f7b85aa5288d8f80ff1eac16bb70
https://hal.archives-ouvertes.fr/hal-02344480/document