Bacteriophage Sf6 Tailspike Protein for Detection of Shigella flexneri Pathogens

Autor: Sonja Kunstmann, Tom Scheidt, Saskia Buchwald, Alexandra Helm, Laurence A. Mulard, Angelika Fruth, Stefanie Barbirz

Publikováno v: Viruses, Vol 10, Iss 8, p 431 (2018)

Bacteriophage research is gaining more importance due to increasing antibiotic resistance. However, for treatment with bacteriophages, diagnostics have to be improved. Bacteriophages carry adhesion proteins, which bind to the bacterial cell surface,

Externí odkaz: https://doaj.org/article/144180117d434dbf95e0f3dcf7caf80f

Kinetic fingerprints differentiate the mechanisms of action of anti-Aβ antibodies

Autor: Catherine K. Xu, Sean R. A. Devenish, Fang Qian, Samuel I. A. Cohen, Oskar Hansson, Thierry Bussiere, Georg Meisl, Sara Linse, Tiernan T. O'Malley, Katja Bernfur, Tuomas P. J. Knowles, Michele Vendruscolo, Eimantas Sileikis, Paul H. Weinreb, Tom Scheidt, Christopher M. Dobson, Martin Lundqvist

Publikováno v: Nature Structural & Molecular Biology. 27:1125-1133

The amyloid cascade hypothesis, according to which the self-assembly of amyloid-β peptide (Aβ) is a causative process in Alzheimer’s disease, has driven many therapeutic efforts for the past 20 years. Failures of clinical trials investigating Aβ

Externí odkaz: https://explore.openaire.eu/search/publication?articleId=doi_________::509d989428265cfa7f1a5b5d14c0adf4
https://doi.org/10.1038/s41594-020-0505-6

The binding of the small heat-shock protein αB-crystallin to fibrils of α-synuclein is driven by entropic forces

Autor: Tuomas P. J. Knowles, Samuel Ness, Jacqueline A. Carozza, Michele Vendruscolo, Georg Meisl, Christopher M. Dobson, Quentin Peter, Marta Castellana-Cruz, Justin L. P. Benesch, Olga Tkachenko, Francesco A. Aprile, Tom Scheidt, Carl C Kolbe, Therese W. Herling, Mathias M. J. Bellaiche, Paolo Arosio

Publikováno v: Proceedings of the National Academy of Sciences of the United States of America, 118 (38)
Proceedings of the National Academy of Sciences of the United States of America

Significance The formation of amyloid fibrils and toxic oligomeric species has been shown to be inhibited by their interactions with molecular chaperones, thus modulating monomer sequestration and toxicity in the context of neurodegenerative diseases

Externí odkaz: https://explore.openaire.eu/search/publication?articleId=doi_dedup___::cd548e5213dde477dbc1175952f16517
https://doi.org/10.1073/pnas.2108790118

Label-free protein analysis using liquid chromatography with gravimetric detection

Autor: Tuomas P. J. Knowles, Jerome Charmet, Tadas Kartanas, Tuuli A. Hakala, Zenon Toprakcioglu, Tom Scheidt, Aviad Levin

The detection and analysis of proteins in a label-free manner under native solution conditions is an increasingly important objective in analytical bioscience platform development. Common approaches to detect native proteins in solution often require

Externí odkaz: https://explore.openaire.eu/search/publication?articleId=doi_dedup___::82c28287337c4f700af80c69aa83b945
http://wrap.warwick.ac.uk/145904/1/WRAP-label-free-protein-analysis-using-liquid-chromatography-gravimetric-detection-Charmet-2020.pdf

Rational design of a conformation-specific antibody for the quantification of Aβ oligomers

Autor: Ryan Limbocker, Tom Scheidt, Francesco A. Aprile, Michele Vendruscolo, Patricia C. Salinas, Pietro Sormanni, Michele Perni, Tuomas P. J. Knowles, Johnny Habchi, Christopher M. Dobson, Tomas Sneideris, Shianne Chhangur, Gabriella T. Heller, Francesco Simone Ruggeri, Steven F. Lee, Marina Podpolny, Lisa-Maria Needham, Benedetta Mannini

Publikováno v: Proceedings of the National Academy of Sciences of the United States of America
Proceedings of the National Academy of Sciences of the United States of America, 117(24), 13509-13518
Proceedings of the National Academy of Sciences of the United States of America 117 (2020) 24

Significance The accurate quantification of the amounts of small oligomeric assemblies formed by the amyloid β (Aβ) peptide represents a major challenge in the Alzheimer’s field. There is therefore great interest in the development of methods to

Externí odkaz: https://explore.openaire.eu/search/publication?articleId=doi_dedup___::ec4f5eeca510afd76ac631c5b0205b5e
https://pubmed.ncbi.nlm.nih.gov/32493749