A β-barrel for oil transport through lipid membranes: Dynamic NMR structures of AlkL

Autor: Tobias Schubeis, Jan Stanek, Guido Pintacuda, Wojciech Kopec, Bert L. de Groot, Tanguy Le Marchand, Csaba Daday, Loren B. Andreas, Kumar Tekwani Movellan, Tom S. Schwarzer, Kathrin Castiglione

Publikováno v: Proceedings of the National Academy of Sciences of the United States of America
Proceedings of the National Academy of Sciences of the United States of America, 2020, 117 (35), pp.21014-21021. ⟨10.1073/pnas.2002598117⟩
Proceedings of the National Academy of Sciences of the United States of America, National Academy of Sciences, 2020, 117 (35), pp.21014-21021. ⟨10.1073/pnas.2002598117⟩
Proceedings of the National Academy of Sciences

Significance Here we show how AlkL, a minimalistic outer-membrane protein from oil-consuming bacteria, exploits dynamics of extracellular loops to channel substrate across the polysaccharide barrier and into the hydrophobic interior of the outer memb

Externí odkaz: https://explore.openaire.eu/search/publication?articleId=doi_dedup___::517098999904d74d7089d4baf2bce6aa
https://hal.science/hal-03089165/file/Schubeis_PNAS2020.pdf

Preparative refolding of small monomeric outer membrane proteins

Autor: Tom S. Schwarzer, Swati Krishnan, Maria Hermann, Friedrich C. Simmel, Kathrin Castiglione

Publikováno v: Protein Expression and Purification. 132:171-181

The outer membrane of gram-negative bacteria constitutes an important hurdle for the transport of hydrophobic molecules into the cell. Mass flux is often facilitated by various outer membrane proteins. These proteins are of biotechnological importanc

Externí odkaz: https://explore.openaire.eu/search/publication?articleId=doi_dedup___::ff9ac2810633006a8c7855a50f039ae2
https://doi.org/10.1016/j.pep.2017.01.012

Selective

Autor: Mukul G, Jain, Daniela, Lalli, Jan, Stanek, Chandrakala, Gowda, Satya, Prakash, Tom S, Schwarzer, Tobias, Schubeis, Kathrin, Castiglione, Loren B, Andreas, P K, Madhu, Guido, Pintacuda, Vipin, Agarwal

Publikováno v: The journal of physical chemistry letters. 8(11)

Very fast magic-angle spinning (MAS80 kHz) NMR combined with high-field magnets has enabled the acquisition of proton-detected spectra in fully protonated solid samples with sufficient resolution and sensitivity. One of the primary challenges in stru

Externí odkaz: https://explore.openaire.eu/search/publication?articleId=pmid________::9381e2e26f338de9e2b8082d87e32a72
https://pubmed.ncbi.nlm.nih.gov/28492324

Membrane functionalization of polymersomes: alleviating mass transport limitations by integrating multiple selective membrane transporters for the diffusion of chemically diverse molecules

Autor: Kathrin Castiglione, Ludwig Klermund, Guoshu Wang, Tom S. Schwarzer

Publikováno v: Nanotechnology. 29:44LT01

Recently, the interest in polymersomes as nanoreactors for synthetic applications has increased due to interesting proof-of-concept studies, indicating a versatile use of polymeric vesicles to compartmentalize complex reaction cascades. However, the

Externí odkaz: https://explore.openaire.eu/search/publication?articleId=doi_dedup___::a14086593a7a37e2b0303ba2ecd24d96
https://doi.org/10.1088/1361-6528/aadb7e