Zobrazeno 1 - 10
of 296
pro vyhledávání: '"Tom Misteli"'
Autor:
Adib Keikhosravi, Faisal Almansour, Christopher H. Bohrer, Nadezda A. Fursova, Krishnendu Guin, Varun Sood, Tom Misteli, Daniel R. Larson, Gianluca Pegoraro
Publikováno v:
Scientific Reports, Vol 14, Iss 1, Pp 1-16 (2024)
Abstract High-throughput imaging (HTI) generates complex imaging datasets from a large number of experimental perturbations. Commercial HTI software programs for image analysis workflows typically do not allow full customization and adoption of new i
Externí odkaz:
https://doaj.org/article/136cbb318d4f481fbe53b28ed81e7c7e
Publikováno v:
eLife, Vol 12 (2023)
The shape and size of the human cell nucleus is highly variable among cell types and tissues. Changes in nuclear morphology are associated with disease, including cancer, as well as with premature and normal aging. Despite the very fundamental nature
Externí odkaz:
https://doaj.org/article/e5b178f1765b46fe8d1547e242e578f4
Publikováno v:
Cell Reports, Vol 42, Iss 5, Pp 112534- (2023)
Summary: One of the major cellular mechanisms to ensure cellular protein homeostasis is the endoplasmic reticulum (ER) stress response. This pathway is triggered by accumulation of misfolded proteins in the ER lumen. The ER stress response is also ac
Externí odkaz:
https://doaj.org/article/2e6edf4b69434f418175323628943e45
Autor:
Kelsey Gallant, Arsun Bektas, Mary Kaileh, Ana Lustig, Luigi Ferrucci, Gianluca Pegoraro, Tom Misteli
Publikováno v:
Scientific Reports, Vol 12, Iss 1, Pp 1-13 (2022)
Abstract DNA damage is a prominent biomarker for numerous diseases, including cancer, as well as for the aging process. Detection of DNA damage routinely relies on traditional microscopy or cytometric methods. However, these techniques are typically
Externí odkaz:
https://doaj.org/article/83191778cc0142e08698fef978a85e5e
Autor:
Elizabeth H Finn, Tom Misteli
Publikováno v:
PLoS Genetics, Vol 18, Iss 10, p e1010451 (2022)
The human genome is non-randomly organized within the cell nucleus. Spatial mapping of genome folding by biochemical methods and imaging has revealed extensive variation in locus interaction frequencies between cells in a population and between homol
Externí odkaz:
https://doaj.org/article/e73918d9f0d8470f979e5b4181efcf50
Autor:
Yuto Takahashi, Shogo Hiratsuka, Nanako Machida, Daisuke Takahashi, Junpei Matsushita, Pavel Hozak, Tom Misteli, Kei Miyamoto, Masahiko Harata
Publikováno v:
Nucleus, Vol 11, Iss 1, Pp 250-263 (2020)
Hutchinson-Gilford progeria syndrome (HGPS) is a premature aging disorder caused by a mutation of lamin A, which contributes to nuclear architecture and the spatial organization of chromatin in the nucleus. The expression of a lamin A mutant, named p
Externí odkaz:
https://doaj.org/article/238cf4aa86e74a3696bc1c4fbe9b70ed
Autor:
Elizabeth H. Finn, Tom Misteli
Publikováno v:
STAR Protocols, Vol 2, Iss 3, Pp 100741- (2021)
Summary: Here, we describe an end-to-end high-throughput imaging protocol to visualize genomic loci in cells at high throughput using DNA fluorescence in situ hybridization, automated microscopy, and computational analysis. This is particularly usefu
Externí odkaz:
https://doaj.org/article/32bd8018c95e44e89aa2b6ccb06e1e8e
Autor:
Magda R Hamczyk, Ricardo Villa‐Bellosta, Víctor Quesada, Pilar Gonzalo, Sandra Vidak, Rosa M Nevado, María J Andrés‐Manzano, Tom Misteli, Carlos López‐Otín, Vicente Andrés
Publikováno v:
EMBO Molecular Medicine, Vol 11, Iss 4, Pp 1-13 (2019)
Abstract Hutchinson–Gilford progeria syndrome (HGPS) is a rare genetic disorder caused by progerin, a mutant lamin A variant. HGPS patients display accelerated aging and die prematurely, typically from atherosclerosis complications. Recently, we de
Externí odkaz:
https://doaj.org/article/90fa5e8960124fb793cbbc7fdd07bf46
Publikováno v:
BMC Bioinformatics, Vol 19, Iss 1, Pp 1-12 (2018)
Abstract Background Image-based high-throughput screening (HTS) reveals a high level of heterogeneity in single cells and multiple cellular states may be observed within a single population. Currently available high-dimensional analysis methods are s
Externí odkaz:
https://doaj.org/article/cc4e9232219342d89e8e2ef33729032e
Autor:
Karen J. Meaburn, Tom Misteli
Publikováno v:
Frontiers in Genetics, Vol 10 (2019)
There is a pressing need for additional clinical biomarkers to predict the aggressiveness of individual cancers. Here, we examine the potential usefulness of spatial genome organization as a prognostic tool for prostate cancer. Using fluorescence in
Externí odkaz:
https://doaj.org/article/940048d191544bc794be31de757eeae8