Zobrazeno 1 - 10
of 29
pro vyhledávání: '"Tom Lawrenson"'
Autor:
Tom Lawrenson, Martha Clarke, Rachel Kirby, Macarena Forner, Burkhard Steuernagel, James K. M. Brown, Wendy Harwood
Publikováno v:
Plant Methods, Vol 20, Iss 1, Pp 1-16 (2024)
Abstract Background CRISPR Cas9 and Cas12a are the two most frequently used programmable nucleases reported in plant systems. There is now a wide range of component parts for both which likely have varying degrees of effectiveness and potentially app
Externí odkaz:
https://doaj.org/article/d46511b0867f4a40ad9b6dabf861ddd7
Autor:
Fabio D’Orso, Lionel Hill, Ingo Appelhagen, Tom Lawrenson, Marco Possenti, Jie Li, Wendy Harwood, Giorgio Morelli, Cathie Martin
Publikováno v:
Frontiers in Plant Science, Vol 14 (2023)
The most abundant phenolic compound in Solanaceous plants is chlorogenic acid (CGA), which possesses protective properties such as antimicrobial and antioxidant activities. These properties are particularly relevant when plants are under adverse cond
Externí odkaz:
https://doaj.org/article/24b9b19ec648478091d9e6c835f613e4
Publikováno v:
Frontiers in Genome Editing, Vol 3 (2021)
Advances in the use of RNA-guided Cas9-based genome editing in plants have been rapid over the last few years. A desirable application of genome editing is gene targeting (GT), as it allows a wide range of precise modifications; however, this remains
Externí odkaz:
https://doaj.org/article/5c55657945464a528fd2245118a825e7
Publikováno v:
Methods in Molecular Biology ISBN: 9781071631300
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_________::db453af74489eaedc6b5175f6b78f9d3
https://doi.org/10.1007/978-1-0716-3131-7_16
https://doi.org/10.1007/978-1-0716-3131-7_16
Publikováno v:
Methods in Molecular Biology ISBN: 9781071631300
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_________::9cacb162067d99e469a651be397733ee
https://doi.org/10.1007/978-1-0716-3131-7_15
https://doi.org/10.1007/978-1-0716-3131-7_15
Novel versions of Cas12a, together with optimised guide architecture led to barley target genes being mutagenised in around 90% of transgenic lines. This system also functioned in Brassica oleracea.
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_________::f05440fb22a129276c2da7c48731d94c
https://doi.org/10.21203/rs.3.rs-2049530/v1
https://doi.org/10.21203/rs.3.rs-2049530/v1
Autor:
Mikhaela Neequaye, Richard Mithen, Penny Hundleby, Judith A. Irwin, Shikha Saha, Lars Østergaard, Sophia Stavnstrup, Wendy Harwood, Tom Lawrenson, Maria Traka, Perla Troncoso-Rey
Publikováno v:
The CRISPR Journal. 4:416-426
Discoveries in model plants grown under optimal conditions can provide important directions for crop improvement. However, it is important to verify whether results can be translated to crop plants grown in the field. In this study, we sought to stud
We report the first successful, high efficiency use of LbCas12a in barley and describe the development and application of two novel Cas12a variants. In total we compared five coding sequence (CDS) variants including the two novel ones and two guide a
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_________::b0d95859ef39ab1bf58f55ea181c8de2
https://doi.org/10.1101/2022.04.28.489853
https://doi.org/10.1101/2022.04.28.489853
We report highly efficient genome editing in Brassica species. We compare the efficiency of targeted mutagenesis using four Streptococcus pyogenes Cas9 (SpCas9) systems in Brassica oleracea and Brassica napus over 3 target genes and five guides to id
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_________::fd770c3bf2bebe394ee03e1025dd5fd0
https://doi.org/10.1101/2022.05.16.492057
https://doi.org/10.1101/2022.05.16.492057
Publikováno v:
Frontiers in Genome Editing
Frontiers in Genome Editing, Vol 3 (2021)
Frontiers in Genome Editing, Vol 3 (2021)
Advances in the use of RNA-guided Cas9-based genome editing in plants have been rapid over the last few years. A desirable application of genome editing is gene targeting (GT), as it allows a wide range of precise modifications; however, this remains
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_dedup___::6b9a62bccde2b942f8de2175155962a3
https://doi.org/10.1101/2021.02.04.429787
https://doi.org/10.1101/2021.02.04.429787