Výsledky vyhledávání - "Tom Kirchhausen"

Akademický článek

PKC-phosphorylation of Liprin-α3 triggers phase separation and controls presynaptic active zone structure

Autor: Javier Emperador-Melero, Man Yan Wong, Shan Shan H. Wang, Giovanni de Nola, Hajnalka Nyitrai, Tom Kirchhausen, Pascal S. Kaeser

Publikováno v: Nature Communications, Vol 12, Iss 1, Pp 1-17 (2021)

Liquid–liquid phase separation may be a mechanism for organizing the presynaptic nerve terminal. Here, the authors show that PKC-mediated phosphorylation of Liprin-α3 triggers phase separation in cell lines and modulates active zone structure and

Externí odkaz: https://doaj.org/article/15b0f79f4c614f568a9dd724cdd9d663

Zobrazit plný text záznamu

Akademický článek

Design and Validation of a Human Brain Endothelial Microvessel-on-a-Chip Open Microfluidic Model Enabling Advanced Optical Imaging

Autor: Mootaz M. Salman, Graham Marsh, Ilja Kusters, Matthieu Delincé, Giuseppe Di Caprio, Srigokul Upadhyayula, Giovanni de Nola, Ronan Hunt, Kazuka G. Ohashi, Taylor Gray, Fumitaka Shimizu, Yasuteru Sano, Takashi Kanda, Birgit Obermeier, Tom Kirchhausen

Publikováno v: Frontiers in Bioengineering and Biotechnology, Vol 8 (2020)

We describe here the design and implementation of an in vitro microvascular open model system using human brain microvascular endothelial cells. The design has several advantages over other traditional closed microfluidic platforms: (1) it enables co

Externí odkaz: https://doaj.org/article/a59750942c61446cad7b10bfa96a0dad

Zobrazit plný text záznamu

Akademický článek

Molecularly Distinct Clathrin-Coated Pits Differentially Impact EGFR Fate and Signaling

Publikováno v: Cell Reports, Vol 27, Iss 10, Pp 3049-3061.e6 (2019)

Summary: Adaptor protein 2 (AP2) is a major constituent of clathrin-coated pits (CCPs). Whether it is essential for all forms of clathrin-mediated endocytosis (CME) in mammalian cells is an open issue. Here, we demonstrate, by live TIRF microscopy, t

Externí odkaz: https://doaj.org/article/621ad877eaa14894b7e9f36e39fad5bd

Zobrazit plný text záznamu

Akademický článek

Data publication with the structural biology data grid supports live analysis

Autor: Peter A. Meyer, Stephanie Socias, Jason Key, Elizabeth Ransey, Emily C. Tjon, Alejandro Buschiazzo, Ming Lei, Chris Botka, James Withrow, David Neau, Kanagalaghatta Rajashankar, Karen S. Anderson, Richard H. Baxter, Stephen C. Blacklow, Titus J. Boggon, Alexandre M. J. J. Bonvin, Dominika Borek, Tom J. Brett, Amedeo Caflisch, Chung-I Chang, Walter J. Chazin, Kevin D. Corbett, Michael S. Cosgrove, Sean Crosson, Sirano Dhe-Paganon, Enrico Di Cera, Catherine L. Drennan, Michael J. Eck, Brandt F. Eichman, Qing R. Fan, Adrian R. Ferré-D'Amaré, J. Christopher Fromme, K. Christopher Garcia, Rachelle Gaudet, Peng Gong, Stephen C. Harrison, Ekaterina E. Heldwein, Zongchao Jia, Robert J. Keenan, Andrew C. Kruse, Marc Kvansakul, Jason S. McLellan, Yorgo Modis, Yunsun Nam, Zbyszek Otwinowski, Emil F. Pai, Pedro José Barbosa Pereira, Carlo Petosa, C. S. Raman, Tom A. Rapoport, Antonina Roll-Mecak, Michael K. Rosen, Gabby Rudenko, Joseph Schlessinger, Thomas U. Schwartz, Yousif Shamoo, Holger Sondermann, Yizhi J. Tao, Niraj H. Tolia, Oleg V. Tsodikov, Kenneth D. Westover, Hao Wu, Ian Foster, James S. Fraser, Filipe R. N C. Maia, Tamir Gonen, Tom Kirchhausen, Kay Diederichs, Mercè Crosas, Piotr Sliz

Publikováno v: Nature Communications, Vol 7, Iss 1, Pp 1-12 (2016)

The validation and analysis of X-ray crystallographic data is essential for reproducibility and the development of crystallographic methods. Here, the authors describe a repository for crystallographic datasets and demonstrate some of the ways it cou

Externí odkaz: https://doaj.org/article/72383da3679147f9a2668c703d709d2e

Zobrazit plný text záznamu

Akademický článek

Clathrin-Mediated Endocytosis Persists during Unperturbed Mitosis

Autor: Silvia K. Tacheva-Grigorova, António J.M. Santos, Emmanuel Boucrot, Tom Kirchhausen

Publikováno v: Cell Reports, Vol 4, Iss 4, Pp 659-668 (2013)

How does mitosis influence the critical process of endocytosis? Some experiments lead to the conclusion that endocytosis arrests completely during mitosis, whereas others indicate that endocytosis persists. We have resolved this apparent discrepancy

Externí odkaz: https://doaj.org/article/6df3f442844141318f3f4c6c4269531a

Zobrazit plný text záznamu

Akademický článek

Dynamics of Intracellular Clathrin/AP1- and Clathrin/AP3-Containing Carriers

Autor: Comert Kural, Silvia K. Tacheva-Grigorova, Steeve Boulant, Emanuele Cocucci, Thorsten Baust, Delfim Duarte, Tom Kirchhausen

Publikováno v: Cell Reports, Vol 2, Iss 5, Pp 1111-1119 (2012)

Clathrin/AP1- and clathrin/AP3-coated vesicular carriers originate from endosomes and the trans-Golgi network. Here, we report the real-time visualization of these structures in living cells reliably tracked by rapid, three-dimensional imaging with t

Externí odkaz: https://doaj.org/article/72550d8caf1a46a6bd33472b16991961

Zobrazit plný text záznamu

Akademický článek

Role of the clathrin adaptor PICALM in normal hematopoiesis and polycythemia vera pathophysiology

Autor: Yuichi Ishikawa, Manami Maeda, Mithun Pasham, Francois Aguet, Silvia K. Tacheva-Grigorova, Takeshi Masuda, Hai Yi, Sung-Uk Lee, Jian Xu, Julie Teruya-Feldstein, Maria Ericsson, Ann Mullally, John Heuser, Tom Kirchhausen, Takahiro Maeda

Publikováno v: Haematologica, Vol 100, Iss 4 (2015)

Clathrin-dependent endocytosis is an essential cellular process shared by all cell types. Despite this, precisely how endocytosis is regulated in a cell-type-specific manner and how this key pathway functions physiologically or pathophysiologically r

Externí odkaz: https://doaj.org/article/55fb15d1f76e46a9a89852204ed5decb

Zobrazit plný text záznamu

Vyhledávací nástroje:

Upřesnit hledání