Zobrazeno 1 - 10
of 72
pro vyhledávání: '"Tom Kirchhausen"'
Autor:
Javier Emperador-Melero, Man Yan Wong, Shan Shan H. Wang, Giovanni de Nola, Hajnalka Nyitrai, Tom Kirchhausen, Pascal S. Kaeser
Publikováno v:
Nature Communications, Vol 12, Iss 1, Pp 1-17 (2021)
Liquid–liquid phase separation may be a mechanism for organizing the presynaptic nerve terminal. Here, the authors show that PKC-mediated phosphorylation of Liprin-α3 triggers phase separation in cell lines and modulates active zone structure and
Externí odkaz:
https://doaj.org/article/15b0f79f4c614f568a9dd724cdd9d663
Autor:
Mootaz M. Salman, Graham Marsh, Ilja Kusters, Matthieu Delincé, Giuseppe Di Caprio, Srigokul Upadhyayula, Giovanni de Nola, Ronan Hunt, Kazuka G. Ohashi, Taylor Gray, Fumitaka Shimizu, Yasuteru Sano, Takashi Kanda, Birgit Obermeier, Tom Kirchhausen
Publikováno v:
Frontiers in Bioengineering and Biotechnology, Vol 8 (2020)
We describe here the design and implementation of an in vitro microvascular open model system using human brain microvascular endothelial cells. The design has several advantages over other traditional closed microfluidic platforms: (1) it enables co
Externí odkaz:
https://doaj.org/article/a59750942c61446cad7b10bfa96a0dad
Autor:
Roberta Pascolutti, Veronica Algisi, Alexia Conte, Andrea Raimondi, Mithun Pasham, Srigokul Upadhyayula, Raphael Gaudin, Tanja Maritzen, Elisa Barbieri, Giusi Caldieri, Chiara Tordonato, Stefano Confalonieri, Stefano Freddi, Maria Grazia Malabarba, Elena Maspero, Simona Polo, Carlo Tacchetti, Volker Haucke, Tom Kirchhausen, Pier Paolo Di Fiore, Sara Sigismund
Publikováno v:
Cell Reports, Vol 27, Iss 10, Pp 3049-3061.e6 (2019)
Summary: Adaptor protein 2 (AP2) is a major constituent of clathrin-coated pits (CCPs). Whether it is essential for all forms of clathrin-mediated endocytosis (CME) in mammalian cells is an open issue. Here, we demonstrate, by live TIRF microscopy, t
Externí odkaz:
https://doaj.org/article/621ad877eaa14894b7e9f36e39fad5bd
Autor:
Peter A. Meyer, Stephanie Socias, Jason Key, Elizabeth Ransey, Emily C. Tjon, Alejandro Buschiazzo, Ming Lei, Chris Botka, James Withrow, David Neau, Kanagalaghatta Rajashankar, Karen S. Anderson, Richard H. Baxter, Stephen C. Blacklow, Titus J. Boggon, Alexandre M. J. J. Bonvin, Dominika Borek, Tom J. Brett, Amedeo Caflisch, Chung-I Chang, Walter J. Chazin, Kevin D. Corbett, Michael S. Cosgrove, Sean Crosson, Sirano Dhe-Paganon, Enrico Di Cera, Catherine L. Drennan, Michael J. Eck, Brandt F. Eichman, Qing R. Fan, Adrian R. Ferré-D'Amaré, J. Christopher Fromme, K. Christopher Garcia, Rachelle Gaudet, Peng Gong, Stephen C. Harrison, Ekaterina E. Heldwein, Zongchao Jia, Robert J. Keenan, Andrew C. Kruse, Marc Kvansakul, Jason S. McLellan, Yorgo Modis, Yunsun Nam, Zbyszek Otwinowski, Emil F. Pai, Pedro José Barbosa Pereira, Carlo Petosa, C. S. Raman, Tom A. Rapoport, Antonina Roll-Mecak, Michael K. Rosen, Gabby Rudenko, Joseph Schlessinger, Thomas U. Schwartz, Yousif Shamoo, Holger Sondermann, Yizhi J. Tao, Niraj H. Tolia, Oleg V. Tsodikov, Kenneth D. Westover, Hao Wu, Ian Foster, James S. Fraser, Filipe R. N C. Maia, Tamir Gonen, Tom Kirchhausen, Kay Diederichs, Mercè Crosas, Piotr Sliz
Publikováno v:
Nature Communications, Vol 7, Iss 1, Pp 1-12 (2016)
The validation and analysis of X-ray crystallographic data is essential for reproducibility and the development of crystallographic methods. Here, the authors describe a repository for crystallographic datasets and demonstrate some of the ways it cou
Externí odkaz:
https://doaj.org/article/72383da3679147f9a2668c703d709d2e
Publikováno v:
Cell Reports, Vol 4, Iss 4, Pp 659-668 (2013)
How does mitosis influence the critical process of endocytosis? Some experiments lead to the conclusion that endocytosis arrests completely during mitosis, whereas others indicate that endocytosis persists. We have resolved this apparent discrepancy
Externí odkaz:
https://doaj.org/article/6df3f442844141318f3f4c6c4269531a
Autor:
Comert Kural, Silvia K. Tacheva-Grigorova, Steeve Boulant, Emanuele Cocucci, Thorsten Baust, Delfim Duarte, Tom Kirchhausen
Publikováno v:
Cell Reports, Vol 2, Iss 5, Pp 1111-1119 (2012)
Clathrin/AP1- and clathrin/AP3-coated vesicular carriers originate from endosomes and the trans-Golgi network. Here, we report the real-time visualization of these structures in living cells reliably tracked by rapid, three-dimensional imaging with t
Externí odkaz:
https://doaj.org/article/72550d8caf1a46a6bd33472b16991961
Autor:
Yuichi Ishikawa, Manami Maeda, Mithun Pasham, Francois Aguet, Silvia K. Tacheva-Grigorova, Takeshi Masuda, Hai Yi, Sung-Uk Lee, Jian Xu, Julie Teruya-Feldstein, Maria Ericsson, Ann Mullally, John Heuser, Tom Kirchhausen, Takahiro Maeda
Publikováno v:
Haematologica, Vol 100, Iss 4 (2015)
Clathrin-dependent endocytosis is an essential cellular process shared by all cell types. Despite this, precisely how endocytosis is regulated in a cell-type-specific manner and how this key pathway functions physiologically or pathophysiologically r
Externí odkaz:
https://doaj.org/article/55fb15d1f76e46a9a89852204ed5decb
Autor:
Alexandre P. Martin, Gary A. Bradshaw, Robyn J. Eisert, Emily D. Egan, Lena Tveriakhina, Julia M. Rogers, Andrew N. Dates, Gustavo Scanavachi, Jon C. Aster, Tom Kirchhausen, Marian Kalocsay, Stephen C. Blacklow
Notch signaling relies on ligand-induced proteolysis to liberate a nuclear effector that drives cell fate decisions. The location and timing of individual steps required for proteolysis and movement of Notch from membrane to nucleus, however, remain
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_________::72eec61f8f702d2a049806bff5c2a4d9
https://doi.org/10.1101/2022.12.21.521435
https://doi.org/10.1101/2022.12.21.521435
Autor:
Sai Luo, Jun Zhang, Alex J. B. Kreutzberger, Amanda Eaton, Robert J. Edwards, Changbin Jing, Hai-Qiang Dai, Gregory D. Sempowski, Kenneth Cronin, Robert Parks, Adam Yongxin Ye, Katayoun Mansouri, Maggie Barr, Novalia Pishesha, Aimee Chapdelaine Williams, Lucas Vieira Francisco, Anand Saminathan, Hanqin Peng, Himanshu Batra, Lorenza Bellusci, Surender Khurana, S. Munir Alam, David C. Montefiori, Kevin O. Saunders, Ming Tian, Hidde Ploegh, Tom Kirchhausen, Bing Chen, Barton F. Haynes, Frederick W. Alt
Publikováno v:
Science Immunology. 7
SARS-CoV-2 Omicron subvariants have generated a worldwide health crisis due to resistance to most approved SARS-CoV-2 neutralizing antibodies and evasion of vaccination-induced antibodies. To manage Omicron subvariants and prepare for new ones, addit
Autor:
Kailu Yang, Chuchu Wang, Alex J. B. Kreutzberger, Ravi Ojha, Suvi Kuivanen, Sergio Couoh-Cardel, Serena Muratcioglu, Timothy J. Eisen, K. Ian White, Richard G. Held, Subu Subramanian, Kendra Marcus, Richard A. Pfuetzner, Luis Esquivies, Catherine A. Doyle, John Kuriyan, Olli Vapalahti, Giuseppe Balistreri, Tom Kirchhausen, Axel T. Brunger
Publikováno v:
bioRxiv
Variants of severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) challenge currently available COVID-19 vaccines and monoclonal antibody therapies through epitope change on the receptor binding domain of the viral spike glycoprotein. Hence, t