Zobrazeno 1 - 10
of 33
pro vyhledávání: '"Tomás Aparicio"'
Publikováno v:
Microbial Biotechnology, Vol 14, Iss 6, Pp 2679-2685 (2021)
Summary Adaptive laboratory evolution (ALE) is a general and effective strategy for optimizing the design of engineered genetic circuits and upgrading metabolic phenotypes. However, the specific characteristics of each microorganism typically ask for
Externí odkaz:
https://doaj.org/article/0bbcc3bfade140428628e1b41f252434
Autor:
Coral García‐Gutiérrez, Tomás Aparicio, Lucía Torres‐Sánchez, Esteban Martínez‐García, Víctor deLorenzo, Claudio J. Villar, Felipe Lombó
Publikováno v:
MicrobiologyOpen, Vol 9, Iss 6, Pp 1135-1149 (2020)
Abstract Actinomycetales, such as the genus Streptomyces, are well‐known cell factories employed to produce a wide variety of secondary metabolites for industrial use. However, not only is the genetic engineering of Streptomyces more complicated an
Externí odkaz:
https://doaj.org/article/a61f33b6f6584d19bf2dfe096e44a25e
Propagation of Recombinant Genes through Complex Microbiomes with Synthetic Mini-RP4 Plasmid Vectors
Publikováno v:
BioDesign Research, Vol 2022 (2022)
The promiscuous conjugation machinery of the Gram-negative plasmid RP4 has been reassembled in a minimized, highly transmissible vector for propagating genetically encoded traits through diverse types of naturally occurring microbial communities. To
Externí odkaz:
https://doaj.org/article/7bf3be0e173b47b29e2be2a44e0324eb
Publikováno v:
Microbial Biotechnology, Vol 12, Iss 5, Pp 1076-1089 (2019)
Summary Implementation of single‐stranded DNA (ssDNA) recombineering in Pseudomonas putida has widened the range of genetic manipulations applicable to this biotechnologically relevant bacterium. Yet, the relatively low efficiency of the technology
Externí odkaz:
https://doaj.org/article/bc2fa6a5ddfb41dbbfc3573f7c9d0a6b
Publikováno v:
Good Microbes in Medicine, Food Production, Biotechnology, Bioremediation, and Agriculture. :325-336
Publikováno v:
In Energy Conversion and Management 15 January 2015 90:427-435
Autor:
Esteban Martínez-García, Sofía Fraile, Elena Algar, Tomás Aparicio, Elena Velázquez, Belén Calles, Huseyin Tas, Blas Blázquez, Bruno Martín, Clara Prieto, Lucas Sánchez-Sampedro, Morten H H Nørholm, Daniel C Volke, Nicolas T Wirth, Pavel Dvořák, Lorea Alejaldre, Lewis Grozinger, Matthew Crowther, Angel Goñi-Moreno, Pablo I Nikel, Juan Nogales, Víctor de Lorenzo
Publikováno v:
Martínez-García, E, Fraile, S, Algar, E, Aparicio, T, Velázquez, E, Calles, B, Tas, H, Blázquez, B, Martín, B, Prieto, C, Sánchez-Sampedro, L, Nørholm, M H H, Volke, D C, Wirth, N T, Dvořák, P, Alejaldre, L, Grozinger, L, Crowther, M, Goñi-Moreno, A, Nikel, P I, Nogales, J & de Lorenzo, V 2023, ' SEVA 4.0: an update of the Standard European Vector Architecture database for advanced analysis and programming of bacterial phenotypes ', Nucleic Acids Research, vol. 51, no. D1, pp. D1558–D1567 . https://doi.org/10.1093/nar/gkac1059
The SEVA platform (https://seva-plasmids.com) was launched one decade ago, both as a database (DB) and as a physical repository of plasmid vectors for genetic analysis and engineering of Gram-negative bacteria with a structure and nomenclature that f
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_dedup___::07f1b039e447131b2ad3fae697ca1df4
https://orbit.dtu.dk/en/publications/4e321df4-ea29-4104-8b3f-a08b9154a3a8
https://orbit.dtu.dk/en/publications/4e321df4-ea29-4104-8b3f-a08b9154a3a8
Publikováno v:
Methods in molecular biology (Clifton, N.J.). 2479
The ability to engineer bacterial genomes in an efficient way is crucial for many bio-related technologies. Single-stranded (ss) DNA recombineering technology allows to introduce mutations within bacterial genomes in a very simple and straightforward
Publikováno v:
ACS Synthetic Biology
Chromosomal exchange and subsequent recombination of the cognate DNA between bacteria was one of the most useful genetic tools (e.g., Hfr strains) for genetic analyses of E. coli before the genomic era. In this paper, yeast assembly has been used to
Publikováno v:
In IFAC Proceedings Volumes 2012 45(2):974-978