Zobrazeno 1 - 10
of 43
pro vyhledávání: '"Tomáš Gichner"'
Publikováno v:
Biologia plantarum. 58:783-787
We employed the comet assay (single cell gel electrophoresis) to evaluate induced DNA damage in nuclei isolated from tobacco leaves (Nicotiana tabacum var. xanthi) inoculated with Potato virus X (PVX). The highest DNA damage, expressed by the tail mo
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_________::5498be80f6e3c9be75b39b7a52275a42
https://doi.org/10.1007/s10535-014-0448-z
https://doi.org/10.1007/s10535-014-0448-z
Publikováno v:
Biologia plantarum. 57:783-787
We applied the alkaline version of the single-cell gel electrophoresis (comet assay) to seedlings of heterozygous tobacco (Nicotiana tabacum L. var. xanthi) treated with zinc acetate dihydrate (20 to 80 mM Zn2+ for 2 h or 2 to 12 mM Zn2+ for 24 h). A
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_________::c9445bbd28aa828ec3d3f5b551c888c7
https://doi.org/10.1007/s10535-013-0345-x
https://doi.org/10.1007/s10535-013-0345-x
Publikováno v:
The Comet Assay in Toxicology ISBN: 9781782622871
The Comet Assay in Toxicology ISBN: 9780854041992
The Comet Assay in Toxicology ISBN: 9780854041992
Plants are exposed to many environmental pollutants that are globally dispersed through aerial or aqueous pathways. In addition, agronomic crops as well as other plants are deliberately exposed to pesticides and other chemicals applied in modern agri
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_dedup___::a488f094079877bf549f4b1265a6e059
https://doi.org/10.1039/9781782622895-00112
https://doi.org/10.1039/9781782622895-00112
Publikováno v:
Environmental and Experimental Botany. 62:113-119
We have calibrated the alkaline protocol of the plant comet (Single Cell Gel Electrophoresis) assay as a method for detecting the extent of induced DNA damage in potato plants (Solanum tuberosum L. cultivar Korela). After 2 and 24 h treatments of the
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_________::a710559f19d4f08cd15c2adb3315482f
https://doi.org/10.1016/j.envexpbot.2007.07.013
https://doi.org/10.1016/j.envexpbot.2007.07.013
Publikováno v:
Mutation Research/Genetic Toxicology and Environmental Mutagenesis. 605:17-21
We applied the alkaline version of the single-cell gel electrophoresis (comet) assay to roots and leaves of tobacco (Nicotiana tabacum var. xanthi) seedlings or isolated leaf nuclei treated with: (1) the alkylating agent ethyl methanesulphonate, (2)
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_dedup___::f02d57f04c55e72322cda17ebc1bfe66
https://doi.org/10.1016/j.mrgentox.2006.01.005
https://doi.org/10.1016/j.mrgentox.2006.01.005
Publikováno v:
Biologia plantarum. 46:185-188
For most crops growing in polluted areas or treated with agricultural chemicals, no genotoxicity assays are available. We have studied the possibility of using the alkaline protocol of the plant-based molecular assay - the Single Cell Gel Electrophor
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_________::0459129b39b595d03a4239eb9bf5df5a
https://doi.org/10.1023/b:biop.0000022249.86426.2a
https://doi.org/10.1023/b:biop.0000022249.86426.2a
Autor:
Tomáš Gichner
Publikováno v:
Mutation Research/Genetic Toxicology and Environmental Mutagenesis. 535:187-193
We have measured the level of DNA damage induced by treating roots (cellular Comet assay) and isolated root nuclei (acellular Comet assay) of catalase-deficient (CAT1AS) and wild-type (SR1) tobacco with the promutagen o -phenylenediamine ( o -PDA) an
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_________::c1bf81d7f56ef95ba662fc2411080caa
https://doi.org/10.1016/s1383-5718(02)00320-0
https://doi.org/10.1016/s1383-5718(02)00320-0
Autor:
Z. Mühlfeldová, Tomáš Gichner
Publikováno v:
Biologia plantarum. 45:509-516
For most plant species growing in polluted areas no mutagenicity assays are available. We have studied the possibility of using the alkaline protocol of the Comet assay as a method for detecting induced DNA damage in wildly growing weeds. The monofuc
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_________::34600aa25220b55de1ea5f7fe16ce787
https://doi.org/10.1023/a:1022360722422
https://doi.org/10.1023/a:1022360722422
Publikováno v:
Mutation Research/Genetic Toxicology and Environmental Mutagenesis. 491:17-23
The advantage of using the tobacco (Nicotiana tabacum var. xanthi) mutagenicity assay is the ability to analyze and compare on the same plants under identical treatment conditions both the induced acute DNA damage in somatic cells as measured by the
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_dedup___::af244affa9110ca67d48695265c4708f
https://doi.org/10.1016/s1383-5718(00)00146-7
https://doi.org/10.1016/s1383-5718(00)00146-7
Publikováno v:
Environmental and Molecular Mutagenesis. 33:279-286
The use of single cell gel electrophoresis (SCGE) has recently been applied to plant systems. We optimized the experimental conditions for SCGE analysis using nuclei isolated from different tissues of intact plants. Concentration-response curves of g
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_________::b78019aadb90b681a85144aa2fe3b132
https://doi.org/10.1002/(sici)1098-2280(1999)33:4<279::aid-em4>3.0.co
https://doi.org/10.1002/(sici)1098-2280(1999)33:4<279::aid-em4>3.0.co