Zobrazeno 1 - 10
of 257
pro vyhledávání: '"Tohru Koike"'
Publikováno v:
Journal of Chromatography Open, Vol 2, Iss , Pp 100051- (2022)
For phosphoproteomics, it is important to separate and enrich phosphoproteins from complex biological samples predominantly containing their nonphosphorylated counterparts. This review describes effective chromatographic techniques for the separation
Externí odkaz:
https://doaj.org/article/3cfef66d6b8e44afb91ebb5e67746bc4
Publikováno v:
IUCrData, Vol 7, Iss 8, p x220854 (2022)
The two ZnII atoms in the crystal structure of the title complex, [Zn(NO3)2(C10H24N4)]·CH3OH, have a distorted octahedral coordination sphere, defined by 1,4,8,11-tetraazacyclotetradecane (cyclam) N atoms in the equatorial plane and nitrate O atoms
Externí odkaz:
https://doaj.org/article/915284bb06184c0ba8d2d6dfa407f09a
Publikováno v:
BioTechniques, Vol 70, Iss 4, Pp 209-217 (2021)
ABL, a human tyrosine protein kinase, and its substrate are co-expressed in Escherichia coli. Tyrosine phosphorylation of the substrate in E. coli was detected using Phos-tag SDS-PAGE. The bacterial co-expression system was used as a field for the ki
Externí odkaz:
https://doaj.org/article/90fb2a3a7ede436ca2d5a9a2e35dfced
Autor:
Emiko Kinoshita-Kikuta, Toshihiko Utsumi, Aya Miyazaki, Chiharu Tokumoto, Kyosuke Doi, Haruna Harada, Eiji Kinoshita, Tohru Koike
Publikováno v:
Scientific Reports, Vol 10, Iss 1, Pp 1-13 (2020)
Abstract Protein N-myristoylation of Src-family kinases (SFKs) is a critical co-translational modification to anchor the enzymes in the plasma membrane. Phosphorylation of SFKs is also an essential modification for regulating their enzymatic activiti
Externí odkaz:
https://doaj.org/article/1ec01883bf834b3e907739d568b11fa8
Autor:
Yoshimi Ichimaru, Koichi Kato, Hiromasa Kurosaki, Haruto Fujioka, Misa Sakai, Yoshihiro Yamaguchi, Jin Wanchun, Kirara Sugiura, Masanori Imai, Tohru Koike
Publikováno v:
IUCrData, Vol 6, Iss 4, p x210397 (2021)
The cationic ZnII part of aqua(1,4,7,10-tetraazacyclododecane)zinc(II) bis(perchlorate), [Zn(C8H20N4)(H2O)](ClO4)2, exhibits a slightly distorted square-pyramidal coordination environment with a water molecule in the apical position. In the crystal,
Externí odkaz:
https://doaj.org/article/357466873f754506a7f1623824853f22
Autor:
Emiko Kinoshita-Kikuta, Eiji Kinoshita, Misaki Suga, Mana Higashida, Yuka Yamane, Tomoka Nakamura, Tohru Koike
Publikováno v:
Biomolecules, Vol 11, Iss 10, p 1448 (2021)
The production of heterologous proteins is an important procedure for biologists in basic and applied sciences. A variety of cell-based and cell-free protein expression systems are available to achieve this. The expression system must be selected car
Externí odkaz:
https://doaj.org/article/18296019fd5640729820a777454d6675
Autor:
Emiko Kinoshita-Kikuta, Ayane Tanikawa, Takuro Hosokawa, Aya Kiwado, Koko Moriya, Eiji Kinoshita, Tohru Koike, Toshihiko Utsumi
Publikováno v:
PLoS ONE, Vol 14, Iss 11, p e0225510 (2019)
To establish a strategy for identifying protein-N-myristoylation-dependent phosphorylation of cellular proteins, Phos-tag SDS-PAGE was performed on wild-type (WT) and nonmyristoylated mutant (G2A-mutant) FMNL2 and FMNL3, phosphorylated N-myristoylate
Externí odkaz:
https://doaj.org/article/0dbd784a231e43d1bdb8e5f0c6b31472
Publikováno v:
Proteomes, Vol 3, Iss 1, Pp 42-55 (2015)
Affinity electrophoresis is an important technique that is widely used to separate and analyze biomolecules in the fields of biology and medicine. Both quantitative and qualitative information can be gained through affinity electrophoresis. Affinity
Externí odkaz:
https://doaj.org/article/dc627a77549842a3ad52b241ef794873
Autor:
Yasunori Sugiyama, Syouichi Katayama, Isamu Kameshita, Keiko Morisawa, Takuma Higuchi, Hiroshi Todaka, Eiji Kinoshita, Emiko Kinoshita-Kikuta, Tohru Koike, Taketoshi Taniguchi, Shuji Sakamoto
Publikováno v:
MethodsX, Vol 2, Iss C, Pp 469-474 (2015)
Protein kinase expression and activity play important roles in diverse cellular functions through regulation of phosphorylation signaling. The most commonly used tools for detecting the protein kinase are protein kinase-specific antibodies, and phosp
Externí odkaz:
https://doaj.org/article/1d4949930c884dbfa0f3a4a90c32f4de
Publikováno v:
PLoS ONE, Vol 11, Iss 2, p e0148294 (2016)
Tripartite sensor kinases (TSKs) have three phosphorylation sites on His, Asp, and His residues, which are conserved in a histidine kinase (HK) domain, a receiver domain, and a histidine-containing phosphotransmitter (HPt) domain, respectively. By me
Externí odkaz:
https://doaj.org/article/92b61a9335324a6299ff8f07138a76d5