Fluorescent tagging of endogenous IRS2 with an auxin-dependent degron to assess dynamic intracellular localization and function.

Autor: Jo M; Department of Molecular, Cell & Cancer Biology, University of Massachusetts Chan Medical School, Worcester, Massachusetts, USA., Lee JS; Department of Molecular, Cell & Cancer Biology, University of Massachusetts Chan Medical School, Worcester, Massachusetts, USA., Tocheny CE; Department of Molecular, Cell & Cancer Biology, University of Massachusetts Chan Medical School, Worcester, Massachusetts, USA., Lero MW; Department of Molecular, Cell & Cancer Biology, University of Massachusetts Chan Medical School, Worcester, Massachusetts, USA., Bui QT; Department of Molecular, Cell & Cancer Biology, University of Massachusetts Chan Medical School, Worcester, Massachusetts, USA., Morgan JS; Department of Molecular, Cell & Cancer Biology, University of Massachusetts Chan Medical School, Worcester, Massachusetts, USA., Shaw LM; Department of Molecular, Cell & Cancer Biology, University of Massachusetts Chan Medical School, Worcester, Massachusetts, USA. Electronic address: leslie.shaw@umassmed.edu.

Publikováno v: The Journal of biological chemistry [J Biol Chem] 2024 Sep 19; Vol. 300 (11), pp. 107796. Date of Electronic Publication: 2024 Sep 19.

The insulin and IGF signaling pathway sustains breast cancer stem cells by IRS2/PI3K-mediated regulation of MYC.

Autor: Lee JS; Department of Molecular, Cell & Cancer Biology, University of Massachusetts Chan Medical School, 364 Plantation St., Worcester, MA 01605, USA., Lero MW; Department of Molecular, Cell & Cancer Biology, University of Massachusetts Chan Medical School, 364 Plantation St., Worcester, MA 01605, USA., Mercado-Matos J; Department of Molecular, Cell & Cancer Biology, University of Massachusetts Chan Medical School, 364 Plantation St., Worcester, MA 01605, USA., Zhu S; Department of Molecular, Cell & Cancer Biology, University of Massachusetts Chan Medical School, 364 Plantation St., Worcester, MA 01605, USA., Jo M; Department of Molecular, Cell & Cancer Biology, University of Massachusetts Chan Medical School, 364 Plantation St., Worcester, MA 01605, USA., Tocheny CE; Department of Molecular, Cell & Cancer Biology, University of Massachusetts Chan Medical School, 364 Plantation St., Worcester, MA 01605, USA., Morgan JS; Department of Molecular, Cell & Cancer Biology, University of Massachusetts Chan Medical School, 364 Plantation St., Worcester, MA 01605, USA., Shaw LM; Department of Molecular, Cell & Cancer Biology, University of Massachusetts Chan Medical School, 364 Plantation St., Worcester, MA 01605, USA. Electronic address: leslie.shaw@umassmed.edu.

Publikováno v: Cell reports [Cell Rep] 2022 Dec 06; Vol. 41 (10), pp. 111759.

Rapid GPR183-mediated recruitment of eosinophils to the lung after Mycobacterium tuberculosis infection.

Autor: Bohrer AC; Inflammation and Innate Immunity Unit, Laboratory of Clinical Immunology and Microbiology, National Institute of Allergy and Infectious Diseases (NIAID), National Institutes of Health (NIH), Bethesda, MD 20892, USA., Castro E; Inflammation and Innate Immunity Unit, Laboratory of Clinical Immunology and Microbiology, National Institute of Allergy and Infectious Diseases (NIAID), National Institutes of Health (NIH), Bethesda, MD 20892, USA., Tocheny CE; Inflammation and Innate Immunity Unit, Laboratory of Clinical Immunology and Microbiology, National Institute of Allergy and Infectious Diseases (NIAID), National Institutes of Health (NIH), Bethesda, MD 20892, USA., Assmann M; Inflammation and Innate Immunity Unit, Laboratory of Clinical Immunology and Microbiology, National Institute of Allergy and Infectious Diseases (NIAID), National Institutes of Health (NIH), Bethesda, MD 20892, USA., Schwarz B; Immunity to Pulmonary Pathogens Section, Laboratory of Bacteriology, National Institute of Allergy and Infectious Diseases (NIAID), National Institutes of Health (NIH), Hamilton, MT 59840, USA., Bohrnsen E; Immunity to Pulmonary Pathogens Section, Laboratory of Bacteriology, National Institute of Allergy and Infectious Diseases (NIAID), National Institutes of Health (NIH), Hamilton, MT 59840, USA., Makiya MA; Human Eosinophil Section, Laboratory of Parasitic Diseases, NIAID, NIH, Bethesda, MD 20892, USA., Legrand F; Human Eosinophil Section, Laboratory of Parasitic Diseases, NIAID, NIH, Bethesda, MD 20892, USA., Hilligan KL; Immunobiology Section, Laboratory of Parasitic Diseases, NIAID, NIH, Bethesda, MD 20892, USA., Baker PJ; Inflammation and Innate Immunity Unit, Laboratory of Clinical Immunology and Microbiology, National Institute of Allergy and Infectious Diseases (NIAID), National Institutes of Health (NIH), Bethesda, MD 20892, USA., Torres-Juarez F; Inflammation and Innate Immunity Unit, Laboratory of Clinical Immunology and Microbiology, National Institute of Allergy and Infectious Diseases (NIAID), National Institutes of Health (NIH), Bethesda, MD 20892, USA., Hu Z; Department of Scientific Research, Shanghai Public Health Clinical Center (SPHCC), Fudan University, Shanghai 201508, China; TB Center, Shanghai Emerging and Re-Emerging Infectious Disease Institute, Fudan University, Shanghai 201508, China., Ma H; Department of Scientific Research, Shanghai Public Health Clinical Center (SPHCC), Fudan University, Shanghai 201508, China; TB Center, Shanghai Emerging and Re-Emerging Infectious Disease Institute, Fudan University, Shanghai 201508, China., Wang L; TB Center, Shanghai Emerging and Re-Emerging Infectious Disease Institute, Fudan University, Shanghai 201508, China; Department of Thoracic Surgery, Shanghai Public Health Clinical Center (SPHCC), Fudan University, Shanghai 201508, China., Niu L; Department of Scientific Research, Shanghai Public Health Clinical Center (SPHCC), Fudan University, Shanghai 201508, China; TB Center, Shanghai Emerging and Re-Emerging Infectious Disease Institute, Fudan University, Shanghai 201508, China., Wen Z; TB Center, Shanghai Emerging and Re-Emerging Infectious Disease Institute, Fudan University, Shanghai 201508, China; Department of Thoracic Surgery, Shanghai Public Health Clinical Center (SPHCC), Fudan University, Shanghai 201508, China., Lee SH; Intracellular Parasite Biology Section, Laboratory of Parasitic Diseases, NIAID, NIH, Bethesda, MD 20892, USA., Kamenyeva O; Biological Imaging Section, Research Technologies Branch, National Institute of Allergy and Infectious Diseases (NIAID), National Institutes of Health (NIH), Bethesda, MD 20892, USA., Kauffman KD; T Lymphocyte Biology Section, Laboratory of Parasitic Disease, National Institute of Allergy and Infectious Diseases (NIAID), National Institutes of Health (NIH), Bethesda, MD 20892, USA., Donato M; Institute for Immunity, Transplantation and Infection, School of Medicine, Stanford University, Stanford, CA 94305, USA; Center for Biomedical Informatics Research, Department of Medicine, Stanford University, Stanford, CA 94305, USA., Sher A; Immunobiology Section, Laboratory of Parasitic Diseases, NIAID, NIH, Bethesda, MD 20892, USA., Barber DL; T Lymphocyte Biology Section, Laboratory of Parasitic Disease, National Institute of Allergy and Infectious Diseases (NIAID), National Institutes of Health (NIH), Bethesda, MD 20892, USA., Via LE; Tuberculosis Imaging Program (TBIP), Division of Intramural Research, National Institute of Allergy and Infectious Diseases (NIAID), National Institutes of Health (NIH), Bethesda, MD 20892, USA; Tuberculosis Research Section, LCIM, NIAID, NIH, Bethesda, MD 20892, USA., Scriba TJ; South African Tuberculosis Vaccine Initiative, Institute of Infectious Disease & Molecular Medicine and Division of Immunology, Department of Pathology, University of Cape Town, Observatory, 7925, South Africa., Khatri P; Institute for Immunity, Transplantation and Infection, School of Medicine, Stanford University, Stanford, CA 94305, USA; Center for Biomedical Informatics Research, Department of Medicine, Stanford University, Stanford, CA 94305, USA., Song Y; TB Center, Shanghai Emerging and Re-Emerging Infectious Disease Institute, Fudan University, Shanghai 201508, China; Department of Thoracic Surgery, Shanghai Public Health Clinical Center (SPHCC), Fudan University, Shanghai 201508, China., Wong KW; Department of Scientific Research, Shanghai Public Health Clinical Center (SPHCC), Fudan University, Shanghai 201508, China; TB Center, Shanghai Emerging and Re-Emerging Infectious Disease Institute, Fudan University, Shanghai 201508, China., Bosio CM; Immunity to Pulmonary Pathogens Section, Laboratory of Bacteriology, National Institute of Allergy and Infectious Diseases (NIAID), National Institutes of Health (NIH), Hamilton, MT 59840, USA., Klion AD; Human Eosinophil Section, Laboratory of Parasitic Diseases, NIAID, NIH, Bethesda, MD 20892, USA., Mayer-Barber KD; Inflammation and Innate Immunity Unit, Laboratory of Clinical Immunology and Microbiology, National Institute of Allergy and Infectious Diseases (NIAID), National Institutes of Health (NIH), Bethesda, MD 20892, USA. Electronic address: mayerk@niaid.nih.gov.

Publikováno v: Cell reports [Cell Rep] 2022 Jul 26; Vol. 40 (4), pp. 111144.

Eosinophils are part of the granulocyte response in tuberculosis and promote host resistance in mice.

Autor: Bohrer AC; Inflammation and Innate Immunity Unit, Laboratory of Clinical Immunology and Microbiology, National Institute of Allergy and Infectious Diseases, National Institutes of Health, Bethesda, MD., Castro E; Inflammation and Innate Immunity Unit, Laboratory of Clinical Immunology and Microbiology, National Institute of Allergy and Infectious Diseases, National Institutes of Health, Bethesda, MD., Hu Z; Department of Scientific Research, Shanghai Public Health Clinical Center, Fudan University, Shanghai, China.; Tuberculosis Center, Shanghai Emerging and Re-emerging Infectious Disease Institute, Fudan University, Shanghai, China., Queiroz ATL; The KAB group, Multinational Organization Network Sponsoring Translational and Epidemiological Research Initiative, Instituto Gonçalo Moniz, Fundação Oswaldo Cruz, Salvador Brazil., Tocheny CE; Inflammation and Innate Immunity Unit, Laboratory of Clinical Immunology and Microbiology, National Institute of Allergy and Infectious Diseases, National Institutes of Health, Bethesda, MD., Assmann M; Inflammation and Innate Immunity Unit, Laboratory of Clinical Immunology and Microbiology, National Institute of Allergy and Infectious Diseases, National Institutes of Health, Bethesda, MD., Sakai S; T Lymphocyte Biology Section, Laboratory of Parasitic Diseases, National Institute of Allergy and Infectious Diseases, National Institutes of Health, Bethesda, MD., Nelson C; T Lymphocyte Biology Section, Laboratory of Parasitic Diseases, National Institute of Allergy and Infectious Diseases, National Institutes of Health, Bethesda, MD., Baker PJ; Inflammation and Innate Immunity Unit, Laboratory of Clinical Immunology and Microbiology, National Institute of Allergy and Infectious Diseases, National Institutes of Health, Bethesda, MD., Ma H; Department of Scientific Research, Shanghai Public Health Clinical Center, Fudan University, Shanghai, China.; Tuberculosis Center, Shanghai Emerging and Re-emerging Infectious Disease Institute, Fudan University, Shanghai, China., Wang L; Tuberculosis Center, Shanghai Emerging and Re-emerging Infectious Disease Institute, Fudan University, Shanghai, China.; Department of Thoracic Surgery, Shanghai Public Health Clinical Center, Fudan University, Shanghai, China., Zilu W; Tuberculosis Center, Shanghai Emerging and Re-emerging Infectious Disease Institute, Fudan University, Shanghai, China.; Department of Thoracic Surgery, Shanghai Public Health Clinical Center, Fudan University, Shanghai, China., du Bruyn E; Wellcome Centre for Infectious Diseases Research in Africa, Institute of Infectious Disease and Molecular Medicine, University of Cape Town, South Africa., Riou C; Wellcome Centre for Infectious Diseases Research in Africa, Institute of Infectious Disease and Molecular Medicine, University of Cape Town, South Africa., Kauffman KD; T Lymphocyte Biology Section, Laboratory of Parasitic Diseases, National Institute of Allergy and Infectious Diseases, National Institutes of Health, Bethesda, MD., Moore IN; Infectious Disease Pathogenesis Section, Comparative Medicine Branch, National Institute of Allergy and Infectious Diseases, National Institutes of Health, Bethesda, MD., Del Nonno F; Pathology Unit, National Institute for Infectious Diseases 'L. Spallanzani,' Istituto Di Ricovero e Cura a Carattere Scientifico, Rome, Italy., Petrone L; Translational Research Unit, Department of Epidemiology and Preclinical Research National Institute for Infectious Diseases, Istituto Di Ricovero e Cura a Carattere Scientifico, Rome, Italy., Goletti D; Translational Research Unit, Department of Epidemiology and Preclinical Research National Institute for Infectious Diseases, Istituto Di Ricovero e Cura a Carattere Scientifico, Rome, Italy., Martineau AR; Institute of Immunity and Transplantation, University College London, London, UK., Lowe DM; Institute of Immunity and Transplantation, University College London, London, UK., Cronan MR; In Vivo Cell Biology of Infection Unit, Max Planck Institute for Infection Biology, Berlin, Germany.; Department of Molecular Genetics and Microbiology, Duke University School of Medicine, Durham, NC., Wilkinson RJ; Wellcome Centre for Infectious Diseases Research in Africa, Institute of Infectious Disease and Molecular Medicine, University of Cape Town, South Africa.; Department of Infectious Diseases, Imperial College London, UK.; Francis Crick Institute, London, UK., Barry CE; Wellcome Centre for Infectious Diseases Research in Africa, Institute of Infectious Disease and Molecular Medicine, University of Cape Town, South Africa.; Tuberculosis Research Section, Laboratory of Clinical Immunology and Microbiology, National Institute of Allergy and Infectious Diseases, National Institutes of Health, Bethesda, MD., Via LE; Tuberculosis Imaging Program, Division of Intramural Research, National Institute of Allergy and Infectious Diseases, National Institutes of Health, Bethesda, MD.; Tuberculosis Research Section, Laboratory of Clinical Immunology and Microbiology, National Institute of Allergy and Infectious Diseases, National Institutes of Health, Bethesda, MD., Barber DL; T Lymphocyte Biology Section, Laboratory of Parasitic Diseases, National Institute of Allergy and Infectious Diseases, National Institutes of Health, Bethesda, MD., Klion AD; Human Eosinophil Section, Laboratory of Parasitic Diseases, National Institute of Allergy and Infectious Diseases, National Institutes of Health, Bethesda, MD., Andrade BB; The KAB group, Multinational Organization Network Sponsoring Translational and Epidemiological Research Initiative, Instituto Gonçalo Moniz, Fundação Oswaldo Cruz, Salvador Brazil., Song Y; Tuberculosis Center, Shanghai Emerging and Re-emerging Infectious Disease Institute, Fudan University, Shanghai, China.; Department of Thoracic Surgery, Shanghai Public Health Clinical Center, Fudan University, Shanghai, China., Wong KW; Department of Scientific Research, Shanghai Public Health Clinical Center, Fudan University, Shanghai, China.; Tuberculosis Center, Shanghai Emerging and Re-emerging Infectious Disease Institute, Fudan University, Shanghai, China., Mayer-Barber KD; Inflammation and Innate Immunity Unit, Laboratory of Clinical Immunology and Microbiology, National Institute of Allergy and Infectious Diseases, National Institutes of Health, Bethesda, MD.

Publikováno v: The Journal of experimental medicine [J Exp Med] 2021 Oct 04; Vol. 218 (10). Date of Electronic Publication: 2021 Aug 04.