Zobrazeno 1 - 10
of 22
pro vyhledávání: '"Tobias W. B. Ost"'
Autor:
Nicolas J. Walker, Mamunur Rashid, Shirong Yu, Helen Bignell, Casper K. Lumby, Carmen M. Livi, Kate Howell, David J. Morley, Sandro Morganella, Daniel Barrell, Shabhonam Caim, Walraj Gosal, Jens Füllgrabe, Thomas J. Charlesworth, Louella Vasquez, Miika Ahdesmäki, Jordan Eizenga, Parul Prabhat, Vitali Proutski, Marie Laurie Murat-Onana, Catherine J. Greenwood, Lisa Kirkwood, Meeta Maisuria-Armer, Mengjie Li, Emma Coats, Victoria Winfield, Lachlan MacBean, Toby Stock, Alice Tomé-Fernandez, Yat Chan, Nasir Sheikh, Paula Golder, Michael Steward, Tobias W. B. Ost, Douglas Stewart, Albert Vilella, Mojtaba Noursalehi, Benedict Paten, Debora Lucarelli, Joanne Mason, Gareth Ridge, Jason Mellad, Suman Shirodkar, Shankar Balasubaramanian, Joanna D. Holbrook
Publikováno v:
Scientific Reports, Vol 12, Iss 1, Pp 1-18 (2022)
Abstract Early detection of cancer will improve survival rates. The blood biomarker 5-hydroxymethylcytosine has been shown to discriminate cancer. In a large covariate-controlled study of over two thousand individual blood samples, we created, tested
Externí odkaz:
https://doaj.org/article/d0f8987336da4809bf0d43cd15a3ea02
Autor:
Jens Füllgrabe, Walraj S. Gosal, Páidí Creed, Sidong Liu, Casper K. Lumby, David J. Morley, Tobias W. B. Ost, Albert J. Vilella, Shirong Yu, Helen Bignell, Philippa Burns, Tom Charlesworth, Beiyuan Fu, Howerd Fordham, Nicolas J. Harding, Olga Gandelman, Paula Golder, Christopher Hodson, Mengjie Li, Marjana Lila, Yang Liu, Joanne Mason, Jason Mellad, Jack M. Monahan, Oliver Nentwich, Alexandra Palmer, Michael Steward, Minna Taipale, Audrey Vandomme, Rita Santo San-Bento, Ankita Singhal, Julia Vivian, Natalia Wójtowicz, Nathan Williams, Nicolas J. Walker, Nicola C. H. Wong, Gary N. Yalloway, Joanna D. Holbrook, Shankar Balasubramanian
Publikováno v:
Nature Biotechnology.
DNA comprises molecular information stored in genetic and epigenetic bases, both of which are vital to our understanding of biology. Most DNA sequencing approaches address either genetics or epigenetics and thus capture incomplete information. Method
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_________::9d8005a9ad5fe3c2a23faf8b6338fb23
https://doi.org/10.1038/s41587-022-01652-0
https://doi.org/10.1038/s41587-022-01652-0
Autor:
Jens Füllgrabe, Walraj S Gosal, Páidí Creed, Sidong Liu, Casper K Lumby, David J Morley, Tobias W B Ost, Albert J Vilella, Shirong Yu, Helen Bignell, Philippa Burns, Tom Charlesworth, Beiyuan Fu, Howerd Fordham, Nick Harding, Olga Gandelman, Paula Golder, Christopher Hodson, Mengjie Li, Marjana Lila, Yang Liu, Joanne Mason, Jason Mellad, Jack Monahan, Oliver Nentwich, Alexandra Palmer, Michael Steward, Minna Taipale, Audrey Vandomme, Rita Santo San-Bento, Ankita Singhal, Julia Vivian, Natalia Wójtowicz, Nathan Williams, Nicolas J Walker, Nicola C H Wong, Gary Yalloway, Joanna D Holbrook, Shankar Balasubaramanian
DNA comprises molecular information stored via genetic bases (G, C, T, A) and also epigenetic bases, principally 5-methylcytosine (5mC) and 5-hydroxymethylcytosine (5hmC). Both genetic and epigenetic information are vital to our understanding of biol
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_________::ac2af7acf13559572475f1c21b6cbf27
https://doi.org/10.1101/2022.07.08.499285
https://doi.org/10.1101/2022.07.08.499285
Autor:
Simon Daff, Tobias W B Ost
Publikováno v:
Journal of Biological Chemistry. 280:965-973
Mammalian NO synthases catalyze the monooxygenation of L-arginine (L-Arg) to N-hydroxyarginine (NOHA) and the subsequent monooxygenation of this to NO and citrulline. Both steps proceed via formation of an oxyferrous heme complex and may ultimately l
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_________::02f54217b543c337818d8fa64397c009
https://doi.org/10.1074/jbc.m411191200
https://doi.org/10.1074/jbc.m411191200
Publikováno v:
Biochemical and Biophysical Research Communications. 325:1418-1423
Flavocytochrome P450 BM3 FMN domain is unique among the family of flavodoxins and homologues, in not forming a stable neutral blue FMN semiquinone radical. Anaerobic, one-electron reduction of the isolated domain over the pH 7–9.5 range showed that
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_dedup___::a1d37c5f303ac94c7c311b62dfa028c9
https://doi.org/10.1016/j.bbrc.2004.10.189
https://doi.org/10.1016/j.bbrc.2004.10.189
Publikováno v:
Biochemistry. 43:11035-11044
Electron transfer through neuronal nitric oxide synthase (nNOS) is regulated by the reversible binding of calmodulin (CaM) to the reductase domain of the enzyme, the conformation of which has been shown to be dependent on the presence of substrate, N
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_dedup___::a22d6082dd167df48e87ca412ed8c0e4
https://doi.org/10.1021/bi049312v
https://doi.org/10.1021/bi049312v
Autor:
Simon Daff, Caroline S Miles, Christopher G. Mowat, Jonathan P. Clark, Graeme A Reid, Stephen K Chapman, Malcolm D. Walkinshaw, Tobias W B Ost
Publikováno v:
Journal of the American Chemical Society. 125:15010-15020
In flavocytochrome P450 BM3, there is a conserved phenylalanine residue at position 393 (Phe393), close to Cys400, the thiolate ligand to the heme. Substitution of Phe393 by Ala, His, Tyr, and Trp has allowed us to modulate the reduction potential of
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_dedup___::04bbc72f4d5065ab9fea75cd9a3c2ff5
https://doi.org/10.1021/ja035731o
https://doi.org/10.1021/ja035731o
Publikováno v:
The Biochemist. 25:20-23
More than one-third of all enzymes catalyse the oxidation or reduction of a substrate and are therefore classed as oxidoreductases or redox enzymes. The, often complex, redox chemistry involved here is made possible by surprisingly few redox-active c
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_________::f6beac560146ce0570e8cdd5523d9921
https://doi.org/10.1042/bio02504020
https://doi.org/10.1042/bio02504020
Autor:
Christopher C. Moser, Tobias W B Ost, Simon Daff, Dominikus A. Lysek, Caroline S Miles, Stephen K Chapman, Kirsty J. McLean, P. Leslie Dutton, David Leys, Ker R. Marshall, Andrew W. Munro, Christopher C. Page
Publikováno v:
Trends in Biochemical Sciences. 27:250-257
Flavocytochrome P450 BM3 is a bacterial P450 system in which a fatty acid hydroxylase P450 is fused to a mammalian-like diflavin NADPH-P450 reductase in a single polypeptide. The enzyme is soluble (unlike mammalian P450 redox systems) and its fusion
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_dedup___::ea062504ed5bcd0949330bc3dc0f6f9b
https://doi.org/10.1016/s0968-0004(02)02086-8
https://doi.org/10.1016/s0968-0004(02)02086-8
Autor:
Myles A. Cheesman, Christopher G. Mowat, Paul Taylor, Malcolm D. Walkinshaw, Stephen K Chapman, Andrew W. Munro, Arthur K. Cho, Antonio Pesseguiero, Armand J. Fulco, Tobias W B Ost
Publikováno v:
Biochemistry. 40:13430-13438
In the preceding paper in this issue [Ost, T. W. B., Miles, C. S., Munro, A. W., Murdoch, J., Reid, G. A., and Chapman, S. K. (2001) Biochemistry 40, 13421-13429], we have established that the primary role of the phylogenetically conserved phenylalan
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_dedup___::6f4d83cc8b89449074a685a9b3e21788
https://doi.org/10.1021/bi010717e
https://doi.org/10.1021/bi010717e