Signal transduction in light-oxygen-voltage receptors lacking the active-site glutamine

Autor: Julia Dietler, Renate Gelfert, Jennifer Kaiser, Veniamin Borin, Christian Renzl, Sebastian Pilsl, Américo Tavares Ranzani, Andrés García de Fuentes, Tobias Gleichmann, Ralph P. Diensthuber, Michael Weyand, Günter Mayer, Igor Schapiro, Andreas Möglich

Publikováno v: Nature Communications, Vol 13, Iss 1, Pp 1-16 (2022)

Light-oxygen-voltage (LOV) photoreceptors perceive blue light to elicit spatio-temporally defined cellular responses, and their signalling process has been extensively characterized. Here the authors report that the light signal is still transduced i

Externí odkaz: https://doaj.org/article/f256fda69ce44cd4a46cdde307162433

Open microfluidic gel electrophoresis: Rapid and low cost separation and analysis of DNA at the nanoliter scale

Autor: Roland Zengerle, Peter Koltay, Laurent Tanguy, L. Riegger, Ludwig Gutzweiler, Tobias Gleichmann

Publikováno v: ELECTROPHORESIS. 38:1764-1770

Gel electrophoresis is one of the most applied and standardized tools for separation and analysis of macromolecules and their fragments in academic research and in industry. In this work we present a novel approach for conducting on-demand electropho

Externí odkaz: https://explore.openaire.eu/search/publication?articleId=doi_________::1a7b2ef95e309f8c2a57ed3ff316db4a
https://doi.org/10.1002/elps.201700001

Charting the Signal Trajectory in a Light-Oxygen-Voltage Photoreceptor by Random Mutagenesis and Covariance Analysis

Autor: Andreas Möglich, Tobias Gleichmann, Ralph P. Diensthuber

Publikováno v: Journal of Biological Chemistry. 288:29345-29355

Modular signal receptors empower organisms to process environmental stimuli into adequate physiological responses. At the molecular level, a sensor module receives signals and processes the inherent information into changes of biological activity of

Externí odkaz: https://explore.openaire.eu/search/publication?articleId=doi_dedup___::97b771d2675c0a5139790cadd8687bc3
https://doi.org/10.1074/jbc.m113.506139

Femto- to Microsecond Photodynamics of an Unusual Bacteriophytochrome

Autor: Janneke Ravensbergen, Kevin D. Gallagher, Emina A. Stojković, Tobias Gleichmann, Tilo Mathes, Svetlana E. Kovaleva, Nicole C. Woitowich, John T. M. Kennis, Miroslav Kloz, Rachael St. Peter

Publikováno v: Journal of Physical Chemistry Letters, 6(2), 239-243. American Chemical Society
Mathes, T, Ravensbergen, J, Kloz, M, Gleichmann, T, Gallagher, K D, Woitowich, N C, St. Peter, R, Kovaleva, S E, Stojković, E A & Kennis, J T M 2015, ' Femto-to Microsecond Photodynamics of an Unusual Bacteriophytochrome ', Journal of Physical Chemistry Letters, vol. 6, no. 2, pp. 239-243 . https://doi.org/10.1021/jz502408n

A bacteriophytochrome from Stigmatella aurantiaca is an unusual member of the bacteriophytochrome family that is devoid of hydrogen bonding to the carbonyl group of ring D of the biliverdin (BV) chromophore. The photodynamics of BV in SaBphP1 wild ty

Externí odkaz: https://explore.openaire.eu/search/publication?articleId=doi_dedup___::dbcdcc924a85311af00ce8bb57265fa4
https://research.vu.nl/en/publications/3943c45f-1e39-435b-b48a-eb7c3ff10da2

Biophysical, mutational, and functional investigation of the chromophore-binding pocket of light-oxygen-voltage photoreceptors

Autor: Christopher Engelhard, Robert Bittl, Ralph P. Diensthuber, Tobias Gleichmann, Nora Lemke, Robert Ohlendorf, Andreas Möglich

Publikováno v: ACS Synthetic Biology

As light-regulated actuators, sensory photoreceptors underpin optogenetics and numerous applications in synthetic biology. Protein engineering has been applied to fine-tune the properties of photoreceptors and to generate novel actuators. For the blu

Externí odkaz: https://explore.openaire.eu/search/publication?articleId=doi_dedup___::f448dea0710e413955dd86ba183597e3
https://hdl.handle.net/21.11116/0000-000C-BC15-9