Zobrazeno 1 - 10
of 47
pro vyhledávání: '"Tito Ureta"'
Autor:
TITO URETA
Publikováno v:
Biological Research, Vol 34, Iss 2, Pp 14-15 (2001)
Externí odkaz:
https://doaj.org/article/2e0b7220395740398e269dad750493ed
Publikováno v:
FEBS Letters. 581:663-666
Besides the classic direct route, frog oocytes incorporate glucosyl units into glycogen by the so-called indirect pathway. The operation of both pathways depends on glucose availability. Below 0.5 mM glucose (calculated intracellular concentration),
Publikováno v:
Archives of Biochemistry and Biophysics. 413:9-16
Frog oocyte glycogen synthase properties differ significantly under in vitro or in vivo conditions. The K(mapp) for UDP-glucose in vivo was 1.4mM (in the presence or absence of glucose-6-P). The in vitro value was 6mM and was reduced by glucose-6-P t
Publikováno v:
IUBMB Life (International Union of Biochemistry and Molecular Biology: Life). 51:5-10
This review is intended to illustrate how live frog oocytes may be advantageously used to address the study of some problems of in vivo glucose metabolism. Glucose microinjected into the cells is preferentially committed to glycogen synthesis. We pre
Publikováno v:
FEBS Letters. 475:145-149
Hexokinase and glucose-6-phosphate dehydrogenase activities were increased in Xenopus laevis oocytes by microinjection of commercial pure enzymes. The effect of increased fractional activities on glycogen synthesis or on the production of 14CO(2) (th
Publikováno v:
FEBS Letters. 446:149-152
Evolution of CO2 from labelled glucose microinjected into frog oocytes in vivo may be ascribed to the pentose-P pathway, as measured by radioactive CO2 production from [1-14C] and [6-14C]glucose. Coinjection of NADP+ and [14C]glucose significantly st
Publikováno v:
Biochimica et Biophysica Acta (BBA) - Molecular Cell Research. 1401:242-264
Autor:
Tito Ureta
Publikováno v:
Journal of Theoretical Biology. 152:81-84
Publikováno v:
Electrophoresis. 28(16)
Glycogen synthase catalyzes the incorporation of UDP-glucose into glycogen. The activity of the enzyme is usually measured either by a spectrophotometric method or by a radioassay. The first one is not suitable because of the difficulties regarding t
Publikováno v:
Archives of biochemistry and biophysics. 348(1)
Glycogen synthesis following glucose microinjection in frog oocytes proceeds preferentially by an indirect pathway involving gluconeogenesis from triose compounds. Because of the known regulatory role of fructose-2,6-bisP on glucose utilization in mo