Zobrazeno 1 - 9
of 9
pro vyhledávání: '"Tiny A. M. van der Velden-de Groot"'
Autor:
Tiny A. M. van der Velden-de Groot, E. Coen Beuvery, Jan W. Oberink, Freek Brunink, José M. Coco-Martin
Publikováno v:
Hybridoma. 11:653-665
In this study several analytical techniques were applied to obtain information about the stability of expression, the yield, and the integrity of a monoclonal antibody (Mab) produced by hybridoma cell line RIV6 in a homogeneous continuous perfusion c
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_dedup___::b925f0126509347adac48a2dd9b0ec4d
https://doi.org/10.1089/hyb.1992.11.653
https://doi.org/10.1089/hyb.1992.11.653
Autor:
E. Coen Beuvery, Daan J.A. Crommelin, Tiny A. M. van der Velden-de Groot, Anna-Myra V. Hoven, Wim Jiskoot, Jacqueline W.T.M. Klein Gebbinck, Jos J.C.C. Van Hertrooij
Publikováno v:
Journal of Immunological Methods. 138:273-283
Three mouse monoclonal antibodies (Mab), RIV6, MN12, and WT31, were purified from cell culture supernatants containing foetal bovine serum (FBS) by two-step purification protocols, involving protein A affinity and ion exchange chromatography. Provide
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_dedup___::267ffb9e25a40e2061124a76aea7d128
https://doi.org/10.1016/0022-1759(91)90176-g
https://doi.org/10.1016/0022-1759(91)90176-g
Publikováno v:
Analytica Chimica Acta. 249:257-262
Several analytical techniques were applied to obtain information about the stability of expression, yield and integrity of monoclonal antibodies (Mabs) produced by hybridoma cells in homogeneous continuous perfusion culture systems. An isotype-specif
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_________::7bab002a89c46312cdbc1d6ea701e4b6
https://doi.org/10.1016/0003-2670(91)87031-2
https://doi.org/10.1016/0003-2670(91)87031-2
Publikováno v:
Journal of Immunoassay. 11:31-47
This study describes the characterization of antibody labelled colloidal gold particles and their applicability in a sol particle immuno assay (SPIA) to quantify murine monoclonal antibodies of all IgG isotypes. Two physical methods (transmission ele
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_dedup___::461b78d6aeb4b2a9964b985892f6b95a
https://doi.org/10.1080/01971529008053256
https://doi.org/10.1080/01971529008053256
Publikováno v:
Cytotechnology. 13(3)
The stability of the hybridoma cell line MN12 in a long-term homogeneous continuous culture was studied using a panel of analytical methods. These include two flow cytometry methods, for the determination of relative cytoplasmic and membrane IgG cont
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_dedup___::e0a7149ba09ce9d5a9d2b7d3ffb56201
https://pubmed.ncbi.nlm.nih.gov/7764662
https://pubmed.ncbi.nlm.nih.gov/7764662
Publikováno v:
Journal of immunological methods. 155(2)
Lectins have been used for the determination of the oligosaccharide structures expressed by two monoclonal IgG antibodies, MN12 and RIV6. Dot blot experiments revealed the presence of terminal Fuc alpha (1--6)GlcNAc, Gal beta (1--3)GalNAc, Gal beta (
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_dedup___::394dbfb5e726bd659d4d8d1efe2f88d6
https://pubmed.ncbi.nlm.nih.gov/1431152
https://pubmed.ncbi.nlm.nih.gov/1431152
Publikováno v:
Cytotechnology. 8(1)
Flow cytometric (FC) analysis was applied to determine changes at cellular level during the cultivation of hybridoma cell line MN12 in a suspension batch culture. The relative cell size, cytoplasmic and membrane IgG content and the viability were mon
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_dedup___::cd534cafacb3dcb8e43efa5a91f6a99c
https://pubmed.ncbi.nlm.nih.gov/1368406
https://pubmed.ncbi.nlm.nih.gov/1368406
Publikováno v:
Cytotechnology. 8(1)
Several methods were applied to determine the viability of hybridoma cells in suspension. These methods include dye inclusion and exclusion assays such as the classical trypan blue exclusion assay, the propidium iodide (PI) exclusion assay and the fl
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_dedup___::281219e9072f5e5425e8734e3e340825
https://pubmed.ncbi.nlm.nih.gov/1368405
https://pubmed.ncbi.nlm.nih.gov/1368405
Autor:
Wim Jiskoot, Jacqueline W.T.M. Klein Gebbinck, Jos J.C.C. Van Hertrooij, Tiny A. M. van der Velden-de Groot, Daan J.A. Crommelin, E. Coen Beuvery
Publikováno v:
Journal of Immunological Methods. 124:143-156
The murine hybridoma cell line WT31, which produces a monoclonal antibody (Mab) of the IgG1 isotype with specificity for the human T cell receptor, was grown in batch-suspension cultures in the presence of foetal bovine serum (FBS). To acquire a clin
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_________::bd06d363f619e82343318b67c315ddba
https://doi.org/10.1016/0022-1759(89)90196-8
https://doi.org/10.1016/0022-1759(89)90196-8