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pro vyhledávání: '"Tineke Laura Lenstra"'
Autor:
Ineke Brouwer, Heta Piyush Patel, Joseph Victor Willem Meeussen, Wim Pomp, Tineke Laura Lenstra
Publikováno v:
STAR Protocols, Vol 1, Iss 3, Pp 100142- (2020)
Summary: This protocol describes how to image fluorescently tagged proteins, RNA, or DNA inside living Saccharomyces cerevisiae cells at the single-molecule level. Imaging inside living cells, as opposed to fixed materials, gives access to real-time
Externí odkaz:
https://doaj.org/article/978925e111a44a238ec5f246a4609285
Publikováno v:
Nature structural & molecular biology, 30(5), 692-702. Nature Publishing Group
SummaryTranscriptional bursting has been linked to the stochastic positioning of nucleosomes. However, how bursting is regulated by remodeling of promoter nucleosomes is unknown. Here, we use single-molecule live-cell imaging of GAL10 transcription i