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Autor:
Tineka Quinton-Jager, Geoffrey G. Wilson, Jack S. Benner, Rebecca Kucera, Laurie S. Moran, Ellen Guthrie, Barton E. Slatko, Joan E. Brooks
Publikováno v:
Gene. 180(1-2)
SphI, a type II restriction-modification (R-M) system from the bacterium Streptomyces phaeochromogenes, recognizes the sequence 5'-GCATGC. The SphI methyltransferase (MTase)-encoding gene, sphIM, was cloned into Escherichia coli using MTase selection