Zobrazeno 1 - 10
of 12
pro vyhledávání: '"Tina R. Lynch"'
Autor:
Scott Takeo Aoki, Tina R. Lynch, Sarah L. Crittenden, Craig A. Bingman, Marvin Wickens, Judith Kimble
Publikováno v:
Nature Communications, Vol 12, Iss 1, Pp 1-14 (2021)
Nematode P granules are cytoplasmic RNA–protein biomolecule condensates central to germ cell development. Here the authors show that dimerization of the PGL-1 scaffolding protein is crucial to granule formation and mRNA repression, and that the WAG
Externí odkaz:
https://doaj.org/article/1fc26fa5125a4471a584495d77e492d1
Publikováno v:
Open Biology, Vol 5, Iss 11 (2015)
The spindle assembly checkpoint is a surveillance mechanism that blocks anaphase onset until all chromosomes are properly attached to microtubules of the mitotic spindle. Checkpoint activity requires kinetochore localization of Mad1/Mad2 to inhibit a
Externí odkaz:
https://doaj.org/article/19f3d81e12dc41cc81a5d45f852b9a47
Publikováno v:
RNA
Robust methods are critical for testing the in vivo regulatory mechanism of RNA binding proteins. Here we report improvement of a protein–mRNA tethering assay to probe the function of an RNA binding protein in its natural context within the C. eleg
Publikováno v:
Development. 149
A long-standing biological question is how DNA cis-regulatory elements shape transcriptional patterns during metazoan development. The use of reporter constructs, cell culture and computational modeling has made enormous contributions to understandin
Publikováno v:
eLife, Vol 5 (2016)
C. elegans Notch signaling maintains a pool of germline stem cells within their single-celled mesenchymal niche. Here we investigate the Notch transcriptional response in germline stem cells using single-molecule fluorescence in situ hybridization co
Externí odkaz:
https://doaj.org/article/56590abae95c4d91bc2ce30764ada7f1
Autor:
Craig A. Bingman, Judith Kimble, Marvin Wickens, Scott Takeo Aoki, Sarah L. Crittenden, Tina R. Lynch
Publikováno v:
Nature Communications
Nature Communications, Vol 12, Iss 1, Pp 1-14 (2021)
Nature Communications, Vol 12, Iss 1, Pp 1-14 (2021)
Cytoplasmic RNA–protein (RNP) granules have diverse biophysical properties, from liquid to solid, and play enigmatic roles in RNA metabolism. Nematode P granules are paradigmatic liquid droplet granules and central to germ cell development. Here we
Robust methods are critical for testing thein vivoregulatory mechanism of RNA binding proteins. Here we report improvement of a protein-mRNA tethering assay to probe the function of an RNA binding protein in its natural context within theC. elegansad
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_________::447bd1ae880092f49f1d50ff98028f35
https://doi.org/10.1101/2021.01.24.428005
https://doi.org/10.1101/2021.01.24.428005
Autor:
Tina R. Lynch, Craig A. Bingman, Scott T. Aoki, Judith Kimble, Marvin Wickens, Sarah L. Crittenden
SummaryCytoplasmic RNA-protein (RNP) granules have diverse biophysical properties, from liquid to solid, and play enigmatic roles in RNA metabolism. Nematode P-granules are paradigmatic liquid droplet granules and central to germ cell development. He
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_dedup___::4b5edff5d0395358a6801d720bb9b40e
Autor:
Erika B. Sorensen, Sarah L. Crittenden, Judith Kimble, Hannah S. Seidel, ChangHwan Lee, Tina R. Lynch
Publikováno v:
Bio Protoc
Single-molecule RNA fluorescence in situ hybridization (smFISH) is a technique to visualize individual RNA molecules using multiple fluorescently-labeled oligonucleotide probes specific to the target RNA ( Raj et al., 2008 ; Lee et al., 2016a ). We a
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_dedup___::ce12c0c0bbf32b7d4896cb201c6e1c4b
https://europepmc.org/articles/PMC8410355/
https://europepmc.org/articles/PMC8410355/
Publikováno v:
eLife, Vol 5 (2016)
eLife
eLife
C. elegans Notch signaling maintains a pool of germline stem cells within their single-celled mesenchymal niche. Here we investigate the Notch transcriptional response in germline stem cells using single-molecule fluorescence in situ hybridization co