Zobrazeno 1 - 10
of 12
pro vyhledávání: '"Tina Etcheverry"'
Publikováno v:
Biotechnology Progress. 21:550-553
A recombinant Chinese hamster ovary (CHO) cell line was used to express a humanized antibody. Product quality analysis of this humanized antibody showed the presence of free thiol, due to unpaired cysteine residues in the Fab region. Decreased potenc
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_dedup___::df6279c28c933638615c74b102b4011a
https://doi.org/10.1021/bp0497029
https://doi.org/10.1021/bp0497029
Publikováno v:
Biotechnology and Bioengineering. 69:440-449
Several small-scale Chinese hamster ovary (CHO) suspension cultures were grown in perfusion mode using a new acoustic filtration system. The separation performance was evaluated at different cell concentrations and perfusion rates for two different C
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_dedup___::31c5d21603c25c272cd4cbdc0d7397dc
https://doi.org/10.1002/1097-0290(20000820)69:4<440::aid-bit10>3.0.co
https://doi.org/10.1002/1097-0290(20000820)69:4<440::aid-bit10>3.0.co
Autor:
Tina Etcheverry, Jennie P. Mather, Kenneth D. Bauer, Christopher J. Donahue, Alison Moore, Jennifer Mercer, Thomas Ryll, George Dutina
Publikováno v:
Cytotechnology. 23:47-54
Temperature reduction in CHO cell batch culture may be beneficial in the production of recombinant protein and in maintenance of viability. The effects on cell cycle, apoptosis and nucleotide pools were studied in cultures initiated at 37°C and temp
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_________::c2c72a4f6506b4d71e2c2fbcc81044c0
https://doi.org/10.1023/a:1007919921991
https://doi.org/10.1023/a:1007919921991
Publikováno v:
Journal of Chromatography A. 753:73-80
Adsorption chromatography using underivatized porous glass can be an effective capture step for the purification of recombinant proteins. Classical desorption techniques using chaotropic agents or harsh chemical solvents often result in elution of in
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_dedup___::28439ad9b377cbe025f54ae8c64d9ee7
https://doi.org/10.1016/s0021-9673(96)00549-3
https://doi.org/10.1016/s0021-9673(96)00549-3
Publikováno v:
Animal Cell Technology: From Target to Market ISBN: 9789401038973
A stepwise factorial design and analysis approach was pursued for the development of a recombinant antibody process in CHO cells. The influence of eleven factors on cell growth and metabolism, viability, productivity and product quality were measured
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_________::785f971fdaf951e82d9000682b1461ea
https://doi.org/10.1007/978-94-010-0369-8_66
https://doi.org/10.1007/978-94-010-0369-8_66
Autor:
Lydia Santell, Michael Santoris, Thomas G. Warner, George Dutina, Jane Gunson, Thomas Ryll, Angie Wang, Tina Etcheverry
Publikováno v:
Biochemical and biophysical research communications. 258(1)
The incorporation of sialic acid into therapeutic recombinant glycoprotein expressed in Chinese hamster ovary (CHO) cells during growth in large bioreactors (10 l) has been monitored under high productivity conditions induced by the presence of sodiu
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_dedup___::c89fd95af4bf8d96ebaefc44c5e55d2f
https://pubmed.ncbi.nlm.nih.gov/10222248
https://pubmed.ncbi.nlm.nih.gov/10222248
Publikováno v:
Animal Cell Technology ISBN: 9789401062732
N-linked Oligosaccharides on a recombinant protein play a critical role in the metabolic behavior of the molecule. The extent of glycosylation on the protein can determine clearance rates and the ultimate pharmacokinctic profile. The amount of termin
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_________::9c8e119fc830ef71ce487a936294d918
https://doi.org/10.1007/978-94-011-5404-8_101
https://doi.org/10.1007/978-94-011-5404-8_101
Publikováno v:
The Journal of Cell Biology
Yeast cell surface growth is accomplished by constitutive secretion and plasma membrane assembly, culminating in the fusion of vesicles with the bud membrane. Coordination of secretion and membrane assembly has been investigated by examining the biog
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_dedup___::433f33b987d1bdbcaa140214031c3ba1
https://doi.org/10.1083/jcb.106.3.641
https://doi.org/10.1083/jcb.106.3.641
Publikováno v:
Analytical Biochemistry. 166:328-334
Purification of secretory vesicles from Saccharomyces cerevisiae has been hindered because these organelles normally represent a small proportion of cellular membranes. In the yeast secretory mutant sec1, secretory vesicles accumulate intracellularly
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_dedup___::3b8e679116cb0665dcbb3b4ebc7f4305
https://doi.org/10.1016/0003-2697(87)90581-1
https://doi.org/10.1016/0003-2697(87)90581-1