Zobrazeno 1 - 10 of 10 pro vyhledávání: '"Tina, Xiong"'
2
Akademický článek
Targeted DNA methylation in human cells using engineered dCas9-methyltransferases
Autor: Tina Xiong, Glenna E. Meister, Rachael E. Workman, Nathaniel C. Kato, Michael J. Spellberg, Fulya Turker, Winston Timp, Marc Ostermeier, Carl D. Novina
Publikováno v: Scientific Reports, Vol 7, Iss 1, Pp 1-14 (2017)
Abstract Mammalian genomes exhibit complex patterns of gene expression regulated, in part, by DNA methylation. The advent of engineered DNA methyltransferases (MTases) to target DNA methylation to specific sites in the genome will accelerate many are
Externí odkaz: https://doaj.org/article/0f956c0b5d804d3494852eec91096851
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3
Akademický článek
Protein engineering strategies for improving the selective methylation of target CpG sites by a dCas9-directed cytosine methyltransferase in bacteria.
Autor: Tina Xiong, Dahlia Rohm, Rachael E Workman, Lauren Roundtree, Carl D Novina, Winston Timp, Marc Ostermeier
Publikováno v: PLoS ONE, Vol 13, Iss 12, p e0209408 (2018)
Mammalian gene expression is a complex process regulated in part by CpG methylation. The ability to target methylation for de novo gene regulation could have therapeutic and research applications. We have previously developed a dCas9-MC/MN protein fo
Externí odkaz: https://doaj.org/article/65e27a1bee884baf872e33c2213dccdc
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5
Targeted DNA methylation in human cells using engineered dCas9-methyltransferases
Autor: Marc Ostermeier, Fulya Turker, Winston Timp, Michael J. Spellberg, Glenna E. Meister, Rachael E. Workman, Tina Xiong, Carl D. Novina, Nathaniel C. Kato
Publikováno v: Scientific Reports, Vol 7, Iss 1, Pp 1-14 (2017)
Scientific Reports
Mammalian genomes exhibit complex patterns of gene expression regulated, in part, by DNA methylation. The advent of engineered DNA methyltransferases (MTases) to target DNA methylation to specific sites in the genome will accelerate many areas of bio
Externí odkaz: https://explore.openaire.eu/search/publication?articleId=doi_dedup___::a28a9a477a2aba74b7e78dba57970102
https://doaj.org/article/0f956c0b5d804d3494852eec91096851
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6
Protein-surfactant-polysaccharide nanoparticles increase the catalytic activity of an engineered β-lactamase maltose-activated switch enzyme
Autor: Francisco M. Goycoolea, Bruno M. Moerschbacher, Tina Xiong, Marc Ostermeier, J. P. Fuenzalida
We present polysaccharide-based nanoparticles able to associate and increase the catalytic activity of the maltose-binding MBP317-347 switch enzyme. Fluorescence quenching and molecular docking studies along with the partial resistance to increasing
Externí odkaz: https://explore.openaire.eu/search/publication?articleId=doi_dedup___::142def98895c4c420ae0cdfa504b630c
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7
The interplay between effector binding and allostery in an engineered protein switch
Autor: Jay H. Choi, Marc Ostermeier, Tina Xiong
Publikováno v: Protein Science. 25:1605-1616
The protein design rules for engineering allosteric regulation are not well understood. A fundamental understanding of the determinants of ligand binding in an allosteric context could facilitate the design and construction of versatile protein switc
Externí odkaz: https://explore.openaire.eu/search/publication?articleId=doi_dedup___::dd560a21921e9a156afe62a5ec3bc990
https://doi.org/10.1002/pro.2962
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8
Molecular Determinants for Protein Stabilization by Insertional Fusion to a Thermophilic Host Protein
Autor: Brennal Pierre, Edwin Aoraha, Jin Ryoun Kim, Asher Williams, Edward Chau, Jeffrey J. Gray, Tina Xiong, Jason W. Labonte, Kazi Yasin Helal, Vandan Shah
Publikováno v: ChemBioChem. 16:2392-2402
A universal method that improves protein stability and evolution has thus far eluded discovery. Recently, however, studies have shown that insertional fusion to a protein chaperone stabilized various target proteins with minimal negative effects. The
Externí odkaz: https://explore.openaire.eu/search/publication?articleId=doi_dedup___::10f8d3eec47c7f078e63d1c4299f6c2a
https://doi.org/10.1002/cbic.201500310
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9
Protein engineering strategies for improving the selective methylation of target CpG sites by a dCas9-directed cytosine methyltransferase in bacteria
Autor: Dahlia Rohm, Winston Timp, Tina Xiong, Rachael E. Workman, Marc Ostermeier, Lauren Roundtree, Carl D. Novina
Publikováno v: PLoS ONE, Vol 13, Iss 12, p e0209408 (2018)
PLoS ONE
Mammalian gene expression is a complex process regulated in part by CpG methylation. The ability to target methylation for de novo gene regulation could have therapeutic and research applications. We have previously developed a dCas9-MC/MN protein fo
Externí odkaz: https://explore.openaire.eu/search/publication?articleId=doi_dedup___::59ca8afff01f00d25652c38a5612040a
https://doi.org/10.1371/journal.pone.0209408
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10
Stability of a guest protein depends on stability of a host protein in insertional fusion
Autor: Leonie Hayles, Brennal Pierre, Visweswara Reddy Guntaka, Tina Xiong, Jin Ryoun Kim
Publikováno v: Biotechnology and bioengineering. 108(5)
Insertional fusion between host and guest protein domains has been employed to create multi-domain protein complexes displaying integrated and coupled functionalities. The effects of insertional fusion on the stability of a guest protein are however
Externí odkaz: https://explore.openaire.eu/search/publication?articleId=doi_dedup___::da963bbb0e161fc4ed0989e844efdbfc
https://pubmed.ncbi.nlm.nih.gov/21190177
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