Zobrazeno 1 - 5
of 5
pro vyhledávání: '"Timothy P. Newing"'
Publikováno v:
Engineering Microbiology, Vol 4, Iss 1, Pp 100120- (2024)
Recombineering is an essential tool for molecular biologists, allowing for the facile and efficient manipulation of bacterial genomes directly in cells without the need for costly and laborious in vitro manipulations involving restriction enzymes. Th
Externí odkaz:
https://doaj.org/article/26db5ad3b59847bd925a78c8f3e21d7a
Autor:
Timothy P. Newing, Jodi L. Brewster, Lucy J. Fitschen, James C. Bouwer, Nikolas P. Johnston, Haibo Yu, Gökhan Tolun
Publikováno v:
Nature Communications, Vol 13, Iss 1, Pp 1-14 (2022)
Redβ annealase catalyses single-strand annealing homologous DNA recombination. Here, the authors present a cryo-EM structure of a Redβ annealing intermediate bound to two complementary 27mer oligonucleotides.
Externí odkaz:
https://doaj.org/article/d8eae846edef420e98dca3a781c83034
Autor:
Timothy P. Newing, Aaron J. Oakley, Michael Miller, Catherine J. Dawson, Simon H. J. Brown, James C. Bouwer, Gökhan Tolun, Peter J. Lewis
Publikováno v:
Nature Communications, Vol 11, Iss 1, Pp 1-11 (2020)
Gram-positive bacteria contain a transcription factor HelD that is able to remove and recycle stalled transcription complexes. Here the authors provide mechanistic insights into this process by determining the cryo-EM structures of the Bacillus subti
Externí odkaz:
https://doaj.org/article/5ba51ba3f90b4dc994ae7757d1617c0b
Autor:
Timothy P, Newing, Jodi L, Brewster, Lucy J, Fitschen, James C, Bouwer, Nikolas P, Johnston, Haibo, Yu, Gökhan, Tolun
Publikováno v:
Nature communications. 13(1)
The Redβ protein of the bacteriophage λ red recombination system is a model annealase which catalyzes single-strand annealing homologous DNA recombination. Here we present the structure of a helical oligomeric annealing intermediate of Redβ, consi
Autor:
Catherine J. Dawson, James C. Bouwer, Aaron J. Oakley, Timothy P. Newing, Michael Miller, Gökhan Tolun, Simon H. J. Brown, Peter J. Lewis
Publikováno v:
Nature Communications, Vol 11, Iss 1, Pp 1-11 (2020)
Nature Communications
Nature Communications
In bacteria, transcription complexes stalled on DNA represent a major source of roadblocks for the DNA replication machinery that must be removed in order to prevent damaging collisions. Gram-positive bacteria contain a transcription factor HelD that