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pro vyhledávání: '"Timothy L. Brooks"'
Autor:
Melvin H. Green, Timothy L. Brooks
Publikováno v:
Nucleic Acids Research. 4:4279-4290
A small fraction of the SV40 chromatin isolated from infected monkey cell cultures by the Triton method contains active RNA polymerase which had initiated transcription in vivo. This viral transcription complex (VTC) was utilized to answer the questi
Autor:
Melvin H. Green, Timothy L. Brooks
Publikováno v:
Nucleic Acids Research. 4:4261-4278
SV40 chromatin obtained from infected monkey cells was used to study the effect of the viral chromatin proteins on endogenous RNA polymerase II. Ammonium sulfate activated the rate of transcription by endogenous RNA polymerase in two ways: 1) by dire
Autor:
Melvin H. Green, Timothy L. Brooks
Publikováno v:
Virology. 72(1)
Triton extraction of SV40 infected monkey cells separates the viral transcription complex (VTC) into two fractions. Both forms of VTC are comprised of viral DNA associated with RNA polymerase which had initiated RNA synthesis prior to extraction. App
Autor:
Timothy L. Brooks, Melvin H. Green
Publikováno v:
Nucleic acids research. 7(6)
Histone-deficient SV40 chromatin, selectively radiolabeled in the DNA following the addition of cycloheximide to infected monkey cells, was compared with the normal 55S viral chromatin for its ability to serve as a template for a subsequent round of