Zobrazeno 1 - 10
of 216
pro vyhledávání: '"Timothy K. Lu"'
Publikováno v:
Nature Communications, Vol 15, Iss 1, Pp 1-17 (2024)
Abstract Sophisticated gene circuits built by synthetic biology can enable bacteria to sense their environment and respond predictably. Engineered biosensing bacteria outfitted with such circuits can potentially probe the human gut microbiome to prev
Externí odkaz:
https://doaj.org/article/16a155c2953f4ff78cb197b9d63816ff
Autor:
Nicholas W. Frankel, Han Deng, Gozde Yucel, Marcus Gainer, Nelia Leemans, Alice Lam, Yongshuai Li, Michelle Hung, Derrick Lee, Chen-Ting Lee, Andrew Banicki, Mengxi Tian, Niran Almudhfar, Lawrence Naitmazi, Assen Roguev, Seunghee Lee, Wilson Wong, Russell Gordley, Timothy K. Lu, Brian S. Garrison
Publikováno v:
Cell Reports, Vol 43, Iss 5, Pp 114145- (2024)
Summary: Acute myeloid leukemia (AML) is an aggressive disease with a poor prognosis (5-year survival rate of 30.5% in the United States). Designing cell therapies to target AML is challenging because no single tumor-associated antigen (TAA) is highl
Externí odkaz:
https://doaj.org/article/e70e82d168324cb18d830c62e4a537dd
Autor:
William C. W. Chen, Leonid Gaidukov, Yong Lai, Ming-Ru Wu, Jicong Cao, Michael J. Gutbrod, Gigi C. G. Choi, Rachel P. Utomo, Ying-Chou Chen, Liliana Wroblewska, Manolis Kellis, Lin Zhang, Ron Weiss, Timothy K. Lu
Publikováno v:
Nature Communications, Vol 13, Iss 1, Pp 1-16 (2022)
Precise and scalable regulation of gene expression in mammalian cells is challenging. Here, the authors created a highly tunable CRISPR-based synthetic transcription system for programmable control of mammalian gene expression and cellular activity.
Externí odkaz:
https://doaj.org/article/310dc9b5a9a34df28dfb75c848485687
Publikováno v:
Materials Today Bio, Vol 18, Iss , Pp 100504- (2023)
Natural materials such as bone, wood, and bamboo can inspire the fabrication of stiff, lightweight structural materials. Biofilms are one of the most dominant forms of life in nature. However, little is known about their physical properties as a stru
Externí odkaz:
https://doaj.org/article/cf608df5e34a4ad89a683fedee9ab9ee
Autor:
Jicong Cao, Eva Maria Novoa, Zhizhuo Zhang, William C. W. Chen, Dianbo Liu, Gigi C. G. Choi, Alan S. L. Wong, Claudia Wehrspaun, Manolis Kellis, Timothy K. Lu
Publikováno v:
Nature Communications, Vol 12, Iss 1, Pp 1-10 (2021)
The engineering of 5′ UTRs that modulate protein expression remains a great challenge. Here we leverage synthetic biology and computational design to develop a high-throughput strategy to design, screen, and optimize 5′ UTRs that enhance protein
Externí odkaz:
https://doaj.org/article/7874f9ea0bf346329b2ba9f6b304ff50
Publikováno v:
Nature Communications, Vol 12, Iss 1, Pp 1-9 (2021)
There are few robust circuit architectures for sequential gene perturbations. Here, the authors use a modular recombinase-based design that sequentially edits loci, synchronizes cells, and deletes itself.
Externí odkaz:
https://doaj.org/article/0c21854435d549eeaf4ce8f46f2e69c0
Publikováno v:
BMC Biology, Vol 19, Iss 1, Pp 1-19 (2021)
Abstract Background Iron is essential for bacterial survival. Bacterial siderophores are small molecules with unmatched capacity to scavenge iron from proteins and the extracellular milieu, where it mostly occurs as insoluble Fe3+. Siderophores chela
Externí odkaz:
https://doaj.org/article/a518f0dd6ede4e6ea1feba1061440401
Autor:
Diana P. Pires, Rodrigo Monteiro, Dalila Mil-Homens, Arsénio Fialho, Timothy K. Lu, Joana Azeredo
Publikováno v:
Scientific Reports, Vol 11, Iss 1, Pp 1-10 (2021)
Abstract In the era where antibiotic resistance is considered one of the major worldwide concerns, bacteriophages have emerged as a promising therapeutic approach to deal with this problem. Genetically engineered bacteriophages can enable enhanced an
Externí odkaz:
https://doaj.org/article/ae5ac1d34e5c4cd7b8a8b178354b4d5a
Autor:
Jacqueline A. Valeri, Katherine M. Collins, Pradeep Ramesh, Miguel A. Alcantar, Bianca A. Lepe, Timothy K. Lu, Diogo M. Camacho
Publikováno v:
Nature Communications, Vol 11, Iss 1, Pp 1-14 (2020)
The design of synthetic biology circuits remains challenging due to poorly understood design rules. Here the authors introduce STORM and NuSpeak, two deep-learning architectures to characterize and optimize toehold switches.
Externí odkaz:
https://doaj.org/article/d92e1c3c09eb4750aa706310533722cf
Publikováno v:
mBio, Vol 12, Iss 6 (2021)
ABSTRACT Shigellosis causes most diarrheal deaths worldwide, particularly affecting children. Shigella invades and replicates in the epithelium of the large intestine, eliciting inflammation and tissue destruction. To understand how Shigella rewires
Externí odkaz:
https://doaj.org/article/15e9da1bb1a442e4a77457edf8c65820