Zobrazeno 1 - 10
of 88
pro vyhledávání: '"Timothy J. Stasevich"'
Autor:
Malgorzata J. Latallo, Shaopeng Wang, Daoyuan Dong, Blake Nelson, Nathan M. Livingston, Rong Wu, Ning Zhao, Timothy J. Stasevich, Michael C. Bassik, Shuying Sun, Bin Wu
Publikováno v:
Nature Communications, Vol 14, Iss 1, Pp 1-18 (2023)
Abstract C9ORF72 hexanucleotide repeat expansion is the most common genetic cause of both amyotrophic lateral sclerosis (ALS) and frontotemporal dementia (FTD). One pathogenic mechanism is the accumulation of toxic dipeptide repeat (DPR) proteins lik
Externí odkaz:
https://doaj.org/article/ab9f2ebcd16e4163807ff2dcc11f4974
Autor:
Chloe L. Barrington, Gabriel Galindo, Amanda L. Koch, Emma R. Horton, Evan J. Morrison, Samantha Tisa, Timothy J. Stasevich, Olivia S. Rissland
Publikováno v:
Cell Reports, Vol 42, Iss 12, Pp 113413- (2023)
Summary: Nonoptimal synonymous codons repress gene expression, but the underlying mechanisms are poorly understood. We and others have previously shown that nonoptimal codons slow translation elongation speeds and thereby trigger messenger RNA (mRNA)
Externí odkaz:
https://doaj.org/article/b3483aeae2a8422cb39ab1f4d60698c3
Autor:
Charlotte A. Cialek, Gabriel Galindo, Tatsuya Morisaki, Ning Zhao, Taiowa A. Montgomery, Timothy J. Stasevich
Publikováno v:
Nature Communications, Vol 13, Iss 1, Pp 1-14 (2022)
Guided by miRNA, Argonaute proteins silence mRNA in multiple ways that are not well understood. Here, the authors develop live-cell biosensors to image the impact tethered regulatory factors, such as Argonaute, have on single-mRNA translation dynamic
Externí odkaz:
https://doaj.org/article/f478678d329f413388568ca1d7cd8e98
Autor:
William S. Raymond, Sadaf Ghaffari, Luis U. Aguilera, Eric Ron, Tatsuya Morisaki, Zachary R. Fox, Michael P. May, Timothy J. Stasevich, Brian Munsky
Publikováno v:
Frontiers in Cell and Developmental Biology, Vol 11 (2023)
mRNA translation is the ubiquitous cellular process of reading messenger-RNA strands into functional proteins. Over the past decade, large strides in microscopy techniques have allowed observation of mRNA translation at a single-molecule resolution f
Externí odkaz:
https://doaj.org/article/d8c230a85e8541d088a66298ef414a15
Autor:
Linda S. Forero-Quintero, William Raymond, Tetsuya Handa, Matthew N. Saxton, Tatsuya Morisaki, Hiroshi Kimura, Edouard Bertrand, Brian Munsky, Timothy J. Stasevich
Publikováno v:
Nature Communications, Vol 12, Iss 1, Pp 1-16 (2021)
During transcription, RNA polymerase II (RNAP2) is recruited to promoters and phosphorylated stepwise; so far, these steps have not been visualized in a single-copy gene in live cells. Here the authors use single-molecule microscopy to visualize endo
Externí odkaz:
https://doaj.org/article/9ac9a177c745472c9f09664f8916d4d9
Autor:
Ning Zhao, Kouta Kamijo, Philip D. Fox, Haruka Oda, Tatsuya Morisaki, Yuko Sato, Hiroshi Kimura, Timothy J. Stasevich
Publikováno v:
Nature Communications, Vol 10, Iss 1, Pp 1-16 (2019)
Expression of genetically encoded antibodies for cell labelling is often limited by folding issues. Here, the authors engineer an anti-HA scFv antibody that works in the cellular environment and use it to track mRNA translation dynamics in living cel
Externí odkaz:
https://doaj.org/article/609e6cb7c33d4624985b65a876ea31c0
Autor:
Rajarshi P. Ghosh, Quanming Shi, Linfeng Yang, Michael P. Reddick, Tatiana Nikitina, Victor B. Zhurkin, Polly Fordyce, Timothy J. Stasevich, Howard Y. Chang, William J. Greenleaf, Jan T. Liphardt
Publikováno v:
Nature Communications, Vol 10, Iss 1, Pp 1-16 (2019)
Satb1 is a master regulator of multiple cellular processes. Here the authors find that Satb1 preferentially targets nucleosome dense regions and combinatorially uses multiple selection criteria including DNA torsion, flanking DNA shape, motif density
Externí odkaz:
https://doaj.org/article/c785b7867538444e850f8a448f988344
Autor:
Kari H. Ecklund, Tatsuya Morisaki, Lindsay G. Lammers, Matthew G. Marzo, Timothy J. Stasevich, Steven M. Markus
Publikováno v:
Nature Communications, Vol 8, Iss 1, Pp 1-16 (2017)
Dynein is a microtubule motor the motility of which is affected by the microtubule-associated protein She1. Here, the authors show that She1 alters dynein stepping behavior and increases its microtubule affinity through simultaneous interactions with
Externí odkaz:
https://doaj.org/article/913bdefabfaa49f98b3f7aa602394933
Autor:
Keith F DeLuca, Jeanne E Mick, Amy H Ide, Wanessa C Lima, Lori Sherman, Kristin L Schaller, Steven M Anderson, Ning Zhao, Timothy J Stasevich, Dileep Varma, Jakob Nilsson, Jennifer G DeLuca
Publikováno v:
eLife, Vol 10 (2021)
Antibodies are indispensable tools used for a large number of applications in both foundational and translational bioscience research; however, there are drawbacks to using traditional antibodies generated in animals. These include a lack of standard
Externí odkaz:
https://doaj.org/article/702303f606c44e00b209f26179885455
Post-translational protein modifications play an important role in the regulation of gene dynamics. Certain modifications, such as histone acetylation and RNA polymerase II phosphorylation, are associated with transcriptionally active chromatin. Howe
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_________::de2cca89fd6ab02803fdfcba19843f36
https://doi.org/10.1101/2023.03.02.530854
https://doi.org/10.1101/2023.03.02.530854
