Zobrazeno 1 - 10
of 70
pro vyhledávání: '"Timothy J. Doran"'
Publikováno v:
Methods and Protocols, Vol 5, Iss 1, p 18 (2022)
Exogenous RNA polymerase III (pol III) promoters are commonly used to express short hairpin RNA (shRNA). Previous studies have indicated that expression of shRNAs using standard pol III promoters can cause toxicity in vivo due to saturation of the na
Externí odkaz:
https://doaj.org/article/a9442f14327143e8b6319f4e51126b88
Autor:
Caitlin A. Cooper, Timothy J. Doran, Arjun Challagulla, Mark L. V. Tizard, Kristie A. Jenkins
Publikováno v:
Journal of Animal Science and Biotechnology, Vol 9, Iss 1, Pp 1-7 (2018)
Abstract The tools available for genome engineering have significantly improved over the last 5 years, allowing scientist to make precise edits to the genome. Along with the development of these new genome editing tools has come advancements in techn
Externí odkaz:
https://doaj.org/article/8b785e1ccb254fa0b8b84aea97062f9c
Publikováno v:
Methods and Protocols, Vol 4, Iss 2, p 40 (2021)
Advances in the field of CRISPR/Cas systems are expanding our ability to modulate cellular genomes and transcriptomes precisely and efficiently. Here, we assessed the Cas13a-mediated targeted disruption of RNA in chicken fibroblast DF1 cells. First,
Externí odkaz:
https://doaj.org/article/790165d038ec4ba6ae0bbfe82dbc3a41
Autor:
Arjun Challagulla, Kristie A. Jenkins, Terri E. O’Neil, Shunning Shi, Kirsten R. Morris, Terry G. Wise, Prasad N. Paradkar, Mark L. Tizard, Timothy J. Doran, Karel A. Schat
Publikováno v:
Microorganisms, Vol 9, Iss 1, p 164 (2021)
Marek’s disease (MD), caused by MD herpesvirus (MDV), is an economically important disease in chickens. The efficacy of the existing vaccines against evolving virulent stains may become limited and necessitates the development of novel antiviral st
Externí odkaz:
https://doaj.org/article/c9ca6b57a53f40fe882f9d484bfc93f7
Autor:
Mark L Tizard, Terri E. O’Neil, Shunning Shi, Kirsten R. Morris, Kiran Nair, Timothy J. Doran, Kristie A. Jenkins, Terry G. Wise, Arjun Challagulla, David M. Cahill
Publikováno v:
Animal Biotechnology. 33:1235-1245
Efficient isolation of genetically modified cells that are phenotypically indistinguishable from the unmodified cells remains a major technical barrier for the broader utilization of CRISPR/Cas9. Here, we report a novel enrichment approach to select
Autor:
Mark Tizard, Andrew G. D. Bean, Terri E. O’Neil, Karel A. Schat, Kirsten R. Morris, Terry G. Wise, Kristie A. Jenkins, Arjun Challagulla, Timothy J. Doran
Publikováno v:
Animal Biotechnology. :1-10
Development of simple and readily adoptable methods to mediate germline engineering of the chicken genome will have many applications in research, agriculture and industrial biotechnology. We report germline targeting of the endogenous chicken Interf
Publikováno v:
PLoS ONE, Vol 8, Iss 6, p e68362 (2013)
Estrogens play a key role in sexual differentiation of both the gonads and external traits in birds. The production of estrogen occurs via a well-characterised steroidogenic pathway, which is a multi-step process involving several enzymes, including
Externí odkaz:
https://doaj.org/article/0f378e27f94d4fc2b0fde4c6d4f3d3b4
Autor:
Arjun, Challagulla, Kristie A, Jenkins, Terri E, O'Neil, Kirsten R, Morris, Terry G, Wise, Mark L, Tizard, Andrew G D, Bean, Karel A, Schat, Timothy J, Doran
Publikováno v:
Animal biotechnology.
Development of simple and readily adoptable methods to mediate germline engineering of the chicken genome will have many applications in research, agriculture and industrial biotechnology. We report germline targeting of the endogenous chicken Interf
Autor:
Jerome Gros, Caitlin Cooper, Marie Julie Dejardin, Christophe Marcelle, Timothy J. Doran, David Salgado, Nadège Véron, Olivier Serralbo
Publikováno v:
eLife
eLife, 2020, 9, ⟨10.7554/eLife.56312⟩
eLife, Vol 9 (2020)
eLife, eLife Sciences Publication, 2020, 9, ⟨10.7554/eLife.56312⟩
eLife, 2020, 9, ⟨10.7554/eLife.56312⟩
eLife, Vol 9 (2020)
eLife, eLife Sciences Publication, 2020, 9, ⟨10.7554/eLife.56312⟩
International audience; Due to its amenability to manipulations, to live observation and its striking similarities to mammals, the chicken embryo has been one of the major animal models in biomedical research. Although it is technically possible to g
Autor:
Caitlin Cooper, Marie-Julie Dejardin, David Salgado, Christophe Marcelle, Jerome Gros, Olivier Serralbo, Timothy J. Doran, Nadège Véron
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_________::d8935f352943a0a07e8497aa52729369
https://doi.org/10.7554/elife.56312.sa2
https://doi.org/10.7554/elife.56312.sa2