Zobrazeno 1 - 10
of 28
pro vyhledávání: '"Timothy H. Bayburt"'
Autor:
Andrew J. Leitz, Timothy H. Bayburt, Alexander N. Barnakov, Barry A. Springer, Stephen G. Sligar
Publikováno v:
BioTechniques, Vol 40, Iss 5, Pp 601-612 (2006)
Integral membrane G protein-coupled receptors (GPCRs) compose the single most prolific class of drug targets, yet significant functional and structural questions remain unanswered for this superfamily. A primary reason for this gap in understanding a
Externí odkaz:
https://doaj.org/article/889f981fe4154c61b5c3ee3cd13590b2
Publikováno v:
BioTechniques, Vol 35, Iss 3, Pp 556-563 (2003)
One of the biggest challenges in the field of proteomics is obtaining functional membrane proteins solubilized and dispersed into a physiologically relevant environment that maintains the spectrum of in vivo activities. Here we describe a system comp
Externí odkaz:
https://doaj.org/article/35f13ce3cf8e4d528651f9a07c4070b4
Autor:
Takefumi Morizumi, Ana Karin Kusnetzow, Lydia N. Caro, Ned Van Eps, Oliver P. Ernst, Timothy H. Bayburt, Klaus Peter Hofmann, Wayne L. Hubbell, Stephen G. Sligar, Michal Szczepek
Publikováno v:
Proceedings of the National Academy of Sciences of the United States of America, vol 114, iss 16
Conformational equilibria of G-protein–coupled receptors (GPCRs) are intimately involved in intracellular signaling. Here conformational substates of the GPCR rhodopsin are investigated in micelles of dodecyl maltoside (DDM) and in phospholipid nan
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_dedup___::c25c920a317666de4cdfecbb0df4df56
https://escholarship.org/uc/item/3c01m5gt
https://escholarship.org/uc/item/3c01m5gt
Autor:
Brett R. Goldsmith, Joseph J. Mitala, Jesusa Josue, Ana Castro, Mitchell B. Lerner, Timothy H. Bayburt, Samuel M. Khamis, Ryan A. Jones, Joseph G. Brand, Stephen G. Sligar, Charles W. Luetje, Alan Gelperin, Paul A. Rhodes, Bohdana M. Discher, A. T. Charlie Johnson
Publikováno v:
ACS Nano. 5:5408-5416
We have designed and implemented a practical nanoelectronic interface to G-protein coupled receptors (GPCRs), a large family of membrane proteins whose roles in the detection of molecules outside eukaryotic cells make them important pharmaceutical ta
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_dedup___::1cb83e227cd0de37534f8cd1139d824e
https://doi.org/10.1021/nn200489j
https://doi.org/10.1021/nn200489j
Autor:
Yelena V. Grinkova, Daniel A. Heller, Aidas J. Mattis, Colin A. Wraight, Jong Hyun Choi, Timothy H. Bayburt, Adam S. Zeiger, Moon-Ho Ham, Esther S. Jeng, Alice C. Chang, Rachel A. Graff, Erik K. Hobbie, Michael S. Strano, Stephen G. Sligar, Ardemis A. Boghossian, Krystyn J. Van Vliet
Publikováno v:
Nature chemistry
Naturally occurring photosynthetic systems use elaborate pathways of self-repair to limit the impact of photo-damage. Here, we demonstrate a complex consisting of two recombinant proteins, phospholipids and a carbon nanotube that mimics this process.
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_dedup___::dfbe573daf07df1c3d1908644c7a3195
https://doi.org/10.1038/nchem.822
https://doi.org/10.1038/nchem.822
Autor:
Timothy H. Bayburt, Stephen G. Sligar
Publikováno v:
FEBS Letters. 584:1721-1727
Nanodiscs are soluble nanoscale phospholipid bilayers which can self-assemble integral membrane proteins for biophysical, enzymatic or structural investigations. This means for rendering membrane proteins soluble at the single molecule level offers a
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_dedup___::9f6b665fd294b7651f15b4b769d590ca
https://doi.org/10.1016/j.febslet.2009.10.024
https://doi.org/10.1016/j.febslet.2009.10.024
Publikováno v:
Journal of Biological Chemistry. 282:14875-14881
Nanodiscs are nanometer scale planar membranes of controlled size that are rendered soluble in aqueous solution via an encircling amphipathic membrane scaffold protein "belt" (Bayburt, T. H., Grinkova, Y. V., and Sligar, S. G. (2002) Nano. Lett. 2, 8
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_dedup___::6e3d6d1a6e11af627d310e600d59e976
https://doi.org/10.1074/jbc.m701433200
https://doi.org/10.1074/jbc.m701433200
Publikováno v:
Archives of Biochemistry and Biophysics. 450:215-222
Nanodiscs, phospholipid bilayer assemblies of controlled size, were used to self-assemble bacteriorhodopsin (bR) into single trimers. Self-assembly at optimal bR to Nanodisc and phospholipid stoichiometry yielded particles containing three bR molecul
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_dedup___::99598d4f722ba95fcc8c070f1b9119c2
https://doi.org/10.1016/j.abb.2006.03.013
https://doi.org/10.1016/j.abb.2006.03.013
Publikováno v:
Nano Letters. 2:853-856
Nanoparticulate phospholipid bilayer disks were assembled from phospholipid and a class of amphipathic helical proteins termed membrane scaffold proteins (MSP). Several different MSPs were produced in high yield using a synthetic gene and a heterolog
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_________::0b2f94327c66997e343f217b576e96c2
https://doi.org/10.1021/nl025623k
https://doi.org/10.1021/nl025623k
Autor:
Timothy H. Bayburt, Stephen G. Sligar
Publikováno v:
Proceedings of the National Academy of Sciences. 99:6725-6730
The architecture of membrane proteins in their native environment of the phospholipid bilayer is critical for understanding physiological function, but has been difficult to realize experimentally. In this communication we describe the incorporation
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_dedup___::db836e607f851ab9a2a044b65e8436c4
https://doi.org/10.1073/pnas.062565599
https://doi.org/10.1073/pnas.062565599