Zobrazeno 1 - 10
of 59
pro vyhledávání: '"Timothy A Vickers"'
Autor:
Timothy A Vickers, Stanley T Crooke
Publikováno v:
PLoS ONE, Vol 11, Iss 8, p e0161930 (2016)
Protein-nucleic acid interactions play a crucial role in the regulation of diverse biological processes. Elucidating the roles that protein-nucleic acid complexes play in the regulation of transcription, translation, DNA replication, repair and recom
Externí odkaz:
https://doaj.org/article/f3b1bc87cb654ba7b7b5cb800ffba823
Publikováno v:
PLoS ONE, Vol 9, Iss 10, p e110615 (2014)
A new strategy for identifying potent RNase H-dependent antisense oligonucleotides (ASOs) is presented. Our analysis of the human transcriptome revealed that a significant proportion of genes contain unique repeated sequences of 16 or more nucleotide
Externí odkaz:
https://doaj.org/article/96ce270b7c0e48a58c215806e264aeed
Autor:
Timothy A Vickers, Stanley T Crooke
Publikováno v:
PLoS ONE, Vol 9, Iss 10, p e108625 (2014)
Antisense oligonucleotides (ASOs) are most commonly designed to reduce targeted RNA via RNase H1-dependent degradation. In this paper we demonstrate that cellular proteins can compete for sites targeted by RNase H1-dependent ASOs. We further show tha
Externí odkaz:
https://doaj.org/article/3e2c841fcdf541a085ac643d5b7f6fe1
Publikováno v:
PLoS ONE, Vol 9, Iss 7, p e101752 (2014)
To better understand the factors that influence the activity and specificity of antisense oligonucleotides (ASOs), we designed a minigene encoding superoxide dismutase 1 (SOD-1) and cloned the minigene into vectors for T7 transcription of pre-mRNA an
Externí odkaz:
https://doaj.org/article/2c773100b2994ebfb9f9401e512d914e
Publikováno v:
PLoS Biology, Vol 5, Iss 4, p e73 (2007)
Several strategies have been pursued to increase the extent of exon 7 inclusion during splicing of SMN2 (survival of motor neuron 2) transcripts, for eventual therapeutic use in spinal muscular atrophy (SMA), a genetic neuromuscular disease. Antisens
Externí odkaz:
https://doaj.org/article/d01d496ad0a54df892c97b21c468eb83
Autor:
Lingdi, Zhang, Karla D, Bernardo, Timothy A, Vickers, Jun, Tian, Xue-Hai, Liang, Stanley T, Crooke
Publikováno v:
Nucleic Acid Therapeutics. 32:280-299
RNase H1-dependent phosphorothioate oligonucleotides (PS-ASOs) have been developed to treat various diseases through specific degradation of target RNAs. Although many factors or features of RNA and PS-ASOs have been demonstrated to affect antisense
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_dedup___::cb03ace940b5cc82808a2687b1c11502
https://doi.org/10.1089/nat.2021.0107
https://doi.org/10.1089/nat.2021.0107
Publikováno v:
Nucleic Acids Research. 50:8107-8126
Non-CpG PS-ASOs can activate the innate immune system, leading to undesired outcomes. This response can vary—in part—as a function of 2′modifications and sequence. Here we investigated the molecular steps involved in the varied effects of PS-AS
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_dedup___::63cebe7fd7d78a595a42e25c1fe51626
https://doi.org/10.1093/nar/gkac618
https://doi.org/10.1093/nar/gkac618
Publikováno v:
Nucleic Acids Research
We recently found that toxic PS-ASOs can cause P54nrb and PSF nucleolar mislocalization in an RNase H1-dependent manner. To better understand the underlying mechanisms of these observations, here we utilize different biochemical approaches to demonst
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_dedup___::7ee7694058fded8dc2a628594e51c9f8
https://doi.org/10.1093/nar/gkab078
https://doi.org/10.1093/nar/gkab078
Publikováno v:
Nucleic Acids Research
Antisense oligonucleotides (ASOs) interact with target RNAs via hybridization to modulate gene expression through different mechanisms. ASO therapeutics are chemically modified and include phosphorothioate (PS) backbone modifications and different ri
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_dedup___::535cef039e2378e29413ec49d0a76658
https://doi.org/10.1093/nar/gkaa299
https://doi.org/10.1093/nar/gkaa299
Autor:
Hong Sun, Stanley T. Crooke, Timothy A. Vickers, Cheryl L De Hoyos, Chih-Wei Hsu, Joshua G Nichols, Xue-hai Liang
Publikováno v:
Nucleic Acids Research
Release of phosphorothioate antisense oligonucleotides (PS-ASOs) from late endosomes (LEs) is a rate-limiting step and a poorly defined process for productive intracellular ASO drug delivery. Here, we examined the role of Golgi-endosome transport, sp
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_dedup___::a156e8c5af7c48930d8bba22c5e38b9b
https://doi.org/10.1093/nar/gkz1171
https://doi.org/10.1093/nar/gkz1171