Zobrazeno 1 - 10
of 14
pro vyhledávání: '"Timo N. Kohler"'
Autor:
Joachim De Jonghe, Tomasz S. Kaminski, David B. Morse, Marcin Tabaka, Anna L. Ellermann, Timo N. Kohler, Gianluca Amadei, Charlotte E. Handford, Gregory M. Findlay, Magdalena Zernicka-Goetz, Sarah A. Teichmann, Florian Hollfelder
Publikováno v:
Nature Communications, Vol 14, Iss 1, Pp 1-18 (2023)
Abstract Droplet microfluidic methods have massively increased the throughput of single-cell sequencing campaigns. The benefit of scale-up is, however, accompanied by increased background noise when processing challenging samples and the overall RNA
Externí odkaz:
https://doaj.org/article/2dff37ca2c9b446281d0fc46cf2e490a
Autor:
Timo N. Kohler, Joachim De Jonghe, Anna L. Ellermann, Ayaka Yanagida, Michael Herger, Erin M. Slatery, Antonia Weberling, Clara Munger, Katrin Fischer, Carla Mulas, Alex Winkel, Connor Ross, Sophie Bergmann, Kristian Franze, Kevin Chalut, Jennifer Nichols, Thorsten E. Boroviak, Florian Hollfelder
Publikováno v:
Nature Communications, Vol 14, Iss 1, Pp 1-19 (2023)
Abstract Biomechanical cues are instrumental in guiding embryonic development and cell differentiation. Understanding how these physical stimuli translate into transcriptional programs will provide insight into mechanisms underlying mammalian pre-imp
Externí odkaz:
https://doaj.org/article/19d37a4e6ae24ca8b14f83a6a064a669
Publikováno v:
STAR Protocols, Vol 4, Iss 2, Pp 102333- (2023)
Summary: Within the peri-portal region of the adult liver, portal fibroblasts exist in close proximity to epithelial ductal/cholangiocyte cells. However, the cellular interactions between them are poorly understood. Here, we provide two co-culture te
Externí odkaz:
https://doaj.org/article/b18dbcef0c454e0f98bc0ad952eeeecc
Autor:
Timo N Kohler, Magdalena Schindler, Anna L. Ellermann, Dylan Siriwardena, Florian Hollfelder, Erin Slatery, Clara Munger, Thorsten Boroviak
Publikováno v:
Stem Cell Reports
Summary Human periimplantation development requires the transformation of the naive pluripotent epiblast into a polarized epithelium. Lumenogenesis plays a critical role in this process, as the epiblast undergoes rosette formation and lumen expansion
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_dedup___::147f0d63165d48a83852190a79672ddb
http://europepmc.org/articles/PMC8185981
http://europepmc.org/articles/PMC8185981
Autor:
Timo N. Kohler, Joachim De Jonghe, Anna L. Ellerman, Ayaka Yanagida, Michael Herger, Erin M. Slatery, Katrin Fischer, Carla Mulas, Alex Winkel, Connor Ross, Sophie Bergmann, Kristian Franze, Kevin Chalut, Jennifer Nichols, Thorsten E. Boroviak, Florian Hollfelder
Biomechanical cues are instrumental in guiding embryonic development and cell differentiation. Understanding how these physical stimuli translate into transcriptional programs could provide insight into mechanisms underlying mammalian pre-implantatio
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_________::c741f38e1670e171ebf6a355c63c9f57
https://doi.org/10.1101/2022.03.13.484158
https://doi.org/10.1101/2022.03.13.484158
Autor:
Sophie Bergmann, Christopher A. Penfold, Erin Slatery, Dylan Siriwardena, Charis Drummer, Stephen Clark, Stanley E. Strawbridge, Keiko Kishimoto, Alice Vickers, Mukul Tewary, Timo N. Kohler, Florian Hollfelder, Wolf Reik, Erika Sasaki, Rüdiger Behr, Thorsten E. Boroviak
Publikováno v:
Nature
Gastrulation controls the emergence of cellular diversity and axis patterning in the early embryo. In mammals, this transformation is orchestrated by dynamic signalling centres at the interface of embryonic and extraembryonic tissues1-3. Elucidating
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_dedup___::84c865ba8270fb8211dd8935ed4b1598
Autor:
Fredrik Salmen, Joachim De Jonghe, Tomasz S. Kaminski, Anna Alemany, Guillermo E. Parada, Joe Verity-Legg, Ayaka Yanagida, Timo N. Kohler, Nicholas Battich, Floris van den Brekel, Anna L. Ellermann, Alfonso Martinez Arias, Jennifer Nichols, Martin Hemberg, Florian Hollfelder, Alexander van Oudenaarden
Publikováno v:
Nature Biotechnology
Nature Biotechnology. Nature Publishing Group
Nature Biotechnology. Nature Publishing Group
Data de publicació electrònica: 27-06-2022 Most methods for single-cell transcriptome sequencing amplify the termini of polyadenylated transcripts, capturing only a small fraction of the total cellular transcriptome. This precludes the detection of
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_dedup___::a0147bc99e5a4a2ab40ea94e46430701
https://pubmed.ncbi.nlm.nih.gov/35927479
https://pubmed.ncbi.nlm.nih.gov/35927479
Autor:
J. Nichols, F. van den Brekel, J. Verity-Legg, Timo N Kohler, J. De Jonghe, Anna Alemany, Nico Battich, A. Yanagida, Martin Hemberg, Florian Hollfelder, T. S. Kaminski, Alfonso Martinez Arias, A. L. Ellermann, A van Oudenaarden, Guillermo E. Parada, Fredrik Salmén
In recent years, single-cell transcriptome sequencing has revolutionized biology, allowing for the unbiased characterization of cellular subpopulations. However, most methods amplify the termini of polyadenylated transcripts capturing only a small fr
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_________::e67f43959d00c929d6a8bcc363280e8a
https://doi.org/10.1101/2021.09.15.460240
https://doi.org/10.1101/2021.09.15.460240
Autor:
Neil C. Henderson, Richard Butler, Clare Pacini, Timo N Kohler, Ross Dobie, Meritxell Huch, Bernhard Strauss, Anna M. Dowbaj, John R. Wilson-Kanamori, Olga Sarlidou, Florian Hollfelder, Lucía Cordero-Espinoza, Palle Serup
In the homeostatic liver, ductal cells intermingle with a microenvironment of endothelial and mesenchymal cells to form the functional unit of the portal tract. Ductal cells proliferate rarely in homeostasis but do so transiently after tissue injury
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_________::adeab6ee90ca48402638725d013b12bd
https://doi.org/10.1101/2020.09.21.306258
https://doi.org/10.1101/2020.09.21.306258
Autor:
Carla Mulas, Chibeza C. Agley, Florian Hollfelder, Andrew C Hodgson, Timo N Kohler, Kevin J. Chalut, Austin Smith
Publikováno v:
bioRxiv
Combining live imaging with the ability to retrieve individual cells of interest remains a technical challenge. These combined methods are of particular interest when studying highly dynamic or transient, asynchronous or heterogeneous cell biological
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_dedup___::88e96a196e91e82085e32a24809dea2e
https://doi.org/10.1101/2020.02.17.952689
https://doi.org/10.1101/2020.02.17.952689