Competition between two HUSH complexes orchestrates the immune response to retroelement invasion.

Autor: Danac JMC; The Gurdon Institute, University of Cambridge, Tennis Court Road, Cambridge CB2 1QN, UK; Department of Biochemistry, University of Cambridge, Tennis Court Road, Cambridge CB2 1QN, UK., Matthews RE; The Gurdon Institute, University of Cambridge, Tennis Court Road, Cambridge CB2 1QN, UK; Department of Biochemistry, University of Cambridge, Tennis Court Road, Cambridge CB2 1QN, UK., Gungi A; The Gurdon Institute, University of Cambridge, Tennis Court Road, Cambridge CB2 1QN, UK; Department of Biochemistry, University of Cambridge, Tennis Court Road, Cambridge CB2 1QN, UK., Qin C; The Gurdon Institute, University of Cambridge, Tennis Court Road, Cambridge CB2 1QN, UK; Department of Biochemistry, University of Cambridge, Tennis Court Road, Cambridge CB2 1QN, UK., Parsons H; Department of Medicine, Cambridge Institute for Medical Research, Addenbrooke's Hospital, Hills Road, Cambridge CB2 0XY, UK., Antrobus R; Department of Medicine, Cambridge Institute for Medical Research, Addenbrooke's Hospital, Hills Road, Cambridge CB2 0XY, UK., Timms RT; Cambridge Institute of Therapeutic Immunology and Infectious Disease, Jeffrey Cheah Biomedical Centre, Cambridge CB2 0AW, UK., Tchasovnikarova IA; The Gurdon Institute, University of Cambridge, Tennis Court Road, Cambridge CB2 1QN, UK; Department of Biochemistry, University of Cambridge, Tennis Court Road, Cambridge CB2 1QN, UK. Electronic address: it257@cam.ac.uk.

Publikováno v: Molecular cell [Mol Cell] 2024 Aug 08; Vol. 84 (15), pp. 2870-2881.e5. Date of Electronic Publication: 2024 Jul 15.

Mechanism of Ψ-Pro/C-degron recognition by the CRL2 ^FEM1B ubiquitin ligase.

Autor: Chen X; MOE Key Laboratory for Cellular Dynamics, Division of Life Sciences and Medicine, University of Science and Technology of China, Hefei, 230027, PR China.; Center for Advanced Interdisciplinary Science and Biomedicine of IHM, Division of Life Sciences and Medicine, University of Science and Technology of China, Hefei, 230027, PR China., Raiff A; The Mina and Everard Goodman Faculty of Life Sciences, Bar-Ilan University, Ramat-Gan, 5290002, Israel., Li S; MOE Key Laboratory for Cellular Dynamics, Division of Life Sciences and Medicine, University of Science and Technology of China, Hefei, 230027, PR China., Guo Q; MOE Key Laboratory for Cellular Dynamics, Division of Life Sciences and Medicine, University of Science and Technology of China, Hefei, 230027, PR China., Zhang J; MOE Key Laboratory for Cellular Dynamics, Division of Life Sciences and Medicine, University of Science and Technology of China, Hefei, 230027, PR China., Zhou H; MOE Key Laboratory for Cellular Dynamics, Division of Life Sciences and Medicine, University of Science and Technology of China, Hefei, 230027, PR China., Timms RT; Cambridge Institute of Therapeutic Immunology and Infectious Disease, Department of Medicine, University of Cambridge, Cambridge, UK., Yao X; MOE Key Laboratory for Cellular Dynamics, Division of Life Sciences and Medicine, University of Science and Technology of China, Hefei, 230027, PR China., Elledge SJ; Division of Genetics, Department of Medicine, Howard Hughes Medical Institute, Brigham and Women's Hospital, Harvard Medical School, Boston, MA, 02115, USA., Koren I; The Mina and Everard Goodman Faculty of Life Sciences, Bar-Ilan University, Ramat-Gan, 5290002, Israel. itay.koren@biu.ac.il., Zhang K; MOE Key Laboratory for Cellular Dynamics, Division of Life Sciences and Medicine, University of Science and Technology of China, Hefei, 230027, PR China. kmzhang@ustc.edu.cn., Xu C; MOE Key Laboratory for Cellular Dynamics, Division of Life Sciences and Medicine, University of Science and Technology of China, Hefei, 230027, PR China. xuchaor@ustc.edu.cn.; Center for Advanced Interdisciplinary Science and Biomedicine of IHM, Division of Life Sciences and Medicine, University of Science and Technology of China, Hefei, 230027, PR China. xuchaor@ustc.edu.cn.

Publikováno v: Nature communications [Nat Commun] 2024 Apr 26; Vol. 15 (1), pp. 3558. Date of Electronic Publication: 2024 Apr 26.

Elucidation of E3 ubiquitin ligase specificity through proteome-wide internal degron mapping.

Autor: Zhang Z, Sie B, Chang A, Leng Y, Nardone C, Timms RT, Elledge SJ

Publikováno v: Molecular cell [Mol Cell] 2023 Nov 16; Vol. 83 (22), pp. 4191-4192. Date of Electronic Publication: 2023 Nov 16.

High-throughput, targeted MHC class I immunopeptidomics using a functional genetics screening platform.

Autor: Bruno PM; Department of Genetics, Harvard Medical School and Division of Genetics, Brigham and Women's Hospital, Boston, MA, USA.; Howard Hughes Medical Institute, Chevy Chase, MD, USA., Timms RT; Department of Genetics, Harvard Medical School and Division of Genetics, Brigham and Women's Hospital, Boston, MA, USA.; Howard Hughes Medical Institute, Chevy Chase, MD, USA.; Cambridge Institute of Therapeutic Immunology and Infectious Disease, Jeffrey Cheah Biomedical Centre, Cambridge Biomedical Campus, University of Cambridge, Cambridge, UK., Abdelfattah NS; Department of Genetics, Harvard Medical School and Division of Genetics, Brigham and Women's Hospital, Boston, MA, USA.; Howard Hughes Medical Institute, Chevy Chase, MD, USA., Leng Y; Department of Genetics, Harvard Medical School and Division of Genetics, Brigham and Women's Hospital, Boston, MA, USA.; Howard Hughes Medical Institute, Chevy Chase, MD, USA., Lelis FJN; Department of Medicine, Division of Allergy and Immunology, Division of Genetics, Brigham and Women's Hospital, Harvard Medical School, Boston, MA, USA., Wesemann DR; Department of Medicine, Division of Allergy and Immunology, Division of Genetics, Brigham and Women's Hospital, Harvard Medical School, Boston, MA, USA.; Massachusetts Consortium on Pathogen Readiness, Boston, MA, USA., Yu XG; Ragon Institute of MGH, MIT and Harvard, Cambridge, MA, USA.; Infectious Disease Division, Brigham and Women's Hospital, Boston, MA, USA., Elledge SJ; Department of Genetics, Harvard Medical School and Division of Genetics, Brigham and Women's Hospital, Boston, MA, USA. selledge@genetics.med.harvard.edu.; Howard Hughes Medical Institute, Chevy Chase, MD, USA. selledge@genetics.med.harvard.edu.

Publikováno v: Nature biotechnology [Nat Biotechnol] 2023 Jul; Vol. 41 (7), pp. 980-992. Date of Electronic Publication: 2023 Jan 02.

Germline-encoded amino acid-binding motifs drive immunodominant public antibody responses.

Autor: Shrock EL; Department of Genetics, Harvard Medical School, Boston, MA 02115, USA.; Division of Genetics, Department of Medicine, Howard Hughes Medical Institute, Brigham and Women's Hospital, Boston, MA 02115, USA.; Program in Biological and Biomedical Sciences, Harvard University, Boston, MA 02115, USA., Timms RT; Cambridge Institute of Therapeutic Immunology and Infectious Disease, Jeffrey Cheah Biomedical Centre, Cambridge Biomedical Campus, University of Cambridge, Cambridge, UK., Kula T; Department of Genetics, Harvard Medical School, Boston, MA 02115, USA.; Division of Genetics, Department of Medicine, Howard Hughes Medical Institute, Brigham and Women's Hospital, Boston, MA 02115, USA.; Program in Biological and Biomedical Sciences, Harvard University, Boston, MA 02115, USA., Mena EL; Department of Genetics, Harvard Medical School, Boston, MA 02115, USA.; Division of Genetics, Department of Medicine, Howard Hughes Medical Institute, Brigham and Women's Hospital, Boston, MA 02115, USA., West AP Jr; Division of Biology and Biological Engineering, California Institute of Technology, Pasadena, CA 91125, USA., Guo R; Division of Infectious Disease, Department of Medicine, Brigham and Women's Hospital, Harvard Medical School, Boston, MA 02115, USA.; Department of Microbiology, Harvard Medical School, Boston, MA 02115, USA.; Broad Institute of Harvard and MIT, Cambridge, MA, 02142, USA., Lee IH; Center for Systems Biology, Department of Radiology, Massachusetts General Hospital and Harvard Medical School, Boston, MA 02114, USA., Cohen AA; Division of Biology and Biological Engineering, California Institute of Technology, Pasadena, CA 91125, USA., McKay LGA; National Emerging Infectious Diseases Laboratories, Boston University School of Medicine, Boston University, Boston, MA 02118, USA., Bi C; Division of Allergy and Immunology, Division of Genetics, Department of Medicine, Brigham and Women's Hospital, Harvard Medical School, Boston, MA 02115, USA.; Massachusetts Consortium on Pathogen Readiness, Boston, MA 02115, USA., Leng Y; Department of Genetics, Harvard Medical School, Boston, MA 02115, USA.; Division of Genetics, Department of Medicine, Howard Hughes Medical Institute, Brigham and Women's Hospital, Boston, MA 02115, USA., Fujimura E; Department of Genetics, Harvard Medical School, Boston, MA 02115, USA.; Division of Genetics, Department of Medicine, Howard Hughes Medical Institute, Brigham and Women's Hospital, Boston, MA 02115, USA., Horns F; Department of Bioengineering, Department of Applied Physics, Chan Zuckerberg Biohub and Stanford University, Stanford, CA 94305, USA., Li M; Department of Genetics, Harvard Medical School, Boston, MA 02115, USA.; Division of Genetics, Department of Medicine, Howard Hughes Medical Institute, Brigham and Women's Hospital, Boston, MA 02115, USA., Wesemann DR; Broad Institute of Harvard and MIT, Cambridge, MA, 02142, USA.; Division of Allergy and Immunology, Division of Genetics, Department of Medicine, Brigham and Women's Hospital, Harvard Medical School, Boston, MA 02115, USA.; Massachusetts Consortium on Pathogen Readiness, Boston, MA 02115, USA.; Ragon Institute of MGH, MIT, and Harvard, Cambridge, MA, 02139 USA., Griffiths A; National Emerging Infectious Diseases Laboratories, Boston University School of Medicine, Boston University, Boston, MA 02118, USA., Gewurz BE; Division of Infectious Disease, Department of Medicine, Brigham and Women's Hospital, Harvard Medical School, Boston, MA 02115, USA.; Department of Microbiology, Harvard Medical School, Boston, MA 02115, USA.; Broad Institute of Harvard and MIT, Cambridge, MA, 02142, USA.; Graduate Program in Virology, Division of Medical Sciences, Harvard Medical School, Boston, MA 02115, USA., Bjorkman PJ; Division of Biology and Biological Engineering, California Institute of Technology, Pasadena, CA 91125, USA., Elledge SJ; Department of Genetics, Harvard Medical School, Boston, MA 02115, USA.; Division of Genetics, Department of Medicine, Howard Hughes Medical Institute, Brigham and Women's Hospital, Boston, MA 02115, USA.

Publikováno v: Science (New York, N.Y.) [Science] 2023 Apr 07; Vol. 380 (6640), pp. eadc9498. Date of Electronic Publication: 2023 Apr 07.