Zobrazeno 1 - 10
of 62
pro vyhledávání: '"Tim L. Sit"'
Autor:
Irene N. Gentzel, Chan Ho Park, Maria Bellizzi, Guiqing Xiao, Kiran R. Gadhave, Colin Murphree, Qin Yang, Jonathan LaMantia, Margaret G. Redinbaugh, Peter Balint-Kurti, Tim L. Sit, Guo-Liang Wang
Publikováno v:
Plant Methods, Vol 16, Iss 1, Pp 1-9 (2020)
Abstract Background The Clustered Regularly Interspaced Short Palindromic Repeats (CRISPR)/Cas9 system has become a powerful tool for functional genomics in plants. The RNA-guided nuclease can be used to not only generate precise genomic mutations, b
Externí odkaz:
https://doaj.org/article/37f400e2ce4849c38134d8506c342d3a
Autor:
Laura J. Kraft, Tim L. Sit, Lauren M. Diepenbrock, Hamid Ashrafi, Rishi Aryal, Gina E. Fernandez, Hannah J. Burrack
Publikováno v:
Frontiers in Ecology and Evolution, Vol 9 (2021)
The feeding habits of adult Brachycera are understudied and may provide important context for understanding invasive pest biology, as with the polyphagous small fruit pest Drosophila suzukii. We developed molecular methods to study adult D. suzukii g
Externí odkaz:
https://doaj.org/article/5d05146e072d4b0d9d2fc850350cf944
Publikováno v:
MethodsX, Vol 6, Iss , Pp 2720-2728 (2019)
A fluorescence in situ hybridization (FISH) protocol was developed for nematodes in which nucleic acid probes are introduced within the organism via electroporation. This modification of existing FISH protocols removes numerous chemical wash steps, a
Externí odkaz:
https://doaj.org/article/ce0947c7d717410793f1af9215e5abd9
Publikováno v:
Molecular Plant-Microbe Interactions, Vol 21, Iss 7, Pp 879-890 (2008)
The cell-to-cell movement of Turnip crinkle virus (TCV) in Nicotiana benthamiana requires the presence of its coat protein (CP), a known suppressor of RNA silencing. RNA transcripts of a TCV construct containing a reporter gene (green fluorescent pro
Externí odkaz:
https://doaj.org/article/d4c8b3c20a9a4e8bbe61c475795b271e
Autor:
Tahira Pirzada, Antoine Affokpon, Richard H. Guenther, Reny Mathew, Sachin Agate, Aitana Blevins, Medwick V. Byrd, Tim L. Sit, Stephen R. Koenning, Eric L. Davis, Lokendra Pal, Charles H. Opperman, Saad A. Khan
Publikováno v:
Nature Food. 4:148-159
Sustainable practices that reduce food loss are essential for enhancing global food security. We report a ‘wrap and plant’ seed treatment platform to protect crops from soil-borne pathogens. Developed from the abundantly available wastes of banan
Autor:
Colin Murphree, Maria Bellizzi, Qin Yang, Peter J. Balint-Kurti, Guiqing Xiao, Tim L. Sit, Guo-Liang Wang, Chan Ho Park, Kiran R. Gadhave, Irene N. Gentzel, Margaret G. Redinbaugh, Jonathan LaMantia
Publikováno v:
Plant Methods, Vol 16, Iss 1, Pp 1-9 (2020)
Plant Methods
Plant Methods
Background The Clustered Regularly Interspaced Short Palindromic Repeats (CRISPR)/Cas9 system has become a powerful tool for functional genomics in plants. The RNA-guided nuclease can be used to not only generate precise genomic mutations, but also t
Publikováno v:
Phytopathology. 112(4)
Take-all root rot is a disease of ultradwarf bermudagrass putting greens caused by Gaeumannomyces graminis (Gg), Gaeumannomyces sp. (Gx), Gaeumannomyces graminicola (Ggram), Candidacolonium cynodontis (Cc), and Magnaporthiopsis cynodontis (Mc). Many
Autor:
Hamid Ashrafi, Laura J. Kraft, Gina E. Fernandez, Hannah J. Burrack, Tim L. Sit, Rishi Aryal, Lauren M Diepenbrock
Publikováno v:
Frontiers in Ecology and Evolution, Vol 9 (2021)
The feeding habits of adult Brachycera are understudied and may provide important context for understanding invasive pest biology, as with the polyphagous small fruit pest Drosophila suzukii. We developed molecular methods to study adult D. suzukii g
Autor:
Saad A. Khan, Tahira Pirzada, Barbara V. Farias, Tim L. Sit, Charles H. Opperman, Reny Mathew
Publikováno v:
ACS Sustainable Chemistry & Engineering. 7:19848-19856
Ineffective delivery of pesticides leads to multiple application cycles of active ingredients (AIs), resulting in increased cost while endangering the environment via soil, water, and air contamina...
Publikováno v:
MethodsX, Vol 6, Iss, Pp 2720-2728 (2019)
MethodsX
MethodsX
Graphical abstract
A fluorescence in situ hybridization (FISH) protocol was developed for nematodes in which nucleic acid probes are introduced within the organism via electroporation. This modification of existing FISH protocols removes numerou
A fluorescence in situ hybridization (FISH) protocol was developed for nematodes in which nucleic acid probes are introduced within the organism via electroporation. This modification of existing FISH protocols removes numerou