Zobrazeno 1 - 10
of 198
pro vyhledávání: '"Tim E, Cawston"'
Publikováno v:
Arthritis & Rheumatology. 66:979-989
Objective To use a novel computational approach to examine the molecular pathways involved in cartilage breakdown and to use computer simulation to test possible interventions for reducing collagen release. Methods We constructed a computational mode
Autor:
David Young, Andrew D. Rowan, M Elias, Gareth I Kitson, Gary J. Litherland, Tim E. Cawston, Wang Hui
Publikováno v:
Annals of the Rheumatic Diseases. 71:455-462
ObjectivesTo investigate the effect of leptin on cartilage destruction.MethodsCollagen release was assessed in bovine cartilage explant cultures, while collagenolytic and gelatinolytic activities in culture supernatants were determined by bioassay an
Autor:
Matthew Jefferson, Tim E. Cawston, M Elias, Gary J. Litherland, Wang Hui, Matt J. Barter, David Young, Andrew D. Rowan
Publikováno v:
Rheumatology. 49:2043-2053
Objectives To determine the effects and mechanism of action of lithium chloride (LiCl) on cartilage destruction induced by the pro-inflammatory cytokines IL-1, IL-1 + oncostatin M and TNF-α. Methods The release of collagen was assessed in bovine car
Autor:
Tim E. Cawston, Rachel L. Lakey
Publikováno v:
Rheumatology. 48:1208-1212
Objective To investigate the effect of SSZ on the release of GAG and collagen fragments from bovine nasal cartilage and MMP and ADAMTS (a disintegrin and metalloproteinase domain with thrombospondin motifs) proteinases from human articular chondrocyt
Autor:
Tim E. Cawston
Publikováno v:
Nature Protocols. 4:286-290
This protocol describes how to purify and radiolabel collagen for use as a substrate to assay collagenolytic members of the matrix metalloproteinases (MMPs). This assay measures enzymes that specifically cleave native triple helical collagen. After i
Autor:
Rachel L. Lakey, Andrew D. Rowan, John D. Isaacs, Catharien M. U. Hilkens, Tanya G. Morgan, Tim E. Cawston
Publikováno v:
Rheumatology. 48:502-507
Objective. Dendritic cells (DCs) are enriched in RA synovium and have been implicated in the pathogenesis of RA primarily through their ability to present autoantigen and activate T cells. However, whether DCs play an effector role in cartilage destr
Autor:
GE Johnson, Ian Forrest, James Lordan, T Small, Desmond M. Murphy, Chris Ward, Andrew J. Fisher, Debbie Jones, Tim E. Cawston, James J. Egan, Paul A. Corris
Publikováno v:
The Journal of Heart and Lung Transplantation. 27:1210-1216
Background The bronchial epithelium is a source of mediators that may play a role in the airway inflammation and remodeling of post-transplant obliterative bronchiolitis (OB). Traditional strategies have failed to have an impact on OB. Recent studies
Autor:
Timothy Robson, Tim E. Cawston, Rachel L. Lakey, D Jones, Gary J. Litherland, Craig Dixon, Andrew D. Rowan, David Young
Publikováno v:
Journal of Biological Chemistry. 283:14221-14229
The phosphatidylinositol 3-kinase (PI3K) signaling pathway has emerged as a major regulator of cellular functions and has been implicated in several pathologies involving remodeling of extracellular matrix (ECM). The end stage of inflammatory joint d
Autor:
Jim J. Egan, James Lordan, GE Johnson, Andrew J. Fisher, Debbie Jones, Tim E. Cawston, Paul A. Corris, T Small, Ian Forrest, Desmond M. Murphy, Chris Ward
Publikováno v:
American Journal of Physiology-Lung Cellular and Molecular Physiology. 294:L592-L599
Obliterative bronchiolitis (OB), the major cause of chronic lung allograft dysfunction, is characterized by airway neutrophilia, inflammation, and remodeling, with progressive fibroproliferation and obliteration of small airways that ultimately leads
Autor:
Matt J. Barter, Andrew D. Rowan, John H. Robinson, David Young, Ian M. Clark, Simon T. Donell, Wang Hui, C. Darrah, Qian Zhang, Gary J. Litherland, Tim E. Cawston, Rose K Davidson
Publikováno v:
Annals of the Rheumatic Diseases. 67:1633-1641
Objectives: To characterise the catabolic response of osteoarthritic chondrocytes to Toll-like receptor (TLR) ligands. Methods: Induction of the collagenases, matrix metalloproteinase (MMP)1 and MMP13, by TLR ligands was assessed in chondrocytes by r