Zobrazeno 1 - 10
of 43
pro vyhledávání: '"Tim C. Huffaker"'
Autor:
Elisabeth A Geyer, Alexander Burns, Beth A Lalonde, Xuecheng Ye, Felipe-Andres Piedra, Tim C Huffaker, Luke M Rice
Publikováno v:
eLife, Vol 4 (2015)
Microtubule dynamic instability depends on the GTPase activity of the polymerizing αβ-tubulin subunits, which cycle through at least three distinct conformations as they move into and out of microtubules. How this conformational cycle contributes t
Externí odkaz:
https://doaj.org/article/c2d34aef84494b51a69e725fc14c5c3d
Autor:
Laura-Oana Albulescu, Nevin Sabet, Mohanram Gudipati, Nicholas Stepankiw, Zane J Bergman, Tim C Huffaker, Jeffrey A Pleiss
Publikováno v:
PLoS Genetics, Vol 8, Iss 3, p e1002530 (2012)
Here we present the development and implementation of a genome-wide reverse genetic screen in the budding yeast, Saccharomyces cerevisiae, that couples high-throughput strain growth, robotic RNA isolation and cDNA synthesis, and quantitative PCR to a
Externí odkaz:
https://doaj.org/article/93b1f5a477324f42b8fcf6f0f117ea9c
Publikováno v:
Molecular Biology of the Cell
Loss of the dynein inhibitor She1 causes increased rates of microtubule detachment from the yeast spindle pole body. The molecular nature of these detachment events is characterized, and it is shown that their frequency depends on the way in which mi
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_dedup___::ffa39f78439a5794613fb0450213e0be
https://doi.org/10.1091/mbc.e12-03-0223
https://doi.org/10.1091/mbc.e12-03-0223
Autor:
Xuecheng Ye, Alexander Burns, Luke M. Rice, Tim C. Huffaker, Elisabeth A. Geyer, Beth A. Lalonde, Felipe-Andres Piedra
Publikováno v:
eLife, Vol 4 (2015)
eLife
eLife
Microtubule dynamic instability depends on the GTPase activity of the polymerizing αβ-tubulin subunits, which cycle through at least three distinct conformations as they move into and out of microtubules. How this conformational cycle contributes t
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_dedup___::a02bde3de7ae9705a7a33b7c90db2ad7
https://doi.org/10.7554/elife.10113
https://doi.org/10.7554/elife.10113
Autor:
Baoying Huang, Tim C. Huffaker
Publikováno v:
The Journal of Cell Biology
Attachment of chromosomes to the mitotic spindle has been proposed to require dynamic microtubules that randomly search three-dimensional space and become stabilized upon capture by kinetochores. In this study, we test this model by examining chromos
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_dedup___::d3f28c24c48656d2a9ef431fb73f95c6
https://doi.org/10.1083/jcb.200606021
https://doi.org/10.1083/jcb.200606021
Publikováno v:
The Journal of Cell Biology
Microtubules and actin filaments interact and cooperate in many processes in eukaryotic cells, but the functional implications of such interactions are not well understood. In the yeast Saccharomyces cerevisiae, both cytoplasmic microtubules and acti
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_dedup___::ed50a4169541e355a2e3366513c1ac90
http://europepmc.org/articles/PMC2172944
http://europepmc.org/articles/PMC2172944
Publikováno v:
Current Opinion in Microbiology. 5:564-574
New evidence that cortical actin patches and the endocytic machinery share components supports the idea that actin patches are in fact transient membrane coats at the initial stage of endocytosis. Recent studies of actin cables have identified formin
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_dedup___::64a01a2d1244a8e902f767f10e881d9d
https://doi.org/10.1016/s1369-5274(02)00369-7
https://doi.org/10.1016/s1369-5274(02)00369-7
Autor:
Christine T Brew, Tim C. Huffaker
Publikováno v:
Genetics. 162:1079-1089
Stu1p is a microtubule-associated protein required for spindle assembly. In this article we show that the temperature-sensitive stu1-5 allele is synthetically lethal in combination with ubp3, gim1-gim5, and kem1 mutations. The primary focus of this a
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_dedup___::0045c81af4066a902c966e240b3f0906
https://doi.org/10.1093/genetics/162.3.1079
https://doi.org/10.1093/genetics/162.3.1079
Publikováno v:
Molecular Biology of the Cell. 13:1881-1892
Formation of the bipolar mitotic spindle relies on a balance of forces acting on the spindle poles. The primary outward force is generated by the kinesin-related proteins of the BimC family that cross-link antiparallel interpolar microtubules and sli
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_dedup___::dc5a56d161d6b71b49265becb3f3fd43
https://doi.org/10.1091/mbc.01-09-0458
https://doi.org/10.1091/mbc.01-09-0458