Engineering an Anti-Transferrin Receptor ScFv for pH-Sensitive Binding Leads to Increased Intracellular Accumulation.

Autor: Tillotson BJ; University of Wisconsin-Madison, Dept. of Chemical and Biological Engineering, Madison, WI, United States of America., Goulatis LI; University of Wisconsin-Madison, Dept. of Chemical and Biological Engineering, Madison, WI, United States of America., Parenti I; University of Wisconsin-Madison, Dept. of Chemical and Biological Engineering, Madison, WI, United States of America., Duxbury E; University of Wisconsin-Madison, Dept. of Chemical and Biological Engineering, Madison, WI, United States of America., Shusta EV; University of Wisconsin-Madison, Dept. of Chemical and Biological Engineering, Madison, WI, United States of America.

Publikováno v: PloS one [PLoS One] 2015 Dec 29; Vol. 10 (12), pp. e0145820. Date of Electronic Publication: 2015 Dec 29 (Print Publication: 2015).

Yeast Display-Based Antibody Affinity Maturation Using Detergent-Solubilized Cell Lysates.

Autor: Tillotson BJ; Department of Chemical and Biological Engineering, University of Wisconsin-Madison, 3631 Engineering Hall, 1415 Engineering Drive, Madison, WI, 53706, USA., Lajoie JM, Shusta EV

Publikováno v: Methods in molecular biology (Clifton, N.J.) [Methods Mol Biol] 2015; Vol. 1319, pp. 65-78.

Antibody affinity maturation using yeast display with detergent-solubilized membrane proteins as antigen sources.

Autor: Tillotson BJ; Department of Chemical and Biological Engineering, University of Wisconsin-Madison, 1415 Engineering Dr., Madison, WI 53706, USA., de Larrinoa IF, Skinner CA, Klavas DM, Shusta EV

Publikováno v: Protein engineering, design & selection : PEDS [Protein Eng Des Sel] 2013 Feb; Vol. 26 (2), pp. 101-12. Date of Electronic Publication: 2012 Oct 28.