Zobrazeno 1 - 10
of 31
pro vyhledávání: '"Tigst Demeke"'
Publikováno v:
Biology, Vol 11, Iss 2, p 201 (2022)
The number of genetically modified (GM) events for canola, maize, and soybean has been steadily increasing. Real-time PCR is widely used for the detection and quantification of individual GM events. Digital PCR (dPCR) has also been used for absolute
Externí odkaz:
https://doaj.org/article/7cde12388405414db127ed0a9be37344
Autor:
Sung-Jong Lee, Tigst Demeke, Mathieu Dusabenyagasani, Danny Saydak, Daniel Perry, Sean Walkowiak
Publikováno v:
Canadian Journal of Plant Science.
In this study, we report an updated panel of 32 DNA markers used for identification of wheat varieties and assess their performance in the OpenArray and SmartChip high-throughput genotyping systems. While both systems are unique and offer different a
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_________::333a5d8d37b5886dd2c1396970071d49
https://doi.org/10.1139/cjps-2022-0192
https://doi.org/10.1139/cjps-2022-0192
Publikováno v:
Food Analytical Methods. 14:372-379
Low-level detection and quantification of genetically engineered (GE) traits with polymerase chain reaction (PCR) is challenging. For unapproved GE events, any level of detection is not acceptable in some countries because of zero tolerance. Droplet
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_________::696a8a64ac0265a72aed0af2a802d8f4
https://doi.org/10.1007/s12161-020-01889-y
https://doi.org/10.1007/s12161-020-01889-y
Autor:
Monika Eng, Tigst Demeke
Publikováno v:
Biomolecular Detection and Quantification, Vol 15, Iss, Pp 24-29 (2018)
Droplet digital PCR (ddPCR) has been used for absolute quantification of genetically engineered (GE) events. Absolute quantification of GE events by duplex ddPCR requires the use of appropriate primers and probes for target and reference gene sequenc
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_dedup___::dcf8a0f9b40ef5fcb2bcb8d26ef278af
http://www.sciencedirect.com/science/article/pii/S2214753518300019
http://www.sciencedirect.com/science/article/pii/S2214753518300019
Autor:
Tigst Demeke, David Dobnik
Publikováno v:
Analytical and Bioanalytical Chemistry
The number of genetically modified organisms (GMOs) on the market is steadily increasing. Because of regulation of cultivation and trade of GMOs in several countries, there is pressure for their accurate detection and quantification. Today, DNA-based
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_dedup___::53cde7e22f4209190515f3928f12a486
https://doi.org/10.1007/s00216-018-1010-1
https://doi.org/10.1007/s00216-018-1010-1
Publikováno v:
Food Control. 68:105-111
Low level presence of unapproved genetically engineered (GE) materials in non-GE grains has been a challenge for grain importers and exporters and many countries also have regulatory requirements for use and cultivation of GE crops. Digital PCR has b
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_________::b9246a432942d439a041bb58b32f41ec
https://doi.org/10.1016/j.foodcont.2016.03.007
https://doi.org/10.1016/j.foodcont.2016.03.007
Publikováno v:
Food Control. 115:107291
Regulations and labeling laws established by many countries for genetically engineered (GE) events necessitate the development of effective testing methods. Both real-time quantitative PCR (RT-qPCR) and digital PCR (dPCR) have been used for the detec
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_________::3eaeb768e3d756a68cf9f816723528ea
https://doi.org/10.1016/j.foodcont.2020.107291
https://doi.org/10.1016/j.foodcont.2020.107291
Publikováno v:
Journal of AOAC International. 100(2)
Seven commercially available DNA extraction kits were compared with a cetyltrimethylammonium bromide (CTAB)method to determine the suitability of the extracted DNA for RainDrop digital PCR (dPCR) and real-time PCR (RT-PCR) quantification of OXY235 ca
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_dedup___::5165bc850ea248c8578e6fbf3e302cd8
https://pubmed.ncbi.nlm.nih.gov/28118137
https://pubmed.ncbi.nlm.nih.gov/28118137
Autor:
Janice Bamforth, Tigst Demeke, Tom Gräfenhan, Sung-Jong Lee, Ursla Fernando, Michelle Holigroski
Publikováno v:
Food Control. 46:470-474
Real-time PCR is commonly used for quantification of genetically engineered (GE) materials. Digital PCR (dPCR) assays have an advantage over real-time quantitative PCR assays in terms of sensitivity and precision. For meaningful real-time PCR results
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_________::aeff5a6c8be4cf7c34d7fb4c684fdcf0
https://doi.org/10.1016/j.foodcont.2014.06.018
https://doi.org/10.1016/j.foodcont.2014.06.018
Autor:
Sean M. Hemmingsen, Matthew G. Links, Tim J. Dumonceaux, Tom Gräfenhan, Janet E. Hill, Tigst Demeke
Publikováno v:
New Phytologist. 202:542-553
In order to address the hypothesis that seeds from ecologically and geographically diverse plants harbor characteristic epiphytic microbiota, we characterized the bacterial and fungal microbiota associated with Triticum and Brassica seed surfaces. Th
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_________::9ec5022cbfbe680b2019583b9a3355e9
https://doi.org/10.1111/nph.12693
https://doi.org/10.1111/nph.12693