Strategy for Development of Site-Specific Ubiquitin Antibodies

Autor: Ila van Kruijsbergen, Monique P. C. Mulder, Michael Uckelmann, Tibor van Welsem, John de Widt, Aldo Spanjaard, Heinz Jacobs, Farid El Oualid, Huib Ovaa, Fred van Leeuwen

Publikováno v: Frontiers in Chemistry, Vol 8 (2020)

Protein ubiquitination is a key post-translational modification regulating a wide range of biological processes. Ubiquitination involves the covalent attachment of the small protein ubiquitin to a lysine of a protein substrate. In addition to its wel

Externí odkaz: https://doaj.org/article/96f73bcacfac4bcd927a6cd4d515cee0

Decoding the chromatin proteome of a single genomic locus by DNA sequencing.

Autor: Tessy Korthout, Deepani W Poramba-Liyanage, Ila van Kruijsbergen, Kitty F Verzijlbergen, Frank P A van Gemert, Tibor van Welsem, Fred van Leeuwen

Publikováno v: PLoS Biology, Vol 16, Iss 7, p e2005542 (2018)

Transcription, replication, and repair involve interactions of specific genomic loci with many different proteins. How these interactions are orchestrated at any given location and under changing cellular conditions is largely unknown because systema

Externí odkaz: https://doaj.org/article/0074c10b0715470d9cccd82434924638

A UV-Induced Genetic Network Links the RSC Complex to Nucleotide Excision Repair and Shows Dose-Dependent Rewiring

Autor: Rohith Srivas, Thomas Costelloe, Anne-Ruxandra Carvunis, Sovan Sarkar, Erik Malta, Su Ming Sun, Marijke Pool, Katherine Licon, Tibor van Welsem, Fred van Leeuwen, Peter J. McHugh, Haico van Attikum, Trey Ideker

Publikováno v: Cell Reports, Vol 5, Iss 6, Pp 1714-1724 (2013)

Efficient repair of UV-induced DNA damage requires the precise coordination of nucleotide excision repair (NER) with numerous other biological processes. To map this crosstalk, we generated a differential genetic interaction map centered on quantitat

Externí odkaz: https://doaj.org/article/49b48a24b019447baf1e06106fc31587

Inhibition of transcription leads to rewiring of locus-specific chromatin proteomes

Autor: Ila van Kruijsbergen, Tibor van Welsem, Christine E. Cucinotta, Fred van Leeuwen, Tessy Korthout, Deepani W. Poramba-Liyanage, Toshio Tsukiyama, Dris El Atmioui, Huib Ovaa

Publikováno v: Genome Research, 30(4), 635-646. COLD SPRING HARBOR LAB PRESS, PUBLICATIONS DEPT
Genome research, 30(4), 635-646. Cold Spring Harbor Laboratory Press
Genome Res

Transcription of a chromatin template involves the concerted interaction of many different proteins and protein complexes. Analyses of specific factors showed that these interactions change during stress and upon developmental switches. However, how

Externí odkaz: https://explore.openaire.eu/search/publication?articleId=doi_dedup___::fed977d149821cf144d991c9dd339009
https://doi.org/10.1101/gr.256255.119

The histone methyltransferase DOT1L prevents antigen-independent differentiation and safeguards epigenetic identity of CD8(+) T cells

Autor: Teun van den Brand, Tibor van Welsem, Mir Farshid Alemdehy, Joke M. M. den Haan, Sjoerd Hendriks, Jannie Borst, Fred van Leeuwen, Muhammad Aslam, Tessy Korthout, Elzo de Wit, Heinz Jacobs, Cesare Lancini, Eliza Mari Kwesi-Maliepaard, Chelsea M. McLean, Tomasz Ahrends, Hanneke Vlaming, Dieke van Dinther

Publikováno v: Proceedings of the National Academy of Sciences of the United States of America
Proceedings of the National Academy of Sciences of the United States of America, 117(34), 20706-20716. National Academy of Sciences
Kwesi-Maliepaard, E M, Aslam, M A, Alemdehy, M F, Van Den Brand, T V, McLean, C, Vlaming, H, van Welsem, T, Korthout, T, Lancini, C, Hendriks, S, Ahrends, T, van Dinther, D, den Haan, J M M, Borst, J, de Wit, E, van Leeuwen, F & Jacobs, H 2020, ' The histone methyltransferase DOT1L prevents antigen-independent differentiation and safeguards epigenetic identity of CD8 + T cells ', Proceedings of the National Academy of Sciences of the United States of America, vol. 117, no. 34, pp. 20706-20716 . https://doi.org/10.1073/pnas.1920372117
Proceedings of the National Academy of Sciences, 117(34), 20706-20716. NATL ACAD SCIENCES

Significance Cytotoxic CD8+ T cells control infectious diseases as well as cancer. Memory T cells are a subset of CD8+ T cells that have previously encountered and rapidly responded to a specific antigen. Here we identified the epigenetic writer DOT1

Externí odkaz: https://explore.openaire.eu/search/publication?articleId=doi_dedup___::15f4e0b0e06a07eb8799ed142a9ff0b7
https://hdl.handle.net/1887/3184225