Zobrazeno 1 - 10
of 38
pro vyhledávání: '"Thorsten Verch"'
Autor:
Zhifeng Chen, Kara S. Cox, Aimin Tang, Jeanette Roman, Malorie Fink, Robin M. Kaufhold, Liming Guan, Andy Xie, Melissa A. Boddicker, Debra Mcguinness, Xiao Xiao, Hualin Li, Julie M. Skinner, Thorsten Verch, Mary Retzlaff, Kalpit A. Vora
Publikováno v:
BMC Infectious Diseases, Vol 18, Iss 1, Pp 1-11 (2018)
Abstract Background Community-acquired pneumonia is a leading infectious cause of hospitalization. A few vaccines exist to prevent pneumococcal disease in adults, including a pneumococcal polysaccharide unconjugated vaccine and a protein conjugated p
Externí odkaz:
https://doaj.org/article/17579a56ee1d469c858501c0b85717c7
Autor:
Ying Homan, Daniel Rosenbloom, Sally Wong, James Lucchese, April Li, Sheri Dubey, Justina Thomas, Gino Salituro, Roy Helmy, Thorsten Verch
Publikováno v:
Bioanalysis. 14:1177-1190
Aim: Critical virus reagents in regulated bioanalytical assays require stability monitoring. Although stability at ultra-low frozen temperatures is generally assumed, published data are limited and real-time studies are time consuming. Materials & me
Autor:
Tyler Chozinski, B. Scott Ferguson, William Fisher, Shencheng Ge, Qiang Gong, Hui Kang, John McDermott, Alexander Scott, Wentao Shi, Jeremiah J. Trausch, Thorsten Verch, Matthew Vukovich, Jinpeng Wang, J. Emma Wu, Qin Yang
Publikováno v:
Analytical Chemistry. 94:6146-6155
Global deployment of vaccines poses significant challenges in the distribution and use of the accompanying immunoassays, one of the standard methods for quality control of vaccines, particularly when establishing assays in countries worldwide to supp
Autor:
Thorsten Verch, Cristiana Campa, Cyrille C. Chéry, Ruth Frenkel, Timothy Graul, Nomalie Jaya, Bassam Nakhle, Jeremy Springall, Jason Starkey, Jette Wypych, Todd Ranheim
Publikováno v:
The AAPS journal. 24(1)
Analytical methods are utilized throughout the biopharmaceutical and vaccines industries to conduct research and development, and to help control manufacturing inputs and outputs. These analytical methods should continuously provide quality data to s
Autor:
Thorsten Verch, Mary Shank-Retzlaff, Richard R. Rustandi, Malorie Fink, Matthew C. Troutman, Carl Hofmann, Erin Marie Cannon, John W. Loughney, Cindy Pauley, Nisarg Patel
Publikováno v:
The AAPS journal. 22(6)
Monoclonal antibodies (mAbs) are widely used as critical reagents in analytical assays. While regulatory guidelines exist for stability monitoring of biopharmaceutical antibodies, they do not apply directly to the stability of mAbs used as assay reag
Publikováno v:
Bioanalysis. 10:163-180
Compared with biologics, vaccine potency assays represent a special challenge due to their unique compositions, multivalency, long life cycles and global distribution. Historically, vaccines were released using in vivo potency assays requiring immuni
Publikováno v:
Vaccine. 35:5495-5502
Vaccine in vitro potency assays are vital regulatory tests that are used to confirm the presence and concentration of an antigen of interest in a form that directly or indirectly relates to protective activity in patients. Current assays come in many
Publikováno v:
Analytical Chemistry. 89:3554-3561
Measuring vaccine potency is critical for vaccine release and is often accomplished using antibody-based ELISAs. Antibodies can be associated with significant drawbacks that are often overlooked including lot-to-lot variability, problems with cell-li
Autor:
Christopher Roselle, Dana Whitehouse, Mary Shank-Retzlaff, Sha-Ke Wang, Thorsten Verch, Silikhone Bouaraphan, Liming Guan, Thy Follmer, Fran Ansbro
Publikováno v:
Journal of Immunological Methods. 442:20-28
Dilutions are a common source of analytical error, both in terms of accuracy and precision, and a common source of analyst mistakes. When serial dilutions are used, errors compound, even when employing laboratory automation. Direct point dilutions in
Publikováno v:
Bioanalysis. 8:1451-1464
Background: Dilution bias is a major cause of immunoassay variability due to the lack of an internal standard to determine the true versus the expected dilution value. Methodology: We used an internal control to measure dilution bias in an ELISA. Acr