Zobrazeno 1 - 10
of 11
pro vyhledávání: '"Thorben, Schramm"'
Autor:
Thorben Schramm, Paul Lubrano, Vanessa Pahl, Amelie Stadelmann, Andreas Verhülsdonk, Hannes Link
Publikováno v:
Molecular Systems Biology, Vol 19, Iss 10, Pp n/a-n/a (2023)
Abstract Temperature‐sensitive (TS) mutants are a unique tool to perturb and engineer cellular systems. Here, we constructed a CRISPR library with 15,120 Escherichia coli mutants, each with a single amino acid change in one of 346 essential protein
Externí odkaz:
https://doaj.org/article/bdb550e782454880bb045c1aa6d4bd75
Autor:
Simon Boecker, Giulia Slaviero, Thorben Schramm, Witold Szymanski, Ralf Steuer, Hannes Link, Steffen Klamt
Publikováno v:
Molecular Systems Biology, Vol 17, Iss 12, Pp n/a-n/a (2021)
Abstract One long‐standing question in microbiology is how microbes buffer perturbations in energy metabolism. In this study, we systematically analyzed the impact of different levels of ATP demand in Escherichia coli under various conditions (aero
Externí odkaz:
https://doaj.org/article/c33789dc8d094bf5a81325973fec229e
Publikováno v:
J Bacteriol
In the Gram-positive model bacterium Bacillus subtilis, the presence of the amino acid glutamate triggers potassium uptake due to the glutamate-mediated activation of the potassium channel KtrCD. As a result, the intracellular accumulation of glutama
Flow-injection mass spectrometry (FI-MS) enables metabolomics studies with a very high sample-throughput. However, FI-MS is prone to in-source modifications of analytes because samples are directly injected into the electrospray ionization source of
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_________::077a1cad07a0148917af929603d7a846
https://doi.org/10.1101/2022.09.28.509873
https://doi.org/10.1101/2022.09.28.509873
Autor:
Maria G. Winter, Thorben Schramm, Sylwia Kierszniowska, Andrea Ebert-Jung, Sebastian E. Winter, Gottfried Unden, Hannes Link, Christopher Schubert, Kerstin Nagel-Wolfrum
Publikováno v:
Environmental Microbiology. 23:2564-2577
C4-dicarboxylates, such as fumarate, L-malate and L-aspartate represent substrates for anaerobic growth of Escherichia coli by fumarate respiration. Here, we determined whether C4-dicarboxylate metabolism as well as fumarate respiration contribute to
Autor:
Martin Lempp, Hannes Link, Timo Glatter, Silvia González Sierra, Thorben Schramm, Dominik Beuter
Publikováno v:
Metabolic Engineering
Controlling metabolism of engineered microbes is important to modulate cell growth and production during a bioprocess. For example, external parameters such as light, chemical inducers, or temperature can act on metabolism of production strains by ch
Autor:
Thorben Schramm, Hannes Link
Publikováno v:
BIOspektrum. 27:34-36
Cellular metabolism is very complex and extensively regulated. For many organisms we know almost the complete set of biochemical reactions in their metabolic network. However, it is not well understood how these reactions are regulated and how they i
Autor:
Christopher, Schubert, Maria, Winter, Andrea, Ebert-Jung, Sylwia, Kierszniowska, Kerstin, Nagel-Wolfrum, Thorben, Schramm, Hannes, Link, Sebastian, Winter, Gottfried, Unden
Publikováno v:
Environ Microbiol
Fumarate was previously known to serve as an anaerobic electron acceptor by E. coli when colonizing the mammalian intestine, but the source of that fumarate was elusive. In this issue, Unden and coworkers demonstrate that l-aspartic acid is the sourc
Publikováno v:
Analytical Chemistry. 89:1624-1631
Cellular metabolite concentrations hold information on the function and regulation of metabolic networks. However, current methods to measure metabolites are either low-throughput or not quantitative. Here we optimized conditions for liquid chromatog
Publikováno v:
Biotechnology Journal
The targeted increase of cellular adenosine triphosphate (ATP) turnover (enforced ATP wasting) has recently been recognized as a promising tool for metabolic engineering when product synthesis is coupled with net ATP formation. The goal of the presen