Zobrazeno 1 - 10
of 20
pro vyhledávání: '"Thor Las Holtet"'
Autor:
Mikkel Holmen Andersen, Hans Christian Thøgersen, Mette Munch, Thor Las Holtet, Hans Henrik Gad, Povilas Byla, Helle Jacobsen, Rune Hartmann
Publikováno v:
Journal of Biological Chemistry. 285:12096-12100
Inhibition of tumor necrosis factor α (TNFα) is a favorable way of treating several important diseases such as rheumatoid arthritis, Crohn disease, and psoriasis. Therefore, an extensive range of TNFα inhibitory proteins, most of them based upon a
Autor:
Thor Las Holtet, Troels Wind, Lisa Mathiasen, Peter A. Andreasen, Signe Kjelgaard, Anni Christensen, Grant E. Blouse, Helle H. Petersen, Martin Ejnar Hansen
Publikováno v:
Journal of Biological Chemistry. 280:38424-38437
To find new principles for inhibiting serine proteases, we screened phage-displayed random peptide repertoires with urokinase-type plasminogen activator (uPA) as the target. The most frequent of the isolated phage clones contained the disulfide bridg
Autor:
Charlotte H. Møller, Hans Christian Thøgersen, Rikke Høegh Lorentsen, Thor Las Holtet, Michael Etzerodt
Publikováno v:
Org. Biomol. Chem.. 1:1657-1663
A library of blood coagulation factor Xa (FXa)–trypsin hybrid proteases was generated and displayed on phage for selection of derivatives with the domain “architecture” of trypsin and the specificity of FXa. Selection based on binding to soybea
Autor:
Bettina Bryde Nielsen, Jonas Heilskov Graversen, Hans Christian Thøgersen, Michael Etzerodt, Thor Las Holtet, Jette S. Kastrup, Ingrid Kjøller Larsen, Hanne N. Rasmussen
Publikováno v:
Acta Crystallographica Section D Biological Crystallography. 54:757-766
Tetranectin (TN) is a C-type lectin involved in fibrinolysis, being the only endogenous ligand known to bind specifically to the kringle 4 domain of plasminogen. TN was originally isolated from plasma, but shows a wide tissue distribution. Furthermor
Autor:
Michael Etzerodt, Lars Ellgaard, Peter Reinholt Nielsen, Jørgen Gliemann, Thor Las Holtet, Hans Christian Thøgersen
Publikováno v:
European Journal of Biochemistry. 244:544-551
The alpha2macroglobulin-receptor-associated protein (RAP) binds to the alpha2macroglobulin receptor/low-density lipoprotein receptor-related protein (alpha2MR/LRP), a multi-functional cell surface receptor known to bind and internalize several macrom
Autor:
Kåre Lehmann Nielsen, Lars Sottrup-Jensen, Hans Christian Thøgersen, Thor Las Holtet, Michael Etzerodt, Søren K. Moestrup, Jørgen Gliemann
Publikováno v:
FEBS Letters. 344:242-246
A recombinant version of the receptor binding domain (RBDv) of human alpha 2-macroglobulin (alpha 2M) has been expressed in E. coli and refolded using a novel iterative procedure. RBDv (Val1299-Ala1451) is extended by 15 residues at the N-terminal si
Autor:
Hans Christian Thøgersen, Peter A. Andreasen, Michael Etzerodt, Anders Nykjaer, Lars Sottrup-Jensen, Thor Las Holtet, H. H. Rasmussen, Jørgen Gliemann, S K Moestrup
Publikováno v:
Moestrup, S K, Holtet, T L, Etzerodt, M, Thøgersen, H C, Nykjær, A, Andreasen, P, Rasmussen, H H, Sottrup-Jensen, L & Gliemann, J 1993, ' Alpha 2-macroglobulin-proteinase complexes, plasminogen activator inhibitor type-1-plasminogen activator complexes, and receptor-associated protein bind to a region of the alpha(2)-Macroglobulin receptor containing a cluster of eight complement-type repeats ', Journal of Biological Chemistry, vol. 268, no. 18, pp. 13691-13696 .
A region containing sites for ligand binding was localized in the 4525-amino acid residue alpha 2-macroglobulin receptor/low density lipoprotein receptor-related protein (alpha 2MR/LRP) by ligand- and immunoblotting of proteinase and CNBr digests of
Autor:
Thor Las Holtet, Niels Jørgen Olesen, P. E. Vestergård Jørgensen, Hans Christian Thøgersen, Michael Etzerodt, Niels Lorenzen
Publikováno v:
Lorenzen, N, Olesen, N J, Jørgensen, P E, Etzerodt, M, Holtet, T L & Thøgersen, H C 1993, ' Molecular cloning and expression in Escherichia coli of the glycoprotein gene of VHS virus, and immunization of rainbow trout with the recombinant protein ', Journal of General Virology, vol. 74, no. 4, pp. 623-630 .
The gene encoding the envelope glycoprotein of a recent Danish isolate of a salmonid rhabdovirus, viral haemorrhagic septicaemia virus (VHSV) has been cloned and sequenced at the cDNA level. When compared with the deduced sequence of a French isolate
Autor:
Bjarne Kuno Møller, Michael Etzerodt, Poul Henning Jensen, S K Moestrup, Peter A. Andreasen, Anders Nykjaer, Thor Las Holtet, Mette Munch, Hans Christian Thøgersen, Claus Munck Petersen
Publikováno v:
Journal of Biological Chemistry. 267:14543-14546
Complexes between 125I-labeled urokinase-type plasminogen activator (uPA) and plasminogen activator inhibitor type-1 (PAI-1) bound to purified alpha 2-macroglobulin (alpha 2M) receptor (alpha 2MR)/low density lipoprotein receptor-related protein (LRP
Autor:
Michael Etzerodt, Thor Las Holtet, Rikke Høegh Lorentsen, Charlotte Harkjaer Fynbo, Hans Christian Thøgersen
Publikováno v:
Protein expression and purification. 39(2)
Blood coagulation factor Xa (FXa) and Thrombin are well-known serine proteases often used for processing of recombinant fusion proteins, but because they are purified from bovine blood or other animal sources, there is a risk of pathogenic contaminan