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Directed Assembly of Homopentameric Cholera Toxin B‑Subunit Proteins into Higher-Order Structures Using Coiled-Coil Appendages

Autor: Ross, JF, Wildsmith, GC, Johnson, M, Hurdiss, DL, Hollingsworth, K, Thompson, RF, Mosayebi, M, Trinh, CH, Paci, E, Pearson, AR, Webb, ME, Turnbull, WB

Publikováno v: Ross, J F, Wildsmith, G C, Johnson, M, Hurdiss, D L, Hollingsworth, K, Thompson, R F, Mosayebi, M, Trinh, C H, Paci, E, Pearson, A R, Webb, M E & Turnbull, W B 2019, ' Directed Assembly of Homopentameric Cholera Toxin B-Subunit Proteins into Higher-Order Structures Using Coiled-Coil Appendages ', Journal of the American Chemical Society, vol. 141, no. 13, pp. 5211-5219 . https://doi.org/10.1021/jacs.8b11480
Journal of the American Chemical Society

The self-assembly of proteins into higher order structures is ubiquitous in living systems. It is also an essential process for the bottom-up creation of novel molecular architectures and devices for synthetic biology. However, the complexity of prot

Externí odkaz: https://explore.openaire.eu/search/publication?articleId=pmid_dedup__::621f253f4b4ffeb75759e8b66a5871ce
https://eprints.whiterose.ac.uk/143515/14/jacs.8b11480.pdf

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The structures of a naturally empty cowpea mosaic virus particle and its genome-containing counterpart by cryo-electron microscopy

Autor: Hesketh, EL, Meschcheriakova, Y, Thompson, RF, Lomonossoff, GP, Ranson, NA

Publikováno v: Scientific Reports, Vol 7, Iss 1, Pp 1-10 (2017)

Cowpea mosaic virus (CPMV) is a picorna-like plant virus. As well as an intrinsic interest in CPMV as a plant pathogen, CPMV is of major interest in biotechnology applications such as nanotechnology. Here, we report high resolution cryo electron micr

Externí odkaz: https://explore.openaire.eu/search/publication?articleId=dedup_wf_001::fad2a155b5b7443cf77c102890e798ee
https://eprints.whiterose.ac.uk/112969/14/art%3A10.1038%2Fs41598-017-00533-w.pdf

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neoepiscopeimproves neoepitope prediction with multi-variant phasing

Autor: Thompson Rf, Struck A, Ellrott K, Wood Ma, Nellore A, Nguyen A

The vast majority of tools for neoepitope prediction from DNA sequencing of complementary tumor and normal patient samples do not consider germline context or the potential for co-occurrence of two or more somatic variants on the same mRNA transcript

Externí odkaz: https://explore.openaire.eu/search/publication?articleId=doi_dedup___::36e3aad83365977920b58a7d28c9a4c5
https://doi.org/10.1101/418129

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Approaches to altering particle distributions in cryo-electron microscopy sample preparation

Autor: Drulyte, I, Johnson, RM, Hesketh, EL, Hurdiss, DL, Scarff, CA, Porav, SA, Ranson, NA, Muench, SP, Thompson, RF

Publikováno v: Acta Crystallographica. Section D, Structural Biology

This paper describes different approaches that cryo-EM users can take to improve the quality of their sample distribution and ice for high-resolution single-particle cryo-EM.
Cryo-electron microscopy (cryo-EM) can now be used to determine high-r

Externí odkaz: https://explore.openaire.eu/search/publication?articleId=pmid_dedup__::2322095dcb307789d4c29cf8d2af3002
https://eprints.whiterose.ac.uk/130715/15/ic5104.pdf

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Mechanisms of assembly and genome packaging in an RNA virus revealed by high-resolution cryo-EM

Autor: Hesketh, EL, Meshcheriakova, Y, Dent, KC, Saxena, P, Thompson, RF, Cockburn, JJ, Lomonossoff, GP, Ranson, NA

Publikováno v: Nature Communications

Cowpea mosaic virus is a plant-infecting member of the Picornavirales and is of major interest in the development of biotechnology applications. Despite the availability of >100 crystal structures of Picornavirales capsids, relatively little is known

Externí odkaz: https://explore.openaire.eu/search/publication?articleId=pmid_dedup__::746c61eacc930f401c04cd9bf9748c3a
http://europepmc.org/articles/PMC4682053

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