Zobrazeno 1 - 10
of 52
pro vyhledávání: '"Thomas Y. Shih"'
Publikováno v:
Cancer Letters. 75:19-26
The 12R/59T/116Y mutations have been shown to confer a dominant negative activity on H- ras oncogene (H- ras 116Y). To determine whether this event is unique for H- ras , we introduced the same mutations into K- ras oncogene. This mutant, K- ras 116Y
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_dedup___::ae9f1e04f8529e45b16b3537fab4d26b
https://doi.org/10.1016/0304-3835(93)90202-k
https://doi.org/10.1016/0304-3835(93)90202-k
Publikováno v:
Experimental Cell Research. 208:415-421
NIH3T3 cells carrying a dominant negative H-ras mutant 116Y acquired resistance to transformation by some PTK oncogenes, i.e., v-fes, v-abl, and v-fms, but were sensitive to viral ras and serine threonine kinase oncogenes, v-raf and v-mos. One clone,
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_dedup___::69b905064fd9e4f783179435af58b0f4
https://doi.org/10.1006/excr.1993.1263
https://doi.org/10.1006/excr.1993.1263
Publikováno v:
Molecular Carcinogenesis. 5:118-128
Transformation of cloned rat embryo fibroblast (CREF) cells with the wild-type 5 adenovirus (wtAd5) transforming genes E1A and E1B (which extend from 0 to 11.2 map units) results in morphologically transformed cells that exhibit an increased saturati
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_dedup___::c60d422dad9034d4d2aafd6e85aa703c
https://doi.org/10.1002/mc.2940050207
https://doi.org/10.1002/mc.2940050207
Publikováno v:
International journal of cancer. Supplement = Journal international du cancer. Supplement. 6
We have introduced point mutations in v-rasH to study their effects on biochemical and biological properties of the ras-encoded protein p21. Several of these mutant proteins do not bind GTP and thus lack GTPase activity, while others were shown to ha
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_dedup___::aafa8133ba10ffd5f89e5b5bbaa68ac5
https://pubmed.ncbi.nlm.nih.gov/2066184
https://pubmed.ncbi.nlm.nih.gov/2066184
Publikováno v:
Molecular and Cellular Biology. 5:1449-1455
The v-rasH oncogene of Harvey murine sarcoma virus encodes a 21,000-dalton p21 protein which has been expressed at a high level as a fusion protein in Escherichia coli. We have purified the p21 to over 90% in purity without the use of any detergent o
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_dedup___::33402c9980e4a20e83ddf4eaa0a14742
https://doi.org/10.1128/mcb.5.6.1449
https://doi.org/10.1128/mcb.5.6.1449
Publikováno v:
Journal of Virology. 42:253-261
The p21 transforming protein coded for by the v-ras gene of Harvey murine sarcoma virus (Ha-MuSV) migrates as a doublet band between 21,000 and 23,000 daltons during sodium dodecyl sulfate-polyacrylamide gel electrophoresis. The lower band of the dou
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_dedup___::ee5e5d2e88498b89a374b8a80eea6dab
https://doi.org/10.1128/jvi.42.1.253-261.1982
https://doi.org/10.1128/jvi.42.1.253-261.1982
Publikováno v:
Experimental Cell Research. 142:181-189
A kidney cell line (MDCK) retains an adenylate cyclase system sensitive to glucagon, vasopressin, isoproterenol and prostaglandin E1. The stimulatory effect of glucagon on cAMP production was selectively lost in a cloned line derived from MDCK cells
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_dedup___::a66756ec67a7eb3f20cf2fffd8cec90e
https://doi.org/10.1016/0014-4827(82)90421-9
https://doi.org/10.1016/0014-4827(82)90421-9
Publikováno v:
Molecular and Cellular Biology. 7:3092-3097
Point mutations of p21 proteins were constructed by oligonucleotide-directed mutagenesis of the v-rasH oncogene, which substituted amino acid residues within the nucleotide-binding consensus sequence, GXG GXGK. When the glycine residue at position 10
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_________::275429ac86e6185f859ebb7c59309a4f
https://doi.org/10.1128/mcb.7.9.3092
https://doi.org/10.1128/mcb.7.9.3092
Autor:
E H Chang, Ronald W. Ellis, J M Maryak, Edward M. Scolnick, Howard A. Young, Douglas R. Lowy, Thomas Y. Shih, Deborah DeFeo
Publikováno v:
Journal of Virology. 36:408-420
Detailed restriction endonuclease maps were developed for Harvey murine sarcoma virus (Ha-MuSV) DNA (clone H-1), molecularly closed at its unique EcoRI site in pBR322, for three nonoverlapping subgenomic HindIII clones which together span the entire
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_dedup___::316d1948dd625b0ea5d10528a3fde263
https://doi.org/10.1128/jvi.36.2.408-420.1980
https://doi.org/10.1128/jvi.36.2.408-420.1980
Publikováno v:
Proceedings of the National Academy of Sciences. 76:5355-5359
We have recently identified a 21,000-dalton protein, p21, coded for by Kirsten or Harvey murine sarcoma virus. On the basis of the results obtained with the p21 of a mutant of Kirsten sarcoma virus, temperature sensitive for the maintenance of transf
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_dedup___::fff29aeb4aeaa2103b4b91216fb20a89
https://doi.org/10.1073/pnas.76.10.5355
https://doi.org/10.1073/pnas.76.10.5355