Zobrazeno 1 - 9
of 9
pro vyhledávání: '"Thomas W. van Ravesteyn"'
Publikováno v:
PLoS Genetics, Vol 16, Iss 10, p e1009041 (2020)
Through transfection of short single-stranded oligodeoxyribonucleotides (ssODNs) small genomic alterations can be introduced into mammalian cells with high precision. ssODNs integrate into the genome during DNA replication, but the resulting heterodu
Externí odkaz:
https://doaj.org/article/6eb274cca1854357a90cfb1c141016cf
Autor:
Wouter de Laat, Anton van Weert, Sanne Boersma, Peter H.L. Krijger, Lieke I P M Donders, Jo Vandesompele, Rene J T M Roymans, Stefan Meijer, Francesca Mattiroli, Tim A. Hoek, Pim W. Toonen, Edwin Dekker, Monique Nijhuis, Thomas W van Ravesteyn, Ive Logister, Fred J Dom, Maaike Broeders, Mark Pieterse, Lieven B J van der Velden, Martijn Bosch, Bram M.P. Verhagen, Marvin E. Tanenbaum, Marjon J.A.M. Verstegen, Jeroen H.B. van de Bovenkamp, Rob Ruijtenbeek
Publikováno v:
Nature Biotechnology, 39(10), 1182-1184. Nature Publishing Group
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_dedup___::abf13f7adf6a0ecc214d0a147ddeb3bf
https://pure.knaw.nl/portal/en/publications/4f9be3a2-c913-455b-aeeb-b0f8bf0d8146
https://pure.knaw.nl/portal/en/publications/4f9be3a2-c913-455b-aeeb-b0f8bf0d8146
Autor:
Peter H L Krijger, Tim A Hoek, Sanne Boersma, Lieke I P M Donders, Maaike M C Broeders, Mark Pieterse, Pim W Toonen, Ive Logister, Bram M P Verhagen, Marjon J A M Verstegen, Thomas W van Ravesteyn, Rene J T M Roymans, Francesca Mattiroli, Jo Vandesompele, Monique Nijhuis, Stefan Meijer, Anton van Weert, Edwin Dekker, Fred J Dom, Rob Ruijtenbeek, Lieven B J van der Velden, Jeroen H B van de Bovenkamp, Martijn Bosch, Wouter de Laat, Marvin E Tanenbaum
An in-house multiplex RT-qPCR , targeting SARS-CoV-2and PDV as internal control [1][2], developed on QuantStudio 7 Pro Real-Time PCR Systems using Life Technologies Taqman FastVirus 1-step mastermix with E-gene primers and probe as described by Corma
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_________::606620bc45663e98f4a165e3c5597363
https://doi.org/10.17504/protocols.io.bxmjpk4n
https://doi.org/10.17504/protocols.io.bxmjpk4n
Autor:
Peter H L Krijger, Tim A Hoek, Sanne Boersma, Lieke I P M Donders, Maaike M C Broeders, Mark Pieterse, Pim W Toonen, Ive Logister, Bram M P Verhagen, Marjon J A M Verstegen, Thomas W van Ravesteyn, Rene J T M Roymans, Francesca Mattiroli, Jo Vandesompele, Monique Nijhuis, Stefan Meijer, Anton van Weert, Edwin Dekker, Fred J Dom, Rob Ruijtenbeek, Lieven B J van der Velden, Jeroen H B van de Bovenkamp, Martijn Bosch, Wouter de Laat, Marvin E Tanenbaum
STRIP is a start-to-end streamlined and automated procedure for COVID-19 testing, centering on a single Tecan Fluent liquid-handling robot that can process over 14,000 samples per day. The sensitivity, specificity, and practical implementation of STR
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_________::1c203b8159b49cfd50739c96f7f0bc9f
https://doi.org/10.17504/protocols.io.bxiwpkfe
https://doi.org/10.17504/protocols.io.bxiwpkfe
Autor:
Peter H L Krijger, Tim A Hoek, Sanne Boersma, Lieke I P M Donders, Maaike M C Broeders, Mark Pieterse, Pim W Toonen, Ive Logister, Bram M P Verhagen, Marjon J A M Verstegen, Thomas W van Ravesteyn, Rene J T M Roymans, Francesca Mattiroli, Jo Vandesompele, Monique Nijhuis, Stefan Meijer, Anton van Weert, Edwin Dekker, Fred J Dom, Rob Ruijtenbeek, Lieven B J van der Velden, Jeroen H B van de Bovenkamp, Martijn Bosch, Wouter de Laat, Marvin E Tanenbaum
STRIP is a start-to-end streamlined and automated procedure for COVID-19 testing, centering on a single Tecan Fluent liquid-handling robot that can process over 14,000 samples per day. Here we describe the protocol to prepare the magnetic beads that
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_________::bc785f05901788edbf0d2fa39f1858e3
https://doi.org/10.17504/protocols.io.bxixpkfn
https://doi.org/10.17504/protocols.io.bxixpkfn
Autor:
Peter H L Krijger, Tim A Hoek, Sanne Boersma, Lieke I P M Donders, Maaike M C Broeders, Mark Pieterse, Pim W Toonen, Ive Logister, Bram M P Verhagen, Marjon J A M Verstegen, Thomas W van Ravesteyn, Rene J T M Roymans, Francesca Mattiroli, Jo Vandesompele, Monique Nijhuis, Stefan Meijer, Anton van Weert, Edwin Dekker, Fred J Dom, Rob Ruijtenbeek, Lieven B J van der Velden, Jeroen H B van de Bovenkamp, Martijn Bosch, Wouter de Laat, Marvin E Tanenbaum
One of the most critical innovations within STRIP is the collection of samples in small tubes (0.8 ml compared with 5–15 ml in standard diagnostics) with virus-inactivating and RNA-stabilizing lysis buffer (InActiv Blue) spiked with a control RNA (
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_________::987d5fcd0a17bbdc3447218f41b4e1f8
https://doi.org/10.17504/protocols.io.buvenw3e
https://doi.org/10.17504/protocols.io.buvenw3e
Autor:
Hein te Riele, Marleen Dekker, Wietske Pieters, Jarnick Lusseveld, Hellen Houlleberghs, Robert M.W. Hofstra, S. Verhoef, Thomas W. van Ravesteyn
Publikováno v:
Journal of Medical Genetics, 57(5), 308-315. BMJ PUBLISHING GROUP
Journal of Medical Genetics, 57(5), 308-315. BMJ Publishing Group
Journal of Medical Genetics, 57(5), 308-315. BMJ Publishing Group
BackgroundInactivating mutations in the MLH1 DNA mismatch repair (MMR) gene underlie 42% of Lynch syndrome (LS) cases. LS is a cancer predisposition causing early onset colorectal and endometrial cancer. Nonsense and frameshift alterations unambiguou
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_dedup___::e52bbff13eb681541a33838bb383a537
https://hdl.handle.net/1887/3185050
https://hdl.handle.net/1887/3185050
Autor:
Gerrit A. Meijer, Flora E. van Leeuwen, Johan Hoeksel, Willemina R. R. Geurts-Giele, Winand N.M. Dinjens, Peggy N. Atmodimedjo, Petur Snaebjornsson, Efraim H. Rosenberg, Berthe M.P. Aleman, Jelle ten Hoeve, Hein te Riele, Thomas W. van Ravesteyn, Monique E. van Leerdam, Lisanne S. Rigter
Publikováno v:
Gut, 67(3), 447-455. BMJ Publishing Group
Gut, 67, 447-455
Rigter, L S, Snaebjornsson, P, Rosenberg, E H, Atmodimedjo, P N, Aleman, B M, ten Hoeve, J, Geurts-Giele, W R, van Ravesteyn, T W, Hoeksel, J, Meijer, G A, te Riele, H, van Leeuwen, F E, Dinjens, W N & van Leerdam, M E 2018, ' Double somatic mutations in mismatch repair genes are frequent in colorectal cancer after Hodgkin's lymphoma treatment ', Gut, vol. 67, no. 3, pp. 447-455 . https://doi.org/10.1136/gutjnl-2016-312608
Gut, 67, 3, pp. 447-455
Gut, 67, 447-455
Rigter, L S, Snaebjornsson, P, Rosenberg, E H, Atmodimedjo, P N, Aleman, B M, ten Hoeve, J, Geurts-Giele, W R, van Ravesteyn, T W, Hoeksel, J, Meijer, G A, te Riele, H, van Leeuwen, F E, Dinjens, W N & van Leerdam, M E 2018, ' Double somatic mutations in mismatch repair genes are frequent in colorectal cancer after Hodgkin's lymphoma treatment ', Gut, vol. 67, no. 3, pp. 447-455 . https://doi.org/10.1136/gutjnl-2016-312608
Gut, 67, 3, pp. 447-455
ObjectiveHodgkin's lymphoma survivors who were treated with infradiaphragmatic radiotherapy or procarbazine-containing chemotherapy have a fivefold increased risk of developing colorectal cancer (CRC). This study aims to provide insight into the deve
Autor:
Thomas W. van Ravesteyn, Titia K. Sixma, Alexander Fish, Astrid Wolters, Rob J. Dekker, Marleen Dekker, Hein te Riele
Publikováno v:
van Ravesteyn, T W, Dekker, M, Fish, A, Sixma, T K, Wolters, A, Dekker, R J & te Riele, HPJ 2016, ' LNA modification of single-stranded DNA oligonucleotides allows subtle gene modification in mismatch-repair-proficient cells ', Proceedings of the National Academy of Sciences of the United States of America, vol. 113, no. 15, pp. 4122-4127 . https://doi.org/10.1073/pnas.1513315113
Proceedings of the National Academy of Sciences of the United States of America, 113(15), 4122-4127. National Academy of Sciences
Proceedings of the National Academy of Sciences of the United States of America, 113(15), 4122-4127. National Academy of Sciences
Synthetic single-stranded DNA oligonucleotides (ssODNs) can be used to generate subtle genetic modifications in eukaryotic and prokaryotic cells without the requirement for prior generation of DNA double-stranded breaks. However, DNA mismatch repair