Polyether Ionophore Antibiotics Target Drug-Resistant Clinical Isolates, Persister Cells, and Biofilms

Autor: Malene Wollesen, Kasper Mikkelsen, Marie Selch Tvilum, Martin Vestergaard, Mikala Wang, Rikke L. Meyer, Hanne Ingmer, Thomas B. Poulsen, Thomas Tørring

Publikováno v: Microbiology Spectrum, Vol 11, Iss 4 (2023)

ABSTRACT Polyether ionophores are complex natural products known to transport various cations across biological membranes. While several members of this family are used in agriculture (e.g., as anti-coccidiostats) and have potent antibacterial activi

Externí odkaz: https://doaj.org/article/bcbdb837da96405c82180f4578ebe063

Using LanM Enzymes to Modify Glucagon-Like Peptides 1 and 2 inE.coli

Autor: Camilla Kjeldgaard Larsen, Peter Lindquist, Mette Rosenkilde, Alice Ravn Madsen, Kim Haselmann, Tine Glendorf, Kjeld Olesen, Anne Louise Bank Kodal, Thomas Tørring

Selective modification of peptides is often exploited to improve pharmaceutically relevant properties of bioactive peptides like stability, circulation time, and potency. In Nature, natural products belonging to the class of ribosomally synthesized a

Externí odkaz: https://explore.openaire.eu/search/publication?articleId=doi_________::ff5875e643365b69c45874bfbefbef2f
https://doi.org/10.1101/2023.03.20.532734

MIBiG 3.0:: a community-driven effort to annotate experimentally validated biosynthetic gene clusters

Autor: Barbara R Terlouw, Kai Blin, Jorge C Navarro-Muñoz, Nicole E Avalon, Marc G Chevrette, Susan Egbert, Sanghoon Lee, David Meijer, Michael J J Recchia, Zachary L Reitz, Jeffrey A van Santen, Nelly Selem-Mojica, Thomas Tørring, Liana Zaroubi, Mohammad Alanjary, Gajender Aleti, César Aguilar, Suhad A A Al-Salihi, Hannah E Augustijn, J Abraham Avelar-Rivas, Luis A Avitia-Domínguez, Francisco Barona-Gómez, Jordan Bernaldo-Agüero, Vincent A Bielinski, Friederike Biermann, Thomas J Booth, Victor J Carrion Bravo, Raquel Castelo-Branco, Fernanda O Chagas, Pablo Cruz-Morales, Chao Du, Katherine R Duncan, Athina Gavriilidou, Damien Gayrard, Karina Gutiérrez-García, Kristina Haslinger, Eric J N Helfrich, Justin J J van der Hooft, Afif P Jati, Edward Kalkreuter, Nikolaos Kalyvas, Kyo Bin Kang, Satria Kautsar, Wonyong Kim, Aditya M Kunjapur, Yong-Xin Li, Geng-Min Lin, Catarina Loureiro, Joris J R Louwen, Nico L L Louwen, George Lund, Jonathan Parra, Benjamin Philmus, Bita Pourmohsenin, Lotte J U Pronk, Adriana Rego, Devasahayam Arokia Balaya Rex, Serina Robinson, L Rodrigo Rosas-Becerra, Eve T Roxborough, Michelle A Schorn, Darren J Scobie, Kumar Saurabh Singh, Nika Sokolova, Xiaoyu Tang, Daniel Udwary, Aruna Vigneshwari, Kristiina Vind, Sophie P J M Vromans, Valentin Waschulin, Sam E Williams, Jaclyn M Winter, Thomas E Witte, Huali Xie, Dong Yang, Jingwei Yu, Mitja Zdouc, Zheng Zhong, Jérôme Collemare, Roger G Linington, Tilmann Weber, Marnix H Medema

Publikováno v: Nucleic Acids Research, 51(D1), D603-D610. Oxford University Press
Terlouw, B R, Blin, K, Navarro-Muñoz, J C, Avalon, N E, Chevrette, M G, Egbert, S, Lee, S, Meijer, D, Recchia, M J J, Reitz, ZL, van Santen, JA, Selem-Mojica, N, Tørring, T, Zaroubi, L, Alanjary, M, Aleti, G, Aguilar, C, Al-Salihi, S A A, Augustijn, HE, Avelar-Rivas, JA, Avitia-Domínguez, LA, Barona-Gómez, F, Bernaldo-Agüero, J, Bielinski, V A, Biermann, F, Booth, TJ, Carrion Bravo, VJ, Castelo-Branco, R, Chagas, FO, Cruz-Morales, P, Du, C, Duncan, KR, Gavriilidou, A, Gayrard, D, Gutiérrez-García, K, Haslinger, K, Helfrich, E J N, van der Hooft, J J J, Jati, AP, Kalkreuter, E, Kalyvas, N, Kang, KB, Kautsar, S, Kim, W, Kunjapur, AM, Li, Y-X, Lin, G-M, Loureiro, C, Louwen, J J R, Louwen, N L L, Lund, G, Parra, J, Philmus, B, Pourmohsenin, B, Pronk, L J U, Rego, A, Rex, DAB, Robinson, S, Rosas-Becerra, LR, Roxborough, ET, Schorn, MA, Scobie, DJ, Singh, KS, Sokolova, N, Tang, X, Udwary, D, Vigneshwari, A, Vind, K, Vromans, S P J M, Waschulin, V, Williams, SE, Winter, JM, Witte, TE, Xie, H, Yang, D, Yu, J, Zdouc, M, Zhong, Z, Collemare, J, Linington, RG, Weber, T & Medema, MH 2023, ' MIBiG 3.0: a community-driven effort to annotate experimentally validated biosynthetic gene clusters ', Nucleic Acids Research, vol. 51, no. D1, gkac1049, pp. D603-D610 . https://doi.org/10.1093/nar/gkac1049
Terlouw, B R, Blin, K, Navarro-Muñoz, J C, Avalon, N E, Chevrette, M G, Egbert, S, Lee, S, Meijer, D, Recchia, M J J, Reitz, Z L, van Santen, J A, Selem-Mojica, N, Tørring, T, Zaroubi, L, Alanjary, M, Aleti, G, Aguilar, C, Al-Salihi, S A A, Augustijn, H E, Avelar-Rivas, J A, Avitia-Domínguez, L A, Barona-Gómez, F, Bernaldo-Agüero, J, Bielinski, V A, Biermann, F, Booth, T J, Carrion Bravo, V J, Castelo-Branco, R, Chagas, F O, Cruz-Morales, P, Du, C, Duncan, K R, Gavriilidou, A, Gayrard, D, Gutiérrez-García, K, Haslinger, K, Helfrich, E J N, van der Hooft, J J J, Jati, A P, Kalkreuter, E, Kalyvas, N, Kang, K B, Kautsar, S, Kim, W, Kunjapur, A M, Li, Y-X, Lin, G-M, Loureiro, C, Louwen, J J R, Louwen, N L L, Lund, G, Parra, J, Philmus, B, Pourmohsenin, B, Pronk, L J U, Rego, A, Rex, D A B, Robinson, S, Rosas-Becerra, L R, Roxborough, E T, Schorn, M A, Scobie, D J, Singh, K S, Sokolova, N, Tang, X, Udwary, D, Vigneshwari, A, Vind, K, Vromans, S P J M, Waschulin, V, Williams, S E, Winter, J M, Witte, T E, Xie, H, Yang, D, Yu, J, Zdouc, M, Zhong, Z, Collemare, J, Linington, R G, Weber, T & Medema, M H 2023, ' MIBiG 3.0: a community-driven effort to annotate experimentally validated biosynthetic gene clusters ', Nucleic Acids Research, vol. 51, no. D1, pp. D603–D610 . https://doi.org/10.1093/nar/gkac1049
Terlouw, B R, Blin, K, Navarro-Muñoz, J C, Avalon, N E, Chevrette, M G, Egbert, S, Lee, S, Meijer, D, Recchia, M J J, Reitz, Z L, van Santen, J A, Selem-Mojica, N, Tørring, T, Zaroubi, L, Alanjary, M, Aleti, G, Aguilar, C, Al-Salihi, S A A, Augustijn, H E, Avelar-Rivas, J A, Avitia-Domínguez, L A, Barona-Gómez, F, Bernaldo-Agüero, J, Bielinski, V A, Biermann, F, Booth, T J, Carrion Bravo, V J, Castelo-Branco, R, Chagas, F O, Cruz-Morales, P, Du, C, Duncan, K R, Gavriilidou, A, Gayrard, D, Gutiérrez-García, K, Haslinger, K, Helfrich, E J N, van der Hooft, J J J, Jati, A P, Kalkreuter, E, Kalyvas, N, Kang, K B, Kautsar, S, Kim, W, Kunjapur, A M, Li, Y-X, Lin, G-M, Loureiro, C, Louwen, J J R, Louwen, N L L, Lund, G, Parra, J, Philmus, B, Pourmohsenin, B, Pronk, L J U, Rego, A, Rex, D A B, Robinson, S, Rosas-Becerra, L R, Roxborough, E T, Schorn, M A, Scobie, D J, Singh, K S, Sokolova, N, Tang, X, Udwary, D, Vigneshwari, A, Vind, K, Vromans, S P J M, Waschulin, V, Williams, S E, Winter, J M, Witte, T E, Xie, H, Yang, D, Yu, J, Zdouc, M, Zhong, Z, Collemare, J, Linington, R G, Weber, T & Medema, M H 2023, ' MIBiG 3.0 : a community-driven effort to annotate experimentally validated biosynthetic gene clusters ', Nucleic Acids Research, vol. 51, no. D1, pp. D603–D610 . https://doi.org/10.1093/nar/gkac1049
Nucleic acids research, 51(D1), D603-D610
Nucleic acids research 51 (2023) D1

With an ever-increasing amount of (meta)genomic data being deposited in sequence databases, (meta)genome mining for natural product biosynthetic pathways occupies a critical role in the discovery of novel pharmaceutical drugs, crop protection agents

Externí odkaz: https://explore.openaire.eu/search/publication?articleId=doi_dedup___::5e557bd5c3d2ddd7d6574fc9d08027c2
https://pure.knaw.nl/portal/en/publications/4e99309a-6840-4150-801f-070cfa20d40c

Expanding the antibacterial selectivity of polyether ionophore antibiotics through diversity-focused semisynthesis

Autor: Thomas Tørring, Jaime Moyano-Villameriel, Malene Wollesen, Thomas B. Poulsen, Christine N. Pedersen, Frederikke D. Andersen, Han Liu, Kristian M. Jacobsen, Peter Nørby, Shaoquan Lin, Esben B. Svenningsen

Publikováno v: Nature Chemistry
Nature chemistry
Lin, S, Liu, H, Svenningsen, E B, Wollesen, M, Jacobsen, K M, Andersen, F D, Moyano-Villameriel, J, Pedersen, C N, Nørby, P, Tørring, T & Poulsen, T B 2021, ' Expanding the antibacterial selectivity of polyether ionophore antibiotics through diversity-focused semisynthesis ', Nature Chemistry, vol. 13, no. 1, pp. 47-55 . https://doi.org/10.1038/s41557-020-00601-1

Polyether ionophores are complex natural products capable of transporting cations across biological membranes. Many polyether ionophores possess potent antimicrobial activity and a few selected compounds have the ability to target aggressive cancer c

Externí odkaz: https://explore.openaire.eu/search/publication?articleId=doi_dedup___::95445becf1000d7092cfae4f7e688d01
https://doi.org/10.1038/s41557-020-00601-1

Introduction of an Aldehyde Handle on Nanobodies by Affinity-Guided Labeling

Autor: Kasper R. Andersen, Thomas Tørring, Veronica Liv Andersen, Mikkel B. Skovsgaard, Thorbjørn B. Nielsen, Nick S. Laursen, Johan Palmfeldt, Kurt V. Gothelf, Michael Rosholm Mortensen, Anders Märcher, Jørgen Kjems

Publikováno v: Mortensen, M R, Skovsgaard, M B, Märcher, A, Andersen, V L, Palmfeldt, J, Nielsen, T B, Tørring, T, Laursen, N S, Andersen, K R, Kjems, J & Gothelf, K V 2020, ' Introduction of an Aldehyde Handle on Nanobodies by Affinity-Guided Labeling ', Bioconjugate Chemistry, vol. 31, no. 5, pp. 1295-1300 . https://doi.org/10.1021/acs.bioconjchem.0c00151

Chemically modified antigen-binding proteins are widely applied for their targeting abilities in the fields of biotechnology, medicine, and diagnostics. However, the production of site-selectively modified proteins remains a challenge. Here, we have

Externí odkaz: https://explore.openaire.eu/search/publication?articleId=doi_dedup___::626942fa10869db6f873003ab26174bc
https://pure.au.dk/portal/da/publications/introduction-of-an-aldehyde-handle-on-nanobodies-by-affinityguided-labeling(9a75013f-632d-45ff-b3f3-cf6128afef82).html