Zobrazeno 1 - 10
of 49
pro vyhledávání: '"Thomas P Pirone"'
Praised by The New York Times as'an indispensable guide for the homeowner and the professional,'Tree Maintenance has been the definitive source on maintenance of North American landscape trees for over fifty years, an essential reference not only for
Publikováno v:
Journal of Virological Methods. 77:11-15
The coding sequence for a series of six histidines (his-tag) was inserted near the 5′ terminus of the helper component (HC) coding region of tobacco etch potyvirus (TEV). Full length genomic clones containing the his-tag coding sequence were infect
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_dedup___::671bfbc11060362637e65b8e42b70dc9
https://doi.org/10.1016/s0166-0934(98)00085-8
https://doi.org/10.1016/s0166-0934(98)00085-8
Autor:
Thomas P. Pirone, John G. Shaw
The chapters in this book represent detailed versions of papers presented at the Symposium on Viral Genes and Plant Pathogenesis held at Lexington, Kentucky on October 16 and 17, 1989. In selecting topics and authors, we attempted to have represented
Autor:
Thomas P. Pirone, El Desouky Ammar, Stéphane Blanc, S. Garcia-Lampasona, J. Baker, Valerian V. Dolja, César Llave
Publikováno v:
Journal of General Virology. 79:3119-3122
Mutations of K --> E in the highly conserved 'KITC' motif of the potyvirus helper component (HC) protein result in loss of HC function in aphid transmission, presumably because of inability to interact with virions, stylets or both. In this study we
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_dedup___::685f7073bebceed39813be58d8e925e1
https://doi.org/10.1099/0022-1317-79-12-3119
https://doi.org/10.1099/0022-1317-79-12-3119
Autor:
Renyuan Wang, Sandra García-Lampasona, Thomas P. Pirone, David W. Thornbury, Stéphane Blanc, Juan José López-Moya
Publikováno v:
Virology. 231(1):141-147
Specific binding between the coat protein (CP) and the helper component (HC) of the tobacco vein mottling potyvirus (TVMV) was characterized using a protein blotting–overlay protocol. In thisin vitroassay, HC interacted with either virions or CP mo
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_dedup___::2c68bf0dea64029f738b1338ccee7782
Autor:
Stéphane Blanc, Thomas P. Pirone
Publikováno v:
Annual Review of Phytopathology. 34:227-247
▪ Abstract A variety of noncirculatively transmitted viruses have evolved a vector transmission strategy that involves, in addition to virions, virus-encoded proteins that are not constituents of virions. These “helpers” and the genes encoding
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_dedup___::618ff1eb57938edb717fc308a49551fd
https://doi.org/10.1146/annurev.phyto.34.1.227
https://doi.org/10.1146/annurev.phyto.34.1.227
Publikováno v:
Archives of Virology. 141:1535-1552
The Burley tobacco (Nicotiana tabacum) cultivar TN 86 is "resistant" to most strains of tobacco vein mottling potyvirus (TVMV), the virus being restricted to epidermal cells of inoculated leaves. One strain, designated TVMV-S, overcomes this resistan
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_dedup___::1de92b60e4c16482af52d00f92461610
https://doi.org/10.1007/bf01718253
https://doi.org/10.1007/bf01718253
Publikováno v:
Journal of General Virology. 74:2731-2735
The N-terminal portion (P1-HC-Pro-P3) of the tobacco vein mottling virus (TVMV) polyprotein was expressed in insect cells and larvae by a recombinant baculovirus. The proteases necessary to process this TVMV polyprotein fragment were active in insect
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_dedup___::b3b490ae80255028d5991b3d1a65482d
https://doi.org/10.1099/0022-1317-74-12-2731
https://doi.org/10.1099/0022-1317-74-12-2731
Publikováno v:
Virology. 191:106-111
Helper component (HC-Pro) is a virus-encoded nonstructural protein required for aphid transmission of potyviruses. In the tobacco vein mottling virus (TVMV) polyprotein, HC-Pro represents a 457 residue polypeptide from amino acid position 257 to 713.
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_dedup___::7c570fbadcf43667d4a8c06404c9416c
https://doi.org/10.1016/0042-6822(92)90171-k
https://doi.org/10.1016/0042-6822(92)90171-k
Publikováno v:
Plant Molecular Biology. 19:517-522
The use of polymerase chain reaction (PCR)-based mutagenesis to create chimeric genes is presently not cost-effective because of the size and number of primers as well as the number of PCRs required. We have developed two strategies based on inverse
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_dedup___::f78ab26fd73c0e983264fb935cfd7dbd
https://doi.org/10.1007/bf00023403
https://doi.org/10.1007/bf00023403