Zobrazeno 1 - 10
of 13
pro vyhledávání: '"Thomas O. Linden"'
The goal of this research is to leverage computational molecular biophysics to guide process development, reduce experimental burden and focus purification activities on feasible targets. Here, we distill a complex separation problem (e.g. chromatogr
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_________::395f03e52fca5fb8ad497abdadbe8cfd
https://doi.org/10.22541/au.159050658.84247547
https://doi.org/10.22541/au.159050658.84247547
Autor:
Matthew G. Petroff, Sunitha Kandula, Peter Machielsen, Thomas O. Linden, Haiying Bao, Nihal Tugcu, Miranda van Beuningen – de Vaan, Jennifer M. Pollard, John P. Welsh, David J. Roush
Publikováno v:
Biotechnology and Bioengineering. 113:1273-1283
High throughput experimental strategies are central to the rapid optimization of biologics purification processes. In this work, we extend common high throughput technologies towards the characterization of a multi-column chromatography process for a
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_________::128408eb170ed5e986b974ad19f09406
https://doi.org/10.1002/bit.25901
https://doi.org/10.1002/bit.25901
Autor:
Eggert Brekkan, David J. Roush, Karol Lacki, Jennifer M. Pollard, Haiying Bao, Thomas O. Linden, Kenneth Barlow, John P. Welsh
Publikováno v:
Engineering in Life Sciences. 16:160-168
Scale-down, high-throughput screening techniques are well on their way to becoming a commodity in downstream bioprocess development, especially for the rapid development of chromatography process steps. This work used both resin slurry plate and mini
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_________::e678b254bfac0b520c7bc1bf2286b980
https://doi.org/10.1002/elsc.201400251
https://doi.org/10.1002/elsc.201400251
Autor:
John P. Welsh, Michael A. Rauscher, Thomas O. Linden, Sandra Meissner, Haiying Bao, Jennifer M. Pollard, InKwan Han
Publikováno v:
Engineering in Life Sciences. 16:133-142
Application of scale-down high-throughput screening has become integral for process development of antibody therapeutic products. In this work, methods are described for using high-throughput techniques to develop a multicolumn chromatography purific
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_________::04b41996061b31ad5c262220f2bd1b49
https://doi.org/10.1002/elsc.201400255
https://doi.org/10.1002/elsc.201400255
Autor:
David Pollard, Jennifer M. Pollard, Hong Li, Jessika Feliciano, Thomas O. Linden, Matthew G. Petroff
Publikováno v:
Engineering in Life Sciences. 16:114-123
Upstream process development of biologics is not only productivity-driven but also quality-driven. Typically, most quality attributes are not directly measurable in cell culture samples due to low product concentration and purity, thus requiring some
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_________::c908cbcc348289c0525bdf5ca9e36f7a
https://doi.org/10.1002/elsc.201400250
https://doi.org/10.1002/elsc.201400250
Autor:
Haiying Bao, Matthew G. Petroff, John P. Welsh, Jennifer M. Pollard, David J. Roush, Patricia Rowicki, Thomas O. Linden
Publikováno v:
Biotechnology Progress. 30:626-635
The emergence of monoclonal antibody (mAb) therapies has created a need for faster and more efficient bioprocess development strategies in order to meet timeline and material demands. In this work, a high-throughput process development (HTPD) strateg
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_________::52d27c9ec0eb6afbec6ecda9a73ad6f9
https://doi.org/10.1002/btpr.1905
https://doi.org/10.1002/btpr.1905
Autor:
Sujatha Gomathinayagam, Nathan Sharkey, Peter M. Gray, Adam Nylen, Min Du, Thomas I. Potgieter, Seemab S. Shaikh, Raymond Evers, Terrance A. Stadheim, Robert C. Davidson, Russell B. Lingham, Julie A. DeMartino, Gavin C. Barnard, Daniel Hopkins, Muralidhar R. Mallem, Natarajan Sethuraman, Juergen H. Nett, Victoria Copeland, Thomas O. Linden, Marc d’Anjou, Yan Li, Liming Liu, Teresa Mitchell, Stephen R. Hamilton, Bing Gong, Denise M. Visco, Gail Forrest, Huijuan Li, Stefan Wildt
Publikováno v:
Journal of Biotechnology. 157:198-206
Pichia pastoris is a methylotropic yeast that has gained great importance as an organism for protein expression in recent years. Here, we report the expression of recombinant human erythropoietin (rhEPO) in glycoengineered P. pastoris. We show that g
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_dedup___::be5a467004a7cc4428d021a502528910
https://doi.org/10.1016/j.jbiotec.2011.11.002
https://doi.org/10.1016/j.jbiotec.2011.11.002
Autor:
Matthew G, Petroff, Haiying, Bao, John P, Welsh, Miranda, van Beuningen-de Vaan, Jennifer M, Pollard, David J, Roush, Sunitha, Kandula, Peter, Machielsen, Nihal, Tugcu, Thomas O, Linden
Publikováno v:
Biotechnology and bioengineering. 113(6)
High throughput experimental strategies are central to the rapid optimization of biologics purification processes. In this work, we extend common high throughput technologies towards the characterization of a multi-column chromatography process for a
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=pmid________::74f65dbf44fdf56b90a4d09613feda9e
https://pubmed.ncbi.nlm.nih.gov/26639315
https://pubmed.ncbi.nlm.nih.gov/26639315
Autor:
Esther Knieps, Jörg Thömmes, Maria-Regina Kula, Anders Ljunglöf, Jürgen Hubbuch, Thomas O. Linden
Publikováno v:
Journal of Chromatography A. 1021:93-104
An experimental study on the interplay of sorbent structure and fluid phase conditions (pH) has been carried out examining adsorption and transport of bovine serum albumin (BSA) and a monoclonal antibody (IgG 2a) on SP Sepharose Fast Flow and SP Seph
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_________::d74a4e200462d56affd4dabcb964e381
https://doi.org/10.1016/j.chroma.2003.08.112
https://doi.org/10.1016/j.chroma.2003.08.112
Publikováno v:
Journal of Chromatography A. 1021:105-115
The impact of different transport mechanism on chromatographic performance was studied by confocal laser scanning microscopy (CLSM) for solutions containing bovine serum albumin (BSA) and monoclonal IgG 2a under different solid- and fluid-phase condi
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_________::4cf643043ab76be6c4bb36d6f59a19ce
https://doi.org/10.1016/j.chroma.2003.08.092
https://doi.org/10.1016/j.chroma.2003.08.092