Use of Debye–Hückel–Henry charge measurements in early antibody development elucidates effects of non-specific association

Autor: Joshua R Laber, Thomas M Laue, Dana I Filoti

Publikováno v: Antibody Therapeutics. 5:211-215

The diffusion interaction parameter (kD) has been demonstrated to be a high-throughput technique for characterizing interactions between proteins in solution. kD reflects both attractive and repulsive interactions, including long-ranged electrostatic

Externí odkaz: https://explore.openaire.eu/search/publication?articleId=doi_dedup___::575125721035aefc5eedbc2e8c7309fb
https://doi.org/10.1093/abt/tbac018

Stoichiometry and Change of the mRNA Closed-Loop Factors as Translating Ribosomes Transit from Initiation to Elongation.

Autor: Xin Wang, Wen Xi, Shaun Toomey, Yueh-Chin Chiang, Jiri Hasek, Thomas M Laue, Clyde L Denis

Publikováno v: PLoS ONE, Vol 11, Iss 3, p e0150616 (2016)

Protein synthesis is a highly efficient process and is under exacting control. Yet, the actual abundance of translation factors present in translating complexes and how these abundances change during the transit of a ribosome across an mRNA remains u

Externí odkaz: https://doaj.org/article/4df0c9c4bf9843f6a56b654a73ae8063

A multilaboratory comparison of calibration accuracy and the performance of external references in analytical ultracentrifugation.

Autor: Huaying Zhao, Rodolfo Ghirlando, Carlos Alfonso, Fumio Arisaka, Ilan Attali, David L Bain, Marina M Bakhtina, Donald F Becker, Gregory J Bedwell, Ahmet Bekdemir, Tabot M D Besong, Catherine Birck, Chad A Brautigam, William Brennerman, Olwyn Byron, Agnieszka Bzowska, Jonathan B Chaires, Catherine T Chaton, Helmut Cölfen, Keith D Connaghan, Kimberly A Crowley, Ute Curth, Tina Daviter, William L Dean, Ana I Díez, Christine Ebel, Debra M Eckert, Leslie E Eisele, Edward Eisenstein, Patrick England, Carlos Escalante, Jeffrey A Fagan, Robert Fairman, Ron M Finn, Wolfgang Fischle, José García de la Torre, Jayesh Gor, Henning Gustafsson, Damien Hall, Stephen E Harding, José G Hernández Cifre, Andrew B Herr, Elizabeth E Howell, Richard S Isaac, Shu-Chuan Jao, Davis Jose, Soon-Jong Kim, Bashkim Kokona, Jack A Kornblatt, Dalibor Kosek, Elena Krayukhina, Daniel Krzizike, Eric A Kusznir, Hyewon Kwon, Adam Larson, Thomas M Laue, Aline Le Roy, Andrew P Leech, Hauke Lilie, Karolin Luger, Juan R Luque-Ortega, Jia Ma, Carrie A May, Ernest L Maynard, Anna Modrak-Wojcik, Yee-Foong Mok, Norbert Mücke, Luitgard Nagel-Steger, Geeta J Narlikar, Masanori Noda, Amanda Nourse, Tomas Obsil, Chad K Park, Jin-Ku Park, Peter D Pawelek, Erby E Perdue, Stephen J Perkins, Matthew A Perugini, Craig L Peterson, Martin G Peverelli, Grzegorz Piszczek, Gali Prag, Peter E Prevelige, Bertrand D E Raynal, Lenka Rezabkova, Klaus Richter, Alison E Ringel, Rose Rosenberg, Arthur J Rowe, Arne C Rufer, David J Scott, Javier G Seravalli, Alexandra S Solovyova, Renjie Song, David Staunton, Caitlin Stoddard, Katherine Stott, Holger M Strauss, Werner W Streicher, John P Sumida, Sarah G Swygert, Roman H Szczepanowski, Ingrid Tessmer, Ronald T Toth, Ashutosh Tripathy, Susumu Uchiyama, Stephan F W Uebel, Satoru Unzai, Anna Vitlin Gruber, Peter H von Hippel, Christine Wandrey, Szu-Huan Wang, Steven E Weitzel, Beata Wielgus-Kutrowska, Cynthia Wolberger, Martin Wolff, Edward Wright, Yu-Sung Wu, Jacinta M Wubben, Peter Schuck

Publikováno v: PLoS ONE, Vol 10, Iss 5, p e0126420 (2015)

Analytical ultracentrifugation (AUC) is a first principles based method to determine absolute sedimentation coefficients and buoyant molar masses of macromolecules and their complexes, reporting on their size and shape in free solution. The purpose o

Externí odkaz: https://doaj.org/article/3bd91e8f42734f39965350067490e003

Weak IgG self- and hetero-association characterized by fluorescence analytical ultracentrifugation

Autor: Danlin Yang, Andrew E. Nixon, Christopher J. Roberts, Sanjaya Singh, Thomas M. Laue, Walter F. Stafford, Rachel Kroe-Barrett, John J. Correia, David B. Hayes

Publikováno v: Protein Science. 27:1334-1348

Weak protein–protein interactions may be important to binding cooperativity. A panel of seven fluorescently labeled tracer monoclonal IgG antibodies, differing in variable (V) and constant (C) region sequences, were sedimented in increasing concent

Externí odkaz: https://explore.openaire.eu/search/publication?articleId=doi_________::5927e7e85028e9a9d5f3f2acc30d5c31
https://doi.org/10.1002/pro.3422

IgG cooperativity – Is there allostery? Implications for antibody functions and therapeutic antibody development

Autor: Sanjaya Singh, Danlin Yang, Thomas M. Laue, Christopher J. Roberts, Rachel Kroe-Barrett

Publikováno v: mAbs

A central dogma in immunology is that an antibody's in vivo functionality is mediated by 2 independent events: antigen binding by the variable (V) region, followed by effector activation by the constant (C) region. However, this view has recently bee

Externí odkaz: https://explore.openaire.eu/search/publication?articleId=doi_dedup___::01af86b94c69356ae675fbd9aa719dc7
https://doi.org/10.1080/19420862.2017.1367074