Zobrazeno 1 - 10
of 25
pro vyhledávání: '"Thomas M Norman"'
Autor:
Joseph M Replogle, Jessica L Bonnar, Angela N Pogson, Christina R Liem, Nolan K Maier, Yufang Ding, Baylee J Russell, Xingren Wang, Kun Leng, Alina Guna, Thomas M Norman, Ryan A Pak, Daniel M Ramos, Michael E Ward, Luke A Gilbert, Martin Kampmann, Jonathan S Weissman, Marco Jost
Publikováno v:
eLife, Vol 11 (2022)
CRISPR interference (CRISPRi) enables programmable, reversible, and titratable repression of gene expression (knockdown) in mammalian cells. Initial CRISPRi-mediated genetic screens have showcased the potential to address basic questions in cell biol
Externí odkaz:
https://doaj.org/article/af03cd98b4f34c128b98cd963fa2ba56
Autor:
Angela N Pogson, Jessica L Bonnar, Joseph M Replogle, Christina R Liem, Nolan K Maier, Yufang Ding, Baylee J Russell, Xingren Wang, Kun Leng, Alina Guna, Thomas M Norman, Ryan A Pak, Daniel M Ramos, Michael E Ward, Luke A Gilbert, Martin Kampmann, Jonathan S Weissman, Marco Jost
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_________::9e7d2d97d229edac14829d4e3858e296
https://doi.org/10.7554/elife.81856.sa2
https://doi.org/10.7554/elife.81856.sa2
Autor:
Joseph M. Replogle, Jessica L. Bonnar, Angela N. Pogson, Christina R. Liem, Nolan K. Maier, Yufang Ding, Baylee J. Russell, Xingren Wang, Kun Leng, Alina Guna, Thomas M. Norman, Ryan A. Pak, Daniel M. Ramos, Michael E. Ward, Luke A. Gilbert, Martin Kampmann, Jonathan S. Weissman, Marco Jost
CRISPR interference (CRISPRi) enables programmable, reversible, and titratable repression of gene expression (knockdown) in mammalian cells. Initial CRISPRi-mediated genetic screens have showcased the potential to address basic questions in cell biol
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_dedup___::9fdf7ca55256386579b422055fd4e823
https://doi.org/10.1101/2022.07.13.499814
https://doi.org/10.1101/2022.07.13.499814
Autor:
Melanie H Smith, Vianne R Gao, Michail Schizas, Alejandro Kochen, Edward F DiCarlo, Susan M Goodman, Thomas M Norman, Laura T Donlin, Christina S Leslie, Alexander Y Rudensky
Inflammation of non-barrier immunologically quiescent tissues is associated with a massive influx of blood-borne innate and adaptive immune cells. Cues from the latter are likely to alter and expand the spectrum of states observed in cells that are c
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_________::0e8d91335f69aa8022c0bd2d6c1104df
https://doi.org/10.1101/2022.02.28.482131
https://doi.org/10.1101/2022.02.28.482131
Autor:
Christoph Bock, Paul Datlinger, Florence Chardon, Matthew A. Coelho, Matthew B. Dong, Keith A. Lawson, Tian Lu, Laetitia Maroc, Thomas M. Norman, Bicna Song, Geoff Stanley, Sidi Chen, Mathew Garnett, Wei Li, Jason Moffat, Lei S. Qi, Rebecca S. Shapiro, Jay Shendure, Jonathan S. Weissman, Xiaowei Zhuang
Publikováno v:
Nat Rev Methods Primers
Nature Reviews Methods Primers
Nature Reviews Methods Primers
CRISPR screens are a powerful source of biological discovery, enabling the unbiased interrogation of gene function in a wide range of applications and species. In pooled CRISPR screens, various genetically encoded perturbations are introduced into po
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_dedup___::9149102bd77a1ee869f9486b6a475000
https://europepmc.org/articles/PMC10200264/
https://europepmc.org/articles/PMC10200264/
Autor:
Joseph M. Replogle, Reuben A. Saunders, Angela N. Pogson, Jeffrey A. Hussmann, Alexander Lenail, Alina Guna, Lauren Mascibroda, Eric J. Wagner, Karen Adelman, Gila Lithwick-Yanai, Nika Iremadze, Florian Oberstrass, Doron Lipson, Jessica L. Bonnar, Marco Jost, Thomas M. Norman, Jonathan S. Weissman
Publikováno v:
Cell. 185:2559-2575.e28
A central goal of genetics is to define the relationships between genotypes and phenotypes. High-content phenotypic screens such as Perturb-seq (pooled CRISPR-based screens with single-cell RNA-sequencing readouts) enable massively parallel functiona
Autor:
Jonathan S. Weissman, Thomas M. Norman, Jeffrey A. Hussmann, Daniel Assis Santos, Marco Jost, Carol A. Gross, Max A. Horlbeck, Christina R. Liem, Sonia M. Scaria, John S. Hawkins, Reuben A. Saunders
Publikováno v:
Nature biotechnology, vol 38, iss 3
Nature biotechnology
Nature biotechnology
A lack of tools to precisely control gene expression has limited our ability to evaluate relationships between expression levels and phenotypes. Here, we describe an approach to titrate expression of human genes using CRISPR interference and series o
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_dedup___::b1a18424dceee00a556f6e21f73690f5
https://escholarship.org/uc/item/14s573qj
https://escholarship.org/uc/item/14s573qj
Autor:
Jonathan S. Weissman, John S. Hawkins, Christina R. Liem, Marco Jost, Sonia M. Scaria, Max A. Horlbeck, Reuben A. Saunders, Thomas M. Norman, Daniel Assis Santos, Jeffrey A. Hussmann, Carol A. Gross
Biological phenotypes arise from the degrees to which genes are expressed, but the lack of tools to precisely control gene expression limits our ability to evaluate the underlying expression-phenotype relationships. Here, we describe a readily implem
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_dedup___::90fd8a5913c7c7fe903b65b3206b68c2
Autor:
Alexander Meissner, Marco Jost, Helene Kretzmer, Jonathan S. Weissman, Zachary D. Smith, Stefanie Grosswendt, Dian Yang, Thomas M. Norman, Alex Khodaverdian, Michelle Chan, Nir Yosef, Britt Adamson, Jeffrey J. Quinn, Matthew G. Jones
Publikováno v:
Nature, vol 570, iss 7759
Nature
Nature
Ontogeny describes the emergence of complex multicellular organisms from single totipotent cells. This field is particularly challenging in mammals, owing to the indeterminate relationship between self-renewal and differentiation, variation in progen
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_dedup___::2c2c62afa82544b63aebd711211d8b2b
https://escholarship.org/uc/item/7b11n471
https://escholarship.org/uc/item/7b11n471
Autor:
Max A. Horlbeck, Thomas M. Norman, Joseph M. Replogle, Albert Xu, Jonathan S. Weissman, Alex Y. Ge, Luke A. Gilbert, Marco Jost
Synergistic interactions between gene functions drive cellular complexity. However, the combinatorial explosion of possible genetic interactions (GIs) has necessitated the use of scalar interaction readouts (e.g. growth) that conflate diverse outcome
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_dedup___::fb5148eed952edf4e0194003802bd39a