Control of intracellular pH and bicarbonate by CO2 diffusion into human sperm

Autor: Elena Grahn, Svenja V. Kaufmann, Malika Askarova, Momchil Ninov, Luisa M. Welp, Thomas K. Berger, Henning Urlaub, U.Benjamin Kaupp

Publikováno v: Nature Communications, Vol 14, Iss 1, Pp 1-17 (2023)

Abstract The reaction of CO2 with H2O to form bicarbonate (HCO3 −) and H+ controls sperm motility and fertilization via HCO3 −-stimulated cAMP synthesis. A complex network of signaling proteins participates in this reaction. Here, we identify key

Externí odkaz: https://doaj.org/article/5299a3c7a08b44bc9f3ec95e8cfcad72

Species-specific differences in nonlysosomal glucosylceramidase GBA2 function underlie locomotor dysfunction arising from loss-of-function mutations

Autor: Hussein Hamzeh, Katharina Gutbrod, Matthias Geyer, Wolfgang Bönigk, Elena Grahn, Diana N. Raju, Heike Endepols, Heinz G. Körschen, Frank Bradke, Dominik Dittmann, Thomas K. Berger, Johannes M. F. G. Aerts, Marina A. Woeste, Peter Dörmann, Jan N. Hansen, Carina E. Marx, Roger Sandhoff, Dagmar Wachten, Sina Stern, Sophie Schonauer

Publikováno v: The Journal of Biological Chemistry
The journal of biological chemistry 294(11), 3853-3871 (2019). doi:10.1074/jbc.RA118.006311
Journal of Biological Chemistry, 294(11), 3853-3871. AMER SOC BIOCHEMISTRY MOLECULAR BIOLOGY INC

The nonlysosomal glucosylceramidase β2 (GBA2) catalyzes the hydrolysis of glucosylceramide to glucose and ceramide. Mutations in the human GBA2 gene have been associated with hereditary spastic paraplegia (HSP), autosomal-recessive cerebellar ataxia

Externí odkaz: https://explore.openaire.eu/search/publication?articleId=doi_dedup___::3d15045280ed8ce4c2e5661f40ffa912
https://doi.org/10.1074/jbc.ra118.006311

A family of hyperpolarization-activated channels selective for protons

Autor: Lea Wobig, Reinhard Seifert, Heinz G. Körschen, Sylvia Fechner, Thomas K. Berger, U. Benjamin Kaupp, Jan F. Jikeli, Regina Feederle, Wolfgang Bönigk, Christian Trötschel, Thérèse Wolfenstetter

Publikováno v: Proceedings of the National Academy of Sciences of the United States of America

Significance We discovered a subfamily of voltage-gated ion channels, called HCN-like channels, consisting of two members, HCNL1 and HCNL2. In contrast to classic pacemaker HCN channels in the heart and brain, HCNL1 conducts protons rather than potas

Externí odkaz: https://explore.openaire.eu/search/publication?articleId=doi_dedup___::69d4f4383f458393d4a05ff6920d390a
https://hdl.handle.net/21.11116/0000-0006-7C87-721.11116/0000-0007-640F-9

Effective stimuli for constructing reliable neuron models.

Autor: Shaul Druckmann, Thomas K Berger, Felix Schürmann, Sean Hill, Henry Markram, Idan Segev

Publikováno v: PLoS Computational Biology, Vol 7, Iss 8, p e1002133 (2011)

The rich dynamical nature of neurons poses major conceptual and technical challenges for unraveling their nonlinear membrane properties. Traditionally, various current waveforms have been injected at the soma to probe neuron dynamics, but the rationa

Externí odkaz: https://doaj.org/article/2496e65b7a6c4728b48a3f7e0e0f5d0d

Post-translational cleavage of Hv1 in human sperm tunes pH- and voltage-dependent gating

Autor: Thomas K. Berger, Christoph Brenker, Dagmar Wachten, U. Benjamin Kaupp, Timo Strünker, Normann Goodwin, Eberhard Krause, David M. Fußhöller, Astrid Müller, Nasim Dokani Khesroshahi, Wolfgang Bönigk

Publikováno v: The Journal of Physiology. 595:1533-1546

Key points In human sperm, proton flux across the membrane is controlled by the voltage-gated proton channel Hv1. We show that sperm harbour both Hv1 and an N-terminally cleaved isoform termed Hv1Sper. The pH-control of Hv1Sper and Hv1 is distinctive

Externí odkaz: https://explore.openaire.eu/search/publication?articleId=doi_________::6da9b7ee07c741fe605d9c9205f9da30
https://doi.org/10.1113/jp273189