Zobrazeno 1 - 10
of 30
pro vyhledávání: '"Thomas J. Lie"'
Publikováno v:
Microbiology Spectrum, Vol 10, Iss 1 (2022)
ABSTRACT Methanocaldococcus sp. strain FS406-22, a hyperthermophilic methanogen, fixes nitrogen with a minimal set of known nif genes. Only four structural nif genes, nifH, nifD, nifK, and nifE, are present in a cluster, and a nifB homolog is present
Externí odkaz:
https://doaj.org/article/d676da832f7642a79a5f9ac18b5adfb9
Publikováno v:
mBio, Vol 4, Iss 2 (2013)
ABSTRACT Hydrogenotrophic methanogenic Archaea require reduced ferredoxin as an anaplerotic source of electrons for methanogenesis. H2 oxidation by the hydrogenase Eha provides these electrons, consistent with an H2 requirement for growth. Here we re
Externí odkaz:
https://doaj.org/article/8c1756233a9b4953acf48bd5038ec03e
Autor:
Taylan Tunçkanat, Aleksei Gendron, Zoie Sadler, Alex Neitz, Sarah Byquist, Thomas J. Lie, Kylie D. Allen
Publikováno v:
Biochemistry. 61:1077-1090
Many methanogenic archaea synthesize β-amino acids as osmolytes that allow survival in high salinity environments. Here, we investigated the radical
Publikováno v:
Journal of Materials Research and Technology, Vol 31, Iss , Pp 2762-2773 (2024)
Type 420 martensitic stainless-steel powder was clad onto a CF3M austenitic stainless steel substrate using laser direct-energy-deposition (L-DED). Through comprehensive characterization and numerical simulation, the evolution of microstructures, par
Externí odkaz:
https://doaj.org/article/9d3d93ae8dd64c12ae7e0f8732a506c9
Publikováno v:
International Journal of Hydrogen Energy. 46:3742-3752
The Rnf (Rhodobacter nitrogen fixation) complex encoded by rnfABCDGEH genes is a crucial enzyme for the functioning of nitrogenase. In this work, we studied the effect of Rnf complex expression level on hydrogen production in the hydrogen-producing b
Autor:
Nathan D. Price, Juan Zhang, Thomas J. Lie, Matthew A. Richards, John A. Leigh, Stephen W. Ragsdale
Publikováno v:
Journal of Bacteriology
Hydrogenotrophic methanogenesis occurs in multiple environments, ranging from the intestinal tracts of animals to anaerobic sediments and hot springs. Energy conservation in hydrogenotrophic methanogens was long a mystery; only within the last decade
Autor:
Nitin S. Baliga, Kyle C. Costa, Joseph Slagel, Robert L. Moritz, David J Reiss, Min Pan, John A. Leigh, Serdar Turkarslan, Thomas J. Lie, Murray Hackett, June A. Burn, Sung Ho Yoon
Publikováno v:
Genome Research. 23:1839-1851
Methanogens catalyze the critical methane-producing step (called methanogenesis) in the anaerobic decomposition of organic matter. Here, we present the first predictive model of global gene regulation of methanogenesis in a hydrogenotrophic methanoge
Autor:
Kyle C. Costa, Suresh Korpole, William B. Whitman, Thomas J. Lie, John A. Leigh, Boguslaw Lupa
Publikováno v:
Proceedings of the National Academy of Sciences. 109:15473-15478
Despite decades of study, electron flow and energy conservation in methanogenic Archaea are still not thoroughly understood. For methanogens without cytochromes, flavin-based electron bifurcation has been proposed as an essential energy-conserving me
Autor:
Andrew Haydock, John A. Leigh, Brian C. Moore, Erik L. Hendrickson, Thomas J. Lie, Ulf M. Niess
Publikováno v:
Molecular Microbiology. 75:755-762
The euryarchaeal transcriptional repressor NrpR regulates a variety of nitrogen assimilation genes by 2-oxoglutarate-reversible binding to conserved palindromic operators. The number and positioning of these operators varies among promoter regions of
Autor:
John A. Leigh, Thomas J. Lie
Publikováno v:
Applied and Environmental Microbiology. 73:6595-6600
Reporter gene-based genetic screens for regulatory mutations have enjoyed wide utility in the Bacteria but have had limited use in the Archaea, due in part to the extreme growth conditions typically required by these organisms. However, effective too