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pro vyhledávání: '"Thomas Hellerer"'
Autor:
Thomas Kellerer, Janko Janusch, Christian Freymüller, Adrian Rühm, Ronald Sroka, Thomas Hellerer
Publikováno v:
International Journal of Molecular Sciences; Volume 23; Issue 24; Pages: 15885
Having access to fluorescence lifetime, researchers can reveal in-depth details about the microenvironment as well as the physico-chemical state of the molecule under investigation. However, the high number of influencing factors might be an explanat
Autor:
Amelie Erben, Thomas Kellerer, Josefine Lissner, Constanze Eulenkamp, Thomas Hellerer, null Hauke-Clausen-Schaumann, Stefanie Sudhop, Michael Heymann
Bioinspired 3D microfluidic systems that combine vascularization with extracellular matrix architectures of organotypic geometry, composition and biophysical traits can help advance our understanding of microorgan physiology. Here, two-photon stereol
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_________::03b4cedf6fefb0b638dca4bf067ff97f
https://doi.org/10.1101/2022.03.16.484492
https://doi.org/10.1101/2022.03.16.484492
Autor:
Mojtaba Mohseni, Markus Hilleringmann, Christoph Polzer, Thomas Kellerer, Joachim O. Rädler, Thomas Hellerer, Stefan Ness
Publikováno v:
Biomedical optics express. 10(9)
We present a two-color two-photon stimulated emission depletion microscopy technique (2C2P-STED) that correlates a confocal image with a super-resolved image employing the inherent self-referencing mechanism of nonlinear excitation. The novel approac
Autor:
Thomas Hellerer, Stefan Ness, Joachim O. Rädler, Markus Hilleringmann, Christoph Polzer, Mojtaba Mohseni
Publikováno v:
Multiphoton Microscopy in the Biomedical Sciences XIX.
We present numerical simulations and experiments employing two-photon excited fluorescence in a sum-frequency mixing scheme which could be used for colocalization experiments in biophysics. By means of numerical calculations using the Debye approxima
Publikováno v:
Optics Letters. 44:5218
In this Letter, we present an all-fiber, easy-to-use, wavelength-tunable, ultrafast laser, based on soliton self-frequency shifting in an Er-doped polarization-maintaining very large mode area fiber. The system is tunable over 370 nm, starting at 162
Publikováno v:
Optics Express. 26:10230
In this manuscript, we present a detailed investigation of the impact of dispersion on the spectral resolution achievable by the application of spectral focusing in coherent Raman imaging. Our results reveal the detrimental effect of third order disp
Autor:
Thomas Hellerer
Publikováno v:
Optik & Photonik. 3:35-38
Femtosecond lasers had an unpredictable success in optical sciences ever since they were introduced. Today physicists are still pushing the limits of ultrafast lasers, e.g. the pulse duration down to a single optical cycle and the peak power up to te
Publikováno v:
Proceedings of the National Academy of Sciences. 104:14658-14663
Better understanding of the fundamental mechanisms behind metabolic diseases requires methods to monitor lipid stores on single-cell level in vivo . We have used Caenorhabditis elegans as a model organism to demonstrate the limitations of fluorescenc
Publikováno v:
ChemPhysChem. 3:630-633
KEYWORDS:CARS ¥ correlation spectroscopy ¥ diffusion ¥ microscopy ¥nonlinearopticsCorrelationtechniqueshavebeenwidelyappliedformonitoringsignalfluctuationsinadefinedobservationvolume.Thecauseofthefluctuationscaneitherbechangesinthenumberdensityof
Publikováno v:
Applied Physics Letters. 85:25-27
In this work, we show how broad-bandwidth femtosecond pulses can be used to achieve high spectral resolution in nonlinear spectroscopy and microscopy. Our approach is based on chirping the excitation pulses in order to focus their entire bandwidth in